3D Electro-Rotation of Single Cells

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Release : 2022-05-31
Genre : Technology & Engineering
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Book Rating : 661/5 ( reviews)

3D Electro-Rotation of Single Cells - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook 3D Electro-Rotation of Single Cells write by Liang Huang. This book was released on 2022-05-31. 3D Electro-Rotation of Single Cells available in PDF, EPUB and Kindle. Dielectrophoresis microfluidic chips have been widely used in various biological applications due to their advantages of convenient operation, high throughput, and low cost. However, most of the DEP microfluidic chips are based on 2D planar electrodes which have some limitations, such as electric field attenuation, small effective working regions, and weak DEP forces. In order to overcome the limitations of 2D planar electrodes, two kinds of thick-electrode DEP chips were designed to realize manipulation and multi-parameter measurement of single cells. Based on the multi-electrode structure of thick-electrode DEP, a single-cell 3D electro-rotation chip of "Armillary Sphere" was designed. The chip uses four thick electrodes and a bottom planar electrode to form an electric field chamber, which can control 3D rotation of single cells under different electric signal configurations. Electrical property measurement and 3D image reconstruction of single cells are achieved based on single-cell 3D rotation. This work overcomes the limitations of 2D planar electrodes and effectively solves the problem of unstable spatial position of single-cell samples, and provides a new platform for single-cell analysis. Based on multi-electrode structure of thick-electrode DEP, a microfluidic chip with optoelectronic integration was presented. A dual-fiber optical stretcher embedded in thick electrodes can trap and stretch a single cell while the thick electrodes are used for single-cell rotation. Stretching and rotation manipulation gives the chip the ability to simultaneously measure mechanical and electrical properties of single cells, providing a versatile platform for single-cell analysis, further extending the application of thick-electrode DEP in biological manipulation and analysis.

3D Electro-Rotation of Single Cells

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Author :
Release : 2019-12-26
Genre : Technology & Engineering
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Book Rating : 926/5 ( reviews)

3D Electro-Rotation of Single Cells - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook 3D Electro-Rotation of Single Cells write by Liang Huang. This book was released on 2019-12-26. 3D Electro-Rotation of Single Cells available in PDF, EPUB and Kindle. Dielectrophoresis microfluidic chips have been widely used in various biological applications due to their advantages of convenient operation, high throughput, and low cost. However, most of the DEP microfluidic chips are based on 2D planar electrodes which have some limitations, such as electric field attenuation, small effective working regions, and weak DEP forces. In order to overcome the limitations of 2D planar electrodes, two kinds of thick-electrode DEP chips were designed to realize manipulation and multi-parameter measurement of single cells. Based on the multi-electrode structure of thick-electrode DEP, a single-cell 3D electro-rotation chip of "Armillary Sphere" was designed. The chip uses four thick electrodes and a bottom planar electrode to form an electric field chamber, which can control 3D rotation of single cells under different electric signal configurations. Electrical property measurement and 3D image reconstruction of single cells are achieved based on single-cell 3D rotation. This work overcomes the limitations of 2D planar electrodes and effectively solves the problem of unstable spatial position of single-cell samples, and provides a new platform for single-cell analysis. Based on multi-electrode structure of thick-electrode DEP, a microfluidic chip with optoelectronic integration was presented. A dual-fiber optical stretcher embedded in thick electrodes can trap and stretch a single cell while the thick electrodes are used for single-cell rotation. Stretching and rotation manipulation gives the chip the ability to simultaneously measure mechanical and electrical properties of single cells, providing a versatile platform for single-cell analysis, further extending the application of thick-electrode DEP in biological manipulation and analysis.

Microfluidics in Cell Biology Part C: Microfluidics for Cellular and Subcellular Analysis

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Release : 2018-11-22
Genre : Science
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Book Rating : 855/5 ( reviews)

Microfluidics in Cell Biology Part C: Microfluidics for Cellular and Subcellular Analysis - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Microfluidics in Cell Biology Part C: Microfluidics for Cellular and Subcellular Analysis write by . This book was released on 2018-11-22. Microfluidics in Cell Biology Part C: Microfluidics for Cellular and Subcellular Analysis available in PDF, EPUB and Kindle. Microfluidics in Cell Biology Part C, Volume 148, a new release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various multi-cellular models, including microfluidics in cell monolayers/spheroids, microfluidics in organ on chips, and microfluidics in model organisms. Specific chapters discuss collective migration in microtubes, leukocyte adhesion dynamics on endothelial monolayers under flow, constrained spheroid for perfusion culture, cells in droplet arrays, heart on chips, kidney on chips, liver on chips, and more. Contains contributions from experts in the field from across the world Covers a wide array of topics on both mitosis and meiosis Includes relevant, analysis based topics

Robotics for Cell Manipulation and Characterization

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Release : 2023-04-20
Genre : Computers
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Book Rating : 143/5 ( reviews)

Robotics for Cell Manipulation and Characterization - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Robotics for Cell Manipulation and Characterization write by Changsheng Dai. This book was released on 2023-04-20. Robotics for Cell Manipulation and Characterization available in PDF, EPUB and Kindle. Robotics for Cell Manipulation and Characterization provides fundamental principles underpinning robotic cell manipulation and characterization, state-of-the-art technical advances in micro/nano robotics, new discoveries of cell biology enabled by robotic systems, and their applications in clinical diagnosis and treatment. This book covers several areas, including robotics, control, computer vision, biomedical engineering and life sciences using understandable figures and tables to enhance readers’ comprehension and pinpoint challenges and opportunities for biological and biomedical research. Focuses on, and comprehensively covers, robotics for cell manipulation and characterization Highlights recent advances in cell biology and disease treatment enabled by robotic cell manipulation and characterization Provides insightful outlooks on future challenges and opportunities

Single Cell Analysis in Biotechnology and Systems Biology

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Release : 2018-10-01
Genre : Science
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Book Rating : 936/5 ( reviews)

Single Cell Analysis in Biotechnology and Systems Biology - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Single Cell Analysis in Biotechnology and Systems Biology write by Fan-Gang Tseng. This book was released on 2018-10-01. Single Cell Analysis in Biotechnology and Systems Biology available in PDF, EPUB and Kindle. This book is a printed edition of the Special Issue "Single Cell Analysis in Biotechnology and Systems Biology" that was published in IJMS