Advanced Fluorescence Reporters in Chemistry and Biology III - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Advanced Fluorescence Reporters in Chemistry and Biology III write by Alexander P. Demchenko. This book was released on 2011-03-29. Advanced Fluorescence Reporters in Chemistry and Biology III available in PDF, EPUB and Kindle. The key element of any fluorescence sensing or imaging technology is the fluorescence reporter, which transforms the information on molecular interactions and dynamics into measurable signals of fluorescence emission. This book, written by a team of frontline researchers, demonstrates the broad field of applications of fluorescence reporters, starting from nanoscopic properties of materials, such as self-assembled thin films, polymers and ionic liquids, through biological macromolecules and further to living cell, tissue and body imaging. Basic information on obtaining and interpreting experimental data is presented and recent progress in these practically important areas is highlighted. The book is addressed to a broad interdisciplinary audience.
Advanced Fluorescence Reporters in Chemistry and Biology II
Advanced Fluorescence Reporters in Chemistry and Biology II - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Advanced Fluorescence Reporters in Chemistry and Biology II write by Alexander P. Demchenko. This book was released on 2010-09-08. Advanced Fluorescence Reporters in Chemistry and Biology II available in PDF, EPUB and Kindle. With contributions by numerous experts
Advanced Fluorescence Reporters in Chemistry and Biology I
Advanced Fluorescence Reporters in Chemistry and Biology I - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Advanced Fluorescence Reporters in Chemistry and Biology I write by Alexander P. Demchenko. This book was released on 2010-09-02. Advanced Fluorescence Reporters in Chemistry and Biology I available in PDF, EPUB and Kindle. Fluorescence reporter is the key element of any sensing or imaging technology. Its optimal choice and implementation is very important for increasing the sensitivity, precision, multiplexing power, and also the spectral, temporal, and spatial reso- tion in different methods of research and practical analysis. Therefore, design of ?uorescence reporters with advanced properties is one of the most important problems. In this volume, top experts in this ?eld provide advanced knowledge on the design and properties of ?uorescent dyes. Organic dyes were the ?rst ?uorescent materials used for analytical purposes, and we observe that they retain their leading positions against strong competition of new materials – conjugated polymers, semiconductor nanocrystals, and metal chelating complexes. Recently, molecular and cellular biology got a valuable tool of organic ?uorophores synt- sized by cell machinery and incorporated into green ?uorescent protein and its analogs. Demands of various ?uorescence techniques operating in spectral, anisotropy, and time domains require focused design of ?uorescence reporters well adapted to these techniques. Near-IR spectral range becomes more and more attractive for various applications, and new dyes emitting in this range are strongly requested. Two-photonic ?uorescence has become one of the major tools in bioimaging, and ?uorescence reporters well adapted to this technique are in urgent need. These problems cannot be solved without the knowledge of fundamental principles of dye design and of physical phenomena behind their ?uorescence response.
Advanced Fluorescence Reporters in Chemistry and Biology I
Advanced Fluorescence Reporters in Chemistry and Biology I - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Advanced Fluorescence Reporters in Chemistry and Biology I write by Alexander P. Demchenko. This book was released on 2010-11-04. Advanced Fluorescence Reporters in Chemistry and Biology I available in PDF, EPUB and Kindle. Fluorescence reporter is the key element of any sensing or imaging technology. Its optimal choice and implementation is very important for increasing the sensitivity, precision, multiplexing power, and also the spectral, temporal, and spatial reso- tion in different methods of research and practical analysis. Therefore, design of ?uorescence reporters with advanced properties is one of the most important problems. In this volume, top experts in this ?eld provide advanced knowledge on the design and properties of ?uorescent dyes. Organic dyes were the ?rst ?uorescent materials used for analytical purposes, and we observe that they retain their leading positions against strong competition of new materials – conjugated polymers, semiconductor nanocrystals, and metal chelating complexes. Recently, molecular and cellular biology got a valuable tool of organic ?uorophores synt- sized by cell machinery and incorporated into green ?uorescent protein and its analogs. Demands of various ?uorescence techniques operating in spectral, anisotropy, and time domains require focused design of ?uorescence reporters well adapted to these techniques. Near-IR spectral range becomes more and more attractive for various applications, and new dyes emitting in this range are strongly requested. Two-photonic ?uorescence has become one of the major tools in bioimaging, and ?uorescence reporters well adapted to this technique are in urgent need. These problems cannot be solved without the knowledge of fundamental principles of dye design and of physical phenomena behind their ?uorescence response.
Advanced Photon Counting
Advanced Photon Counting - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Advanced Photon Counting write by Peter Kapusta. This book was released on 2015-04-23. Advanced Photon Counting available in PDF, EPUB and Kindle. This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.
