Stimulated Raman Scattering Microscopy - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Stimulated Raman Scattering Microscopy write by Ji-Xin Cheng. This book was released on 2021-12-04. Stimulated Raman Scattering Microscopy available in PDF, EPUB and Kindle. Stimulated Raman Scattering Microscopy: Techniques and Applications describes innovations in instrumentation, data science, chemical probe development, and various applications enabled by a state-of-the-art stimulated Raman scattering (SRS) microscope. Beginning by introducing the history of SRS, this book is composed of seven parts in depth including instrumentation strategies that have pushed the physical limits of SRS microscopy, vibrational probes (which increased the SRS imaging functionality), data science methods, and recent efforts in miniaturization. This rapidly growing field needs a comprehensive resource that brings together the current knowledge on the topic, and this book does just that. Researchers who need to know the requirements for all aspects of the instrumentation as well as the requirements of different imaging applications (such as different types of biological tissue) will benefit enormously from the examples of successful demonstrations of SRS imaging in the book. Led by Editor-in-Chief Ji-Xin Cheng, a pioneer in coherent Raman scattering microscopy, the editorial team has brought together various experts on each aspect of SRS imaging from around the world to provide an authoritative guide to this increasingly important imaging technique. This book is a comprehensive reference for researchers, faculty, postdoctoral researchers, and engineers. - Includes every aspect from theoretic reviews of SRS spectroscopy to innovations in instrumentation and current applications of SRS microscopy - Provides copious visual elements that illustrate key information, such as SRS images of various biological samples and instrument diagrams and schematics - Edited by leading experts of SRS microscopy, with each chapter written by experts in their given topics
Advancements in Stimulated Raman Scattering Microscopy
Advancements in Stimulated Raman Scattering Microscopy - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Advancements in Stimulated Raman Scattering Microscopy write by Benjamin Figueroa (Jr.). This book was released on 2020. Advancements in Stimulated Raman Scattering Microscopy available in PDF, EPUB and Kindle. Stimulated Raman scattering (SRS) microscopy has transformed our ability to image chemical and biological systems because of its sub-micrometer resolution and molecular specificity. However, limitations in small spectral coverage, poor spectral resolution, and low sensitivity hamper the ability of SRS to investigate complex systems. Furthermore, the usage of SRS microscopy beyond biological systems is still very limited. Here I present my work which specifically addresses these issues. In chapters 2 and 3 I demonstrate the benefits of using SRS microscopy outside of its current biological niche, by investigating materials such as latent fingerprints and pharmaceutical tablets. In chapter 4 I address the limitations of poor spectral resolution and small spectral coverage by developing a parabolic fiber amplifier to provide a better SRS microscope. In chapter 5 I demonstrate an application of the developed fiber amplifier system for monitoring and quantifying temperature at the microscale level. Lastly, in chapter 6 I introduce the development of a new optical parametric oscillator (OPO) source to address the final limitation by increasing the sensitivity of SRS imaging, which ties into the future outlook on advancements needed in SRS microscopy.
Advances in Stimulated Raman Scattering Microscopy Via Deep Learning
Advances in Stimulated Raman Scattering Microscopy Via Deep Learning - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Advances in Stimulated Raman Scattering Microscopy Via Deep Learning write by Bryce Adrian Manifold. This book was released on 2022. Advances in Stimulated Raman Scattering Microscopy Via Deep Learning available in PDF, EPUB and Kindle. Stimulated Raman scattering (SRS) microscopy is a powerful chemical imaging technique that acquires images based on the vibrational-spectral "fingerprints" of molecules within an imaged field of view often without the need for exogenous fluorophores or labels. SRS microscopy has found an established niche in biophotonics with many examples of translational clinical applications and demonstrations of imaging various biological systems on subcellular to tissue spatial orders. Concurrent to the development of SRS microscopy, computational advancements have seen a democratized adoption of deep learning platforms for a wide variety of computer vision tasks. In this work I document my contributions in integrating SRS microscopy and deep learning towards advancing the capability to study biological systems. Specifically, deep learning will be shown to address technical limitations of SRS microscopy such as imaging noise and ultimate imaging depth in tissue samples. Deep learning will also be shown to improve analysis of SRS images via the development of a novel convolutional neural network architecture designed to handle a variety of chemical imaging techniques and perform a variety of computer vision tasks. Finally, I will show how these advancements and novel architecture can be used to diagnose thyroid cancer in label-free human tissue samples and to classify and study T cells based on label-free images.
Coherent Raman Scattering Microscopy
Coherent Raman Scattering Microscopy - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Coherent Raman Scattering Microscopy write by Ji-Xin Cheng. This book was released on 2016-04-19. Coherent Raman Scattering Microscopy available in PDF, EPUB and Kindle. The First Book on CRS MicroscopyCompared to conventional Raman microscopy, coherent Raman scattering (CRS) allows label-free imaging of living cells and tissues at video rate by enhancing the weak Raman signal through nonlinear excitation. Edited by pioneers in the field and with contributions from a distinguished team of experts, Coherent Raman Sc
Raman Microscopy
Raman Microscopy - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Raman Microscopy write by George Turrell. This book was released on 1996-06-24. Raman Microscopy available in PDF, EPUB and Kindle. One of the first books devoted entirely to the subject of Raman microscopy, Raman Microscopy addresses issues of great interest to engineers working in Raman-microscope development and researchers concerned with areas ofapplication for this science. The book is written by several world recognized experts, who summarize the Raman effect before discussing the hardware and software involved in todays instruments. This format provides an excellent introduction to this up-and-coming discipline. All important applications, including those in materials science and earth science are covered in depth. - Includes extensive description of the instrumentation, the Raman microspectrograph, the treatment of data, and micro-Raman imaging - Examines the use of Raman microscopy in diverse applications, including some of the hyphenated methods - Summarizes the Raman effect - Discusses new uses for this technology
