Affinity Chromatography - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Affinity Chromatography write by Pascal Bailon. This book was released on 2008-02-04. Affinity Chromatography available in PDF, EPUB and Kindle. Affinity chromatography, with its exquisite specificity, is based upon molecular recognition. It is a powerful tool for the purification of biomolecules. In recent years, numerous new applications and modified techniques have been derived from gro- specific interactions and biological recognition principles. An up-to-date review of the past, current, and future applications of affinity chromatography has been presented in the introductory chapter by Meir Wilchek and Irwin Chaiken. Though many of these new applications and techniques are well documented in the literature, it is often difficult to find methods that are written with the intent of helping new practitioners of affinity chromatography. This volume on Affinity Chromatography: Methods and Protocols is intended for the novice, as well as for - perts in the field. The protocols are written by experts who have developed and/or successfully employed these methods in their laboratories. Each chapter describes a specific technique, and since the book is intended to help the beginner, each technique is described simply and clearly, making sure that all relevant steps are included, assuming no previous knowledge. Each chapter contains an introduction describing the principles involved, followed by a Materials and Methods section, which lays the groundwork for the reader to conduct experiments step-by-step, in an orderly fashion. The following Notes section, which describes many of the problems that can occur, makes suggestions for overcoming them, and provides alternate procedures. These are precisely the sort of important, practical details that never seem to appear in the published literature.
Affinity Chromatography
Affinity Chromatography - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Affinity Chromatography write by J. Turková. This book was released on 1978-01-01. Affinity Chromatography available in PDF, EPUB and Kindle. Bioaffinity chromatography has increasingly become the method of choice for the purification, determination or removal of many biologically active substances.
Handbook of Affinity Chromatography
Handbook of Affinity Chromatography - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Handbook of Affinity Chromatography write by David S. Hage. This book was released on 2005-07-19. Handbook of Affinity Chromatography available in PDF, EPUB and Kindle. This essential handbook guides investigators in the theory, applications, and practical use of affinity chromatography in a variety of fields including biotechnology, biochemistry, molecular biology, analytical chemistry, proteomics, pharmaceutical science, environmental analysis, and clinical chemistry. The Handbook of Affinity Chromatograph
Process Scale Purification of Antibodies
Process Scale Purification of Antibodies - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Process Scale Purification of Antibodies write by Uwe Gottschalk. This book was released on 2017-03-07. Process Scale Purification of Antibodies available in PDF, EPUB and Kindle. Promoting a continued and much-needed renaissance in biopharmaceutical manufacturing, this book covers the different strategies and assembles top-tier technology experts to address the challenges of antibody purification. • Updates existing topics and adds new ones that include purification of antibodies produced in novel production systems, novel separation technologies, novel antibody formats and alternative scaffolds, and strategies for ton-scale manufacturing • Presents new and updated discussions of different purification technologies, focusing on how they can address the capacity crunch in antibody purification • Emphasizes antibodies and innovative chromatography methods for processing
Immunological Methods
Immunological Methods - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Immunological Methods write by Ivan Lefkovits. This book was released on 2014-06-28. Immunological Methods available in PDF, EPUB and Kindle. Immunological Methods a compendium of basic research techniques being used in one of the largest immunology research institutes, the Basel Institute for Immunology, with particular emphasis given to new methodology. The procedures have been described by individuals judged to be highly expert in their specialties. In many instances the methods developed or adapted to unique uses by the contributors have not previously been described in detail. The book contains 34 chapters covering techniques for detection, isolation, and purification of antibodies (including dansylation, two-dimensional chromatography, isoelectric focusing, polyacrylamide gel electrophoresis, and isotachophoresis); measurement of equilibrium constants (equilibrium dialysis, filtration, and sedimentation); and isotope and fluorescent labeling and detection of cell-surface components. Techniques such as isotope laboratory maintenance; chemical modification of proteins, haptens, and solid supports, and haptenation of viable biological carriers; production of antisera against allotypes and histocompatibility antigens and production of antibody with clonai dominance; histocompatibility and MLR testing; and cell separation by haptenated gels and by velocity sedimentation of rosette-forming cells are also discussed. Other chapters cover detection of antibody-secreting and alloantigen-binding cells; immune responses in vitro and their analysis by limiting dilution; production of T-cell factors; hybridoma production by cell fusion; maintenance of cell lines and cloning in semisolid media; and the mathematical analysis of immunological data.
