Artificial Nucleases - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Artificial Nucleases write by Marina A. Zenkova. This book was released on 2012-12-06. Artificial Nucleases available in PDF, EPUB and Kindle. The development of agents capable of cleaving RNA and DNA has attracted considerable attention from researchers in the last few years, because of the immediate and very important applications they can find in the emerging fields of biotechnology and pharmacology. There are essentially two classes of these agents - nucleases that occur naturally inside cells and synthetically produced artificial nucleases. The first class includes protein enzyme nucle ases and catalytic RNA structured ribozymes that perform cleavage of the phosphodiester bonds in nucleic acids according to a hydrolytic pathway in the course of different biochemical processes in the cell. A different pathway is used by some antibiotics which cleave DNA via redox-based mechanisms resulting in oxidative damage of nucleotide units and breakage of the DNA backbone. The above molecules are indispensable tools for manipulating nucleic acids and processing RNA; DNA-cleaving antibiotics and cytotoxic ribonucleases have demonstrated utility as chemotherapeutic agents. The second class, artificial nucleases, are rationally designed to imitate the active centers of natural enzymes by simple structures possessing minimal sets of the most important characteristics that are essential for catalysis. A dif ferent approach, in vitro selection, was also used to create artificial RNA and DNA enzymes capable of cleaving RNA. Being less efficient and specific as compared to the natural enzymes, the primitive mimics are smaller and robust and can function in a broad range of conditions.
Artificial Nucleases
Artificial Nucleases - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Artificial Nucleases write by Marina A. Zenkova. This book was released on 2004-01-07. Artificial Nucleases available in PDF, EPUB and Kindle. The development of agents capable of cleaving RNA and DNA has attracted considerable attention from researchers in the last few years, because of the immediate and very important applications they can find in the emerging fields of biotechnology and pharmacology. There are essentially two classes of these agents - nucleases that occur naturally inside cells and synthetically produced artificial nucleases. The first class includes protein enzyme nucle ases and catalytic RNA structured ribozymes that perform cleavage of the phosphodiester bonds in nucleic acids according to a hydrolytic pathway in the course of different biochemical processes in the cell. A different pathway is used by some antibiotics which cleave DNA via redox-based mechanisms resulting in oxidative damage of nucleotide units and breakage of the DNA backbone. The above molecules are indispensable tools for manipulating nucleic acids and processing RNA; DNA-cleaving antibiotics and cytotoxic ribonucleases have demonstrated utility as chemotherapeutic agents. The second class, artificial nucleases, are rationally designed to imitate the active centers of natural enzymes by simple structures possessing minimal sets of the most important characteristics that are essential for catalysis. A dif ferent approach, in vitro selection, was also used to create artificial RNA and DNA enzymes capable of cleaving RNA. Being less efficient and specific as compared to the natural enzymes, the primitive mimics are smaller and robust and can function in a broad range of conditions.
Molecular Biology of Nucleases
Molecular Biology of Nucleases - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Molecular Biology of Nucleases write by Nawin C. Mishra. This book was released on 1995-03-06. Molecular Biology of Nucleases available in PDF, EPUB and Kindle. Nucleases occupy a central position in the biochemistry of DNA transactions and other metabolism of nucleic acids in all organisms. They have also proven useful in modern biological studies crucial for the development of recombinant DNA technology and reverse genetics. Nucleases assist in the identification and characterization of genes responsible for several diseases and their possible alleviation by gene therapy. Molecular Biology of Nucleases introduces the properties and biological roles of nucleases. It is the one comprehensive source for newcomers to the field.
Artificial Enzymes
Artificial Enzymes - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Artificial Enzymes write by Ronald Breslow. This book was released on 2006-05-12. Artificial Enzymes available in PDF, EPUB and Kindle. This first book on this fascinating topic is edited by one of today's most famous and internationally respected organic chemists, renowned for his pioneering synthesis of the cyclopropenyl cation. For his part, Ronald Breslow has brought together leading scientists in this expanding area to provide a novel overview of protein-, cyclodextrin-, metal- and porphyrin-based artificial enzymes as well as enzyme-like polymers and dendrimers. A must for all scientists interested in this emerging field.
Functional Genomics and Biotechnology in Solanaceae and Cucurbitaceae Crops
Functional Genomics and Biotechnology in Solanaceae and Cucurbitaceae Crops - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Functional Genomics and Biotechnology in Solanaceae and Cucurbitaceae Crops write by Hiroshi Ezura. This book was released on 2016-01-04. Functional Genomics and Biotechnology in Solanaceae and Cucurbitaceae Crops available in PDF, EPUB and Kindle. This volume summarizes recent technological advances in the design and engineering of Solanaceae and Cucurbitaceae crops. It begins with contributions on the tomato and melon genome sequence, databases for Solanaceae and Cucurbitaceae research, DNA markers in the breeding of the two families, and mutant resources and TILLING platforms in tomato research. Subsequent chapters address the use of molecular techniques for the modification of important breeding traits, such as tomato fruit set, growth, ripening, and sugar accumulation, as well as disease and insect resistance in melons. The volume closes with chapters on genome editing using artificial nucleases as a future breeding tool, and on the development of an in silico crop design system. It offers a valuable resource for plant breeders, molecular biologists, and agronomists.
