Biosynthesis of a Fully Functional Cyclotide Inside Living Bacterial Cells - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Biosynthesis of a Fully Functional Cyclotide Inside Living Bacterial Cells write by . This book was released on 2007. Biosynthesis of a Fully Functional Cyclotide Inside Living Bacterial Cells available in PDF, EPUB and Kindle. The cyclotide MCoTI-II is a powerful trypsin inhibitor recently isolated from the seeds of Momordica cochinchinensis, a plant member of cucurbitaceae family. We report for the first time the in vivo biosynthesis of natively-folded MCoTI-II inside live E. coli cells. Our biomimetic approach involves the intracellular backbone cyclization of a linear cyclotide-intein fusion precursor mediated by a modified protein splicing domain. The cyclized peptide then spontaneously folds into its native conformation. The use of genetically engineered E. coli cells containing mutations in the glutathione and thioredoxin reductase genes considerably improves the production of folded MCoTI-II in vivo. Biochemical and structural characterization of the recombinant MCoTI-II confirmed its identity. Biosynthetic access to correctly-folded cyclotides allows the possibility of generating cell-based combinatorial libraries that can be screened inside living cells for their ability to modulate or inhibit cellular processes.
Plant Cyclotides
Plant Cyclotides - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Plant Cyclotides write by . This book was released on 2015-11-24. Plant Cyclotides available in PDF, EPUB and Kindle. Advances in Botanical Research publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences. Currently in its 76th volume, the series features several reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology and ecology. Publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences Contains commentary by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology, and ecology This volume features reviews of the fast moving field of plant cyclotides
Microbial Production of High-Value Products
Microbial Production of High-Value Products - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Microbial Production of High-Value Products write by Bernd H. A. Rehm. This book was released on 2022-09-06. Microbial Production of High-Value Products available in PDF, EPUB and Kindle. This edited volume emphasizes how microorganisms have become a reliable pillar of biotechnology. The authors discuss advances in synthetic biology and genetic engineering that have made it possible to reprogram the microbial cellular capabilities. This enables an economically viable production of high-value products at an industrial level. The first part of the book provides an overview of synthetic biology and genome editing tools for engineering microbial cell factories in modern fermentation. Readers also learn how high-throughput bioprocessing methods are used to recover and purify microbial products. The remaining parts of this book explore the implementation and challenges of these upstream and downstream processing techniques for manufacturing high-value products. Cost-effectiveness and quality-control are key factors, when discussing the production of low-molecular-weight products, biopharmaceuticals, biopolymers and protein-based nanoparticles. This book is a valuable resource for biotechnologists both in the industry and in academia.
Protein Engineering Handbook
Protein Engineering Handbook - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Protein Engineering Handbook write by Stefan Lutz. This book was released on 2012-07-31. Protein Engineering Handbook available in PDF, EPUB and Kindle. Unparalleled in size and scope, this new major reference integrates academic and industrial knowledge into a single resource, allowing for a unique overview of the entire field. Adopting a systematic and practice-oriented approach, and including a wide range of technical and methodological information, this highly accessible handbook is an invaluable 'toolbox' for any bioengineer. In two massive volumes, it covers the full spectrum of current concepts, methods and application areas.
Folding of Disulfide Proteins
Folding of Disulfide Proteins - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Folding of Disulfide Proteins write by Rowen J. Y. Chang. This book was released on 2011-08-12. Folding of Disulfide Proteins available in PDF, EPUB and Kindle. This book aims to cover the knowledge of protein folding accumulated from studies of disulfide-containing proteins, including methodologies, folding pathways, and folding mechanism of numerous extensively characterized disulfide proteins. Folding of Disulfide Proteins will be valuable supplementary reading for general biochemistry, biophysics, molecular biology, and cellular biology courses for graduate and undergraduate students. This book can also be used for specialized graduate-level biochemistry, biophysics, and molecular biology courses dedicated to protein folding as well as related biological problems and diseases. Will also be of interest to everybody interested in problems related to protein folding, and anyone who is interested in understanding the mechanism of protein misfolding and protein misfolding-related diseases.
