Plant Cyclotides - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Plant Cyclotides write by . This book was released on 2015-11-24. Plant Cyclotides available in PDF, EPUB and Kindle. Advances in Botanical Research publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences. Currently in its 76th volume, the series features several reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology and ecology. Publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences Contains commentary by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology, and ecology This volume features reviews of the fast moving field of plant cyclotides
Biosynthesis of Cyclotides
Biosynthesis of Cyclotides - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Biosynthesis of Cyclotides write by Cameron Victor Jennings. This book was released on 2002. Biosynthesis of Cyclotides available in PDF, EPUB and Kindle. Several members of the Rubiaceae, Violaceae and Cucurbitaceae produce a novel family of circular disulfide-rich polypeptides that have been called cyclotides. Cyclotides are typically about 30 amino acids in size, contain an N- to C- cyclised backbone and incorporate three disulfide bonds that are arranged in a cystine knot motif. The combination of this knotted and strongly braced structure with a circular backbone renders the cyclotides impervious to enzymatic breakdown and makes them exceptionally stable. The aim of this thesis was to understand the mechanism of synthesis of the unusual family of cyclic peptides. Species of plants used were Oldenlandia affinia, Arabidopsis thaliana, and Nicotiana tabacum. (abstract)
The Biosynthesis of Plant Cyclotides
The Biosynthesis of Plant Cyclotides - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook The Biosynthesis of Plant Cyclotides write by Amanda Diane Gillon. This book was released on 2007. The Biosynthesis of Plant Cyclotides available in PDF, EPUB and Kindle. A highly conserved tri-peptide motif at the C-terminus of the cyclotide domain was also found to be essential for cyclisation. This work has provided important insights into how circular proteins are synthesised in plants and has asssited with the elucidation of primary and secondary structual requirements for protein cyclisation.
Biosynthesis of the Cyclotide Kalata B1 Using a Protein Splicing Unit
Biosynthesis of the Cyclotide Kalata B1 Using a Protein Splicing Unit - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Biosynthesis of the Cyclotide Kalata B1 Using a Protein Splicing Unit write by . This book was released on 2005. Biosynthesis of the Cyclotide Kalata B1 Using a Protein Splicing Unit available in PDF, EPUB and Kindle. Cyclotides are a newly emerging family of large backbone cyclic polypeptides (≈30 residues long) characterized by a disulfide-stabilized core (3 disulfide bonds) with an unusual knotted structure. In contrast to other cyclic polypeptides, cyclotides have a well-defined three-dimensional structure. Therefore, despite their small size, they can be considered miniproteins. The unique cyclic-backbone topology and knotted arrangement of 3 disulfide bonds endow cyclotides with exceptional stability and resistance to chemical, enzymatic and thermal degradation. Furthermore, their well-defined structures have been associated with a range of biological functions. Together, these characteristics suggest that cyclotides are ideal molecular scaffolds for the development of stable peptide drugs. Despite the fact that the chemical synthesis of circular peptides has been well explored and a number different approaches involving solid-phase or liquid-phase exist, recent developments in the fields of molecular biology and protein engineering have now made possible the biosynthesis of cyclic peptides. This progress has been made mainly in two areas, non-ribosomal peptide synthesis and Expressed Protein Ligation (EPL)/protein trans-splicing. Access to biosynthetic cyclotides using recombinant DNA expression techniques offers the exciting possibility of producing large combinatorial libraries of highly stable miniproteins. This would allow the generation of cell-based combinatorial libraries that could be screened either in vitro or in vivo for their ability to regulate cellular processes. In the present work, we describe the biosynthesis of the cyclotide Kalata B1 (KB1) in E. coli using an engineered intein. Our approach (Figure 1) is based on an intramolecular version of Native Chemical Ligation (NCL). NCL involves the chemoselective reaction between a N-terminal Cys residue of one peptide and an [alpha]-thioester group of a second peptide. Importantly, incorporation of these two groups into the same synthetic polypeptide leads to efficient circularization.
Biosynthesis of the Cyclotide MCoTI-II Using an Engineered Intein
Biosynthesis of the Cyclotide MCoTI-II Using an Engineered Intein - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Biosynthesis of the Cyclotide MCoTI-II Using an Engineered Intein write by J. A. Camarero. This book was released on 2006. Biosynthesis of the Cyclotide MCoTI-II Using an Engineered Intein available in PDF, EPUB and Kindle. Cyclotides are an emerging family of naturally occurring circular mini-proteins ({approx}30-40 amino acids) characterized by six conserved Cys residues (forming 3 disulfide bridges) that create a topologically unique structure designated as a cyclic cysteine knot (CCK). The cysteine knot motif, which is embedded within the macrocylic backbone, is described as two disulfide bridges that form a ring that is penetrated by the third disulfide bridge. The cyclic backbone and CCK motif together confer cyclotides with a remarkable stability and resistance to proteolytic, chemical, and thermal degradation. Further, cyclotides are functionally diverse and display a wide range of functions including uterotonic activity, trypsin inhibition, cytotoxicity, neurotensin binding, anti-HIV, antimicrobial, and insecticidal activity. Together, these characteristics make cyclotides attractive candidates for both drug design and agricultural applications, both in their native forms and as molecular scaffolds for the incorporation of novel bioactivities. [1] The ability to manipulate production of cyclotides within biological systems is critical for mutagenesis studies, production of grafted products, and the mass production of cyclotides with novel activities. My adviser's hope is to achieve this capability by employing recombinant DNA expression techniques to generate large combinatorial libraries of cyclotides. The advantage in creating a biosynthetic library (containing {approx}10{sup 6}-10{sup 10} members/library vs. chemically based libraries with typical values ranging from {approx}10{sup 3}-10{sup 5} members/library) is that it can be lead to the in vivo application of biological screening and selection methodologies based on a specific clone's ability to affect certain cellular processes.
