Cellular and Molecular Methods in Neuroscience Research - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Cellular and Molecular Methods in Neuroscience Research write by Adalberto Merighi. This book was released on 2007-12-14. Cellular and Molecular Methods in Neuroscience Research available in PDF, EPUB and Kindle. There are numerous books on cellular and molecular protocols for general use in cell biology but very few are exclusively devoted to neurobiology. This book fills this gap and explains in a clear and consistent manner, some of the more commonly used protocols in neuroscience research. Each chapter is written by either the person who invented the procedure or an expert in the field. The format is uniform: "Overview," "Background," "Protocols," and "results and discussion." Each protocol begins with the principle of the technique, studies in cell culture, materials and reagents, and, lastly, step-by-step outline of the procedure itself. This highly practical book is also well illustrated (with 17 four color plates) to make the concepts and procedures easy to understand and perform.
Molecular Neuroscience
Molecular Neuroscience - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Molecular Neuroscience write by Rusty Lansford. This book was released on 2014. Molecular Neuroscience available in PDF, EPUB and Kindle. A wide variety of powerful molecular techniques have been applied to biology in recent decades, ranging from recombinant DNA technologies to state-of-the-art imaging methods. But the plethora of techniques available combined with the complexities of neurobiological systems can make it difficult for neuroscientists to select and carry out an experimental procedure to effectively address the question at hand. This laboratory manual serves as a comprehensive practical guide to molecular and cellular methods for neuroscientists. It consists of five major sections: Working with Cells, Working with DNA, Working with RNA, Gene Transfer, and Imaging. Each includes step-by-step protocols and discussions of basic and cutting-edge procedures for working in that area. Fundamental techniques include maintaining a sterile working environment, purifying and culturing neural cells, isolating and manipulating DNA and RNA, and understanding and using a microscope. Advanced topics include single-neuron isolation and analysis, in vivo gene delivery and imaging, optogenetics, RNA interference, transgenic technologies, high-throughput analysis of gene expression (e.g., RNA-Seq), and constructing and imaging fluorescent proteins. The manual includes protocols developed in the Advanced Techniques in Molecular Neuroscience course offered annually at Cold Spring Harbor Laboratory, as well as protocols drawn from its best-selling lab manuals. It is an essential resource for all neuroscientists, from graduate students upward, who seek to use molecular techniques to probe the complexities of the nervous system.
Cellular Imaging Techniques for Neuroscience and Beyond
Cellular Imaging Techniques for Neuroscience and Beyond - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Cellular Imaging Techniques for Neuroscience and Beyond write by Floris G. Wouterlood. This book was released on 2012-08-08. Cellular Imaging Techniques for Neuroscience and Beyond available in PDF, EPUB and Kindle. In the biomedical sciences, the confocal laser scanning microscope (CLSM) has become the instrument of choice for producing high-resolution images and 3D reconstruction, breaking the barriers of conventional optical microscopy. Wouterlood (anatomy, VU U. Medical Center, Amsterdam, the Netherlands) introduces the confocal principle which eliminates out-of-focus haze, its components, and relevant equations. International scientists explain the principles and related methods of stimulated emission depletion (SRED), single molecule localization, and coherent anti-Stokes Raman (CARS) microscopy; labeling approaches; preparation of samples for imaging; and applications of, and developments in, this new wave of imaging, e.g., visualization of neuronal networks, DNA, and myelin. The text includes color and b&w images, and referral to an online CLSM simulator. Academic Press is an imprint of Elsevier. Annotation ©2013 Book News, Inc., Portland, OR (booknews.com).
From Molecules to Networks
From Molecules to Networks - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook From Molecules to Networks write by Ruth Heidelberger. This book was released on 2009-01-27. From Molecules to Networks available in PDF, EPUB and Kindle. An understanding of the nervous system at virtually any level of analysis requires an understanding of its basic building block, the neuron. From Molecules to Networks provides the solid foundation of the morphologic, biochemical, and biophysical properties of nerve cells. All chapters have been thoroughly revised for this second edition to reflect the significant advances of the past 5 years. The new edition expands on the network aspects of cellular neurobiology by adding a new chapter, Information Processing in Neural Networks, and on the relation of cell biological processes to various neurological diseases. The new concluding chapter illustrates how the great strides in understanding the biochemical and biophysical properties of nerve cells have led to fundamental insights into important aspects of neurodegenerative disease. Written and edited by leading experts in the field, the second edition completely and comprehensively updates all chapters of this unique textbook Discusses emerging new understanding of non-classical molecules that affect neuronal signaling Full colour, professional graphics throughout Includes two new chapters: Information Processing in Neural Networks - describes the principles of operation of neural networks and the key circuit motifs that are common to many networks in the nervous system. Molecular and Cellular Mechanisms of Neurodegenerative Disease - introduces the progress made in the last 20 years in elucidating the cellular and molecular mechanisms underlying brain disorders, including Amyotrophic Lateral Sclerosis (ALS), Parkinson disease, and Alzheimer’s disease
Guide to Research Techniques in Neuroscience
Guide to Research Techniques in Neuroscience - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Guide to Research Techniques in Neuroscience write by Matt Carter. This book was released on 2022-03-26. Guide to Research Techniques in Neuroscience available in PDF, EPUB and Kindle. Modern neuroscience research is inherently multidisciplinary, with a wide variety of cutting edge new techniques to explore multiple levels of investigation. This Third Edition of Guide to Research Techniques in Neuroscience provides a comprehensive overview of classical and cutting edge methods including their utility, limitations, and how data are presented in the literature. This book can be used as an introduction to neuroscience techniques for anyone new to the field or as a reference for any neuroscientist while reading papers or attending talks. Nearly 200 updated full-color illustrations to clearly convey the theory and practice of neuroscience methods Expands on techniques from previous editions and covers many new techniques including in vivo calcium imaging, fiber photometry, RNA-Seq, brain spheroids, CRISPR-Cas9 genome editing, and more Clear, straightforward explanations of each technique for anyone new to the field A broad scope of methods, from noninvasive brain imaging in human subjects, to electrophysiology in animal models, to recombinant DNA technology in test tubes, to transfection of neurons in cell culture Detailed recommendations on where to find protocols and other resources for specific techniques "Walk-through" boxes that guide readers through experiments step-by-step
