Characterization of Protein Therapeutics using Mass Spectrometry - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Characterization of Protein Therapeutics using Mass Spectrometry write by Guodong Chen. This book was released on 2014-07-08. Characterization of Protein Therapeutics using Mass Spectrometry available in PDF, EPUB and Kindle. This book highlights current approaches and future trends in the use of mass spectrometry to characterize protein therapies. As one of the most frequently utilized analytical techniques in pharmaceutical research and development, mass spectrometry has been widely used in the characterization of protein therapeutics due to its analytical sensitivity, selectivity, and specificity. This book begins with an overview of mass spectrometry techniques as related to the analysis of protein therapeutics, structural identification strategies, quantitative approaches, followed by studies involving characterization of process related protein drug impurities/degradants, metabolites, higher order structures of protein therapeutics. Both general practitioners in pharmaceutical research and specialists in analytical sciences will benefit from this book that details step-by-step approaches and new strategies to solve challenging problems related to protein therapeutics research and development.
Protein Analysis using Mass Spectrometry
Protein Analysis using Mass Spectrometry - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Protein Analysis using Mass Spectrometry write by Mike S. Lee. This book was released on 2017-05-26. Protein Analysis using Mass Spectrometry available in PDF, EPUB and Kindle. Presents Practical Applications of Mass Spectrometry for Protein Analysis and Covers Their Impact on Accelerating Drug Discovery and Development Covers both qualitative and quantitative aspects of Mass Spectrometry protein analysis in drug discovery Principles, Instrumentation, Technologies topics include MS of peptides, proteins, and ADCs , instrumentation in protein analysis, nanospray technology in MS protein analysis, and automation in MS protein analysis Details emerging areas from drug monitoring to patient care such as Identification and validation of biomarkers for cancer, targeted MS approaches for biomarker validation, biomarker discovery, and regulatory perspectives Brings together the most current advances in the mass spectrometry technology and related method in protein analysis
Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics
Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics write by Allan Matte. This book was released on 2020-09-07. Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics available in PDF, EPUB and Kindle. Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics provides the interested and informed reader with an overview of current approaches, strategies and considerations relating to the purification, analytics and characterization of therapeutic antibodies and related molecules. While there are obviously other books published in and around this subject area, they seem to be either older (c.a. year 2000 publication date) or are more limited in scope. The book will include an extensive bibliography of the published literature in the respective areas covered. It is not, however, intended to be a how-to methods book. Covers the vital new area of R&D on therapeutic antibodies Written by leading scientists and researchers Up-to-date coverage and includes a detailed bibliography
Characterization of Protein-Protein Interactions for Therapeutic Drug Design Utilizing Mass Spectrometry
Characterization of Protein-Protein Interactions for Therapeutic Drug Design Utilizing Mass Spectrometry - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Characterization of Protein-Protein Interactions for Therapeutic Drug Design Utilizing Mass Spectrometry write by Alex J. Johnson. This book was released on 2015. Characterization of Protein-Protein Interactions for Therapeutic Drug Design Utilizing Mass Spectrometry available in PDF, EPUB and Kindle. The number of transferrin based therapeutics progressing to clinical trials remains disappointingly small despite promising capabilities of transporting therapeutic payloads to cancer cells and across the blood brain barrier. This meager success record is largely due to the complexity and heterogeneity of all protein conjugation products that generates difficulties for their analytical characterization. Discussed in this work, transferrin is conjugated to lysozyme as a model therapeutic to deliver this bacteriostatic protein to target central nervous system infections. In this work ESI- and MALDI-MS were used to characterize the modification sites at lysine residues in hopes of characterizing heterogeneity within the conjugate. Identification and quantization of modification sites using MS on tryptic digested samples proved difficult with poor signal to noise ratios and missing peptide fragments. The use of an 18O labeling method that exchanges both C-terminal oxygen atoms with 18O provided more reliable results, but still proved difficult to observe all needed peptide fragments. MALDI-MS allowed for verification of ESI-MS results, but was found unhelpful with full characterization due to abundant overlapping of isotopic labeled peaks. Hoping to create an ideal 1:1 binding ratio between the two proteins, a site-specific modification method using kinetically controlled conditions was used and was confirmed that the method, although capable of producing 1:1 conjugated species, actually created different isomers with separate binding frequencies at each lysine. Online-IEC helped with the identification of isomers and started the initial work of correlating modification sites with bioactivity of the proteins. It was determined that lysozyme has a high chance of being modified at lysine 33 and 116, with a possibility of also being highly modified at lysine 97. More work is needed to complete the characterization, especially with transferrin, but the experimental approaches developed in this work prove to be promising. This work aims at delivering an optimized framework for analytical characterization of protein and antibody conjugates to guide the development of future biopharmaceuticals.
Protein and Peptide Mass Spectrometry in Drug Discovery
Protein and Peptide Mass Spectrometry in Drug Discovery - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Protein and Peptide Mass Spectrometry in Drug Discovery write by Michael L. Gross. This book was released on 2011-09-26. Protein and Peptide Mass Spectrometry in Drug Discovery available in PDF, EPUB and Kindle. The book that highlights mass spectrometry and its application in characterizing proteins and peptides in drug discovery An instrumental analytical method for quantifying the mass and characterization of various samples from small molecules to large proteins, mass spectrometry (MS) has become one of the most widely used techniques for studying proteins and peptides over the last decade. Bringing together the work of experts in academia and industry, Protein and Peptide Mass Spectrometry in Drug Discovery highlights current analytical approaches, industry practices, and modern strategies for the characterization of both peptides and proteins in drug discovery. Illustrating the critical role MS technology plays in characterizing target proteins and protein products, the methods used, ion mobility, and the use of microwave radiation to speed proteolysis, the book also covers important emerging applications for neuroproteomics and antigenic peptides. Placing an emphasis on the pharmaceutical industry, the book stresses practice and applications, presenting real-world examples covering the most recent advances in mass spectrometry, and providing an invaluable resource for pharmaceutical scientists in industry and academia, analytical and bioanalytical chemists, and researchers in protein science and proteomics.
