Circulating MicroRNA Profiling in Cancer Biomarker Discovery - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Circulating MicroRNA Profiling in Cancer Biomarker Discovery write by Francesca Scionti. This book was released on 2018. Circulating MicroRNA Profiling in Cancer Biomarker Discovery available in PDF, EPUB and Kindle. MicroRNAs (miRNAs) are a class of small non-coding RNA molecules of approximately 22 nucleotides that regulate gene expression at the post-transcriptional level. Alterations in miRNA expression patterns correlate with a wide spectrum of pathological conditions, including cancer. miRNA profiling was mostly performed, in solid tissues, obtained by invasive diagnostic procedures. However, miRNAs in biofluids, such as serum and plasma, show high stability resulting from the formation of complexes with specific protein or incorporation within circulating exosomes or other microvesicles. Circulating miRNAs could be reliable biomarkers for early-stage cancer diagnosis, prognosis and response to therapy. In this chapter, we analyze the major pre-analytical and analytical challenges in experimental design for circulating miRNA detection, focusing on exosome fraction and microarray-based approach.
Circulating microRNAs in Disease Diagnostics and their Potential Biological Relevance
Circulating microRNAs in Disease Diagnostics and their Potential Biological Relevance - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Circulating microRNAs in Disease Diagnostics and their Potential Biological Relevance write by Peter Igaz. This book was released on 2015-11-25. Circulating microRNAs in Disease Diagnostics and their Potential Biological Relevance available in PDF, EPUB and Kindle. MicroRNAs as the endogenous mediators of RNA interference have experienced an unprecedented career in recent years, highlighting their pathogenic, diagnostic and potential therapeutic relevance. Beside tissue microRNAs, they are also found in body fluids, most notably in blood. Significant differences of circulating microRNA levels have been found in various diseases, making them candidates for minimally invasive markers of disease, for example tumor malignancy. The book focuses on the potential diagnostic applicability of circulating microRNAs in various diseases and their potential biological significance.
Genomic Biomarkers for Pharmaceutical Development
Genomic Biomarkers for Pharmaceutical Development - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Genomic Biomarkers for Pharmaceutical Development write by Elizabeth Mambo. This book was released on 2013-07-16. Genomic Biomarkers for Pharmaceutical Development available in PDF, EPUB and Kindle. The focus of this chapter is on the development of miRNA diagnostics and the process for miRNA biomarker development. We discuss pancreatic cancer as a model for the application of miRNA-based tests in different clinical biopsy specimen types. Asuragen’s miRInform® Pancreas test illustrates the development of a laboratory developed test (LDT) that was validated in compliance with Clinical Laboratory Improvement Amendments (CLIA) and College of American Pathologists (CAP) regulations. We provide an overview of work on miRNA-based signatures which is focused on resolving clinical dilemmas in solid and cystic pancreatic lesions. We also review some of the promising miRNA candidates identified in biofluids from other cancer types. These studies highlight the emergence of miRNA-based diagnostic tools which have the potential to impact the clinical management of cancer by guiding early diagnosis, aiding in the assessment of patients’ prognosis, and enabling the identification of novel, more effective therapeutic targets.
The origin, function and diagnostic potential of extracellular microRNA in human body fluids
The origin, function and diagnostic potential of extracellular microRNA in human body fluids - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook The origin, function and diagnostic potential of extracellular microRNA in human body fluids write by Andrey Turchinovich. This book was released on 2014-07-28. The origin, function and diagnostic potential of extracellular microRNA in human body fluids available in PDF, EPUB and Kindle. Short non-coding RNA molecules, microRNAs (miRNAs), post-transcriptionally regulate gene expression in living cells. In recent years, miRNAs have been found in a wide spectrum of mammalian body fluids including blood plasma, saliva, urine, milk, seminal plasma, tears and amniotic fluid as extracellular circulating nuclease-resistant entities. The changes in miRNA spectra observed in certain fluids correlated with various pathological conditions suggesting that extracellular miRNAs can serve as informative biomarkers for certain diseases including cancer. However, the mechanism of generation and a biological role of extracellular miRNAs remain unclear. The current theories regarding extracellular miRNA origin and function suggest that these miRNAs can be either non-specific ‘by-products’ of cellular activity and cell death or specifically released cell-cell signaling messengers. The goal of this Research Topic is to bring together up-to-date knowledge about the extracellular miRNA and its role in disease diagnostics and, possibly, inter-cellular communication.
MicroRNA as Biomarkers in Cancer Diagnostics and Therapy
MicroRNA as Biomarkers in Cancer Diagnostics and Therapy - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook MicroRNA as Biomarkers in Cancer Diagnostics and Therapy write by Lorenzo F. Sempere. This book was released on 2019-07-30. MicroRNA as Biomarkers in Cancer Diagnostics and Therapy available in PDF, EPUB and Kindle. This book is a printed edition of the Special Issue MicroRNA as Biomarkers in Cancer Diagnostics and Therapy that was published in IJMS
