Computer Simulations of Aggregation of Proteins and Peptides - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Computer Simulations of Aggregation of Proteins and Peptides write by Mai Suan Li. This book was released on 2022-02-16. Computer Simulations of Aggregation of Proteins and Peptides available in PDF, EPUB and Kindle. This volume provides computational methods and reviews various aspects of computational studies of protein aggregation. Chapters discuss the relationship between protein misfolding and protein aggregation, methods of prediction of aggregation propensities of protein, peptides, protein structure, results of computer simulations of aggregation, and computational simulations focused on specific diseases such as Alzheimer’s, Parkinson’s, and preeclampsia. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Computer Simulations of Aggregation of Proteins and Peptides aims to ensure successful results in the further study of this vital field.
Protein Folding, Misfolding and Aggregation
Protein Folding, Misfolding and Aggregation - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Protein Folding, Misfolding and Aggregation write by Victor Muñoz. This book was released on 2008-06-24. Protein Folding, Misfolding and Aggregation available in PDF, EPUB and Kindle. Protein folding and aggregation is the process by which newly synthesized proteins fold into the specific three-dimensional structures defining their biologically active states. It has always been a major focus of research in biochemistry and has often been seen as the unsolved second part of the genetic code. In the last 10 years we have witnessed a quantum leap in the research in this exciting area. Computational methods have improved to the extent of making possible to simulate the complete folding process of small proteins and the early stages of protein aggregation. Experimental methods have evolved to permit resolving fast processes of folding reactions and visualizing single molecules during folding. The findings from these novel experiments and detailed computer simulations have confirmed the main predictions of analytical theory of protein folding. In summary, protein folding research has finally acquired the status of a truly quantitative science, paving the way for more exciting developments in the near future. This unique book covers all the modern approaches and the many advances experienced in the field during the last 10 years. There is also much emphasis on computational methods and studies of protein aggregation which have really flourished in the last decade. It includes chapters in the areas that have witnessed major developments and are written by top experts including:computer simulations of folding, fast folding, single molecule spectroscopy, protein design, aggregation studies (both computational and experimental). Readers will obtain a unique perspective of the problems faced in the biophysical study of protein conformational behaviour in aqueous solution and how these problems are being solved with a multidisciplinary approach that combines theory, experiment and computer simulations. Protein Folding, Misfolding and Aggregation Classical Themes and Novel Approaches is essential reading for graduate students actively involved in protein folding research, other scientists interested in the recent progress of the field and instructors revamping the protein folding section of their biochemistry and biophysics courses.
Investigating the Molecular Basis of Protein Aggregation and Alzheimer's Disease with Multi-scale Computer Simulations
Investigating the Molecular Basis of Protein Aggregation and Alzheimer's Disease with Multi-scale Computer Simulations - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Investigating the Molecular Basis of Protein Aggregation and Alzheimer's Disease with Multi-scale Computer Simulations write by Mary Griffin Krone. This book was released on 2007. Investigating the Molecular Basis of Protein Aggregation and Alzheimer's Disease with Multi-scale Computer Simulations available in PDF, EPUB and Kindle. A number of disorders are associated with the deposition of a variety insoluble protein aggregates. A great deal of evidence has led to the hypothesis that there exists a commonality between the aggregation processes of this diverse body of proteins and peptides. Understanding the universally applicable factors which influence the formation and stability of protein aggregates will provide a better understanding of the diseases to which they lead. To this end, we study several intrinsic and environmental factors which influence protein aggregation, including the role of water, the amount of frustration inherent in the individual proteins, confinement, and protein-surface interactions.
Computer Simulations of Protein Folding and Aggregation
Computer Simulations of Protein Folding and Aggregation - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Computer Simulations of Protein Folding and Aggregation write by . This book was released on 2004. Computer Simulations of Protein Folding and Aggregation available in PDF, EPUB and Kindle. Computer simulation is used to study the competition between protein folding and aggregation, especially the formation of ordered structures that are also known as amyloid fibrils. Employing simplified protein models, we simulate multi-protein systems at a greater level of detail than has previously been possible, probe the fundamental physics that govern protein folding and aggregation, and explore the energetic and structural characteristics of amorphous and fibrillar protein aggregates. We first tackle the aggregation problem by using a low-resolution model called the lattice HP model developed by Lau and Dill. Dynamic Monte Carlo simulations are conducted on a system of simple, two-dimensional lattice protein molecules. We investigate how changing the rate of chemical or thermal renaturation affects the folding and aggregation behavior of the model protein molecule by simulating three renaturation methods: infinitely slow cooling, slow but finite cooling, and quenching. We find that the infinitely slow cooling method provides the highest refolding yields. We then study how the variation of protein concentration affects the refolding yield by simulating the pulse renaturation method, in which denatured proteins are slowly added to the refolding simulation box in a stepwise manner. We observe that the pulse renaturation method provides refolding yields that are substantially higher than those observed in the other three methods even at high packing fractions. We then investigate the folding of a polyalanine peptide with the sequence Ac-KA14K-NH2 using a novel off-lattice, intermediate-resolution protein model originally developed by Smith and Hall. The thermodynamics of a system containing a single Ac-KA14K-NH2 molecule is explored by employing the replica exchange simulation method to map out the conformational transitions as a function of temperature. We also explore the influence of solvent type on the folding process by varying the relative strength of the sid.
Computer Simulations of Protein Structures and Interactions
Computer Simulations of Protein Structures and Interactions - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Computer Simulations of Protein Structures and Interactions write by Serafin Fraga. This book was released on 1995-08-18. Computer Simulations of Protein Structures and Interactions available in PDF, EPUB and Kindle. Protein engineering endeavors to design new peptides and proteins or to change the structural and/or functional characteristics of existing ones for specific purposes, opening the way for the development of new drugs. This work develops in a comprehensive way the theoretical formulation for the methods used in computer-assisted modeling and predictions, starting from the basic concepts and proceeding to the more sophisticated methods, such as Monte Carlo and molecular dynamics. An evaluation of the approximations inherent to the simulations will allow the reader to obtain a perspective of the possible deficiencies and difficulties and approach the task with realistic expectations. Examples from the authors laboratories, as well as from the literature provide useful information.
