Critical Electric Field Quantification for Inducing Bacterial Electroporation - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Critical Electric Field Quantification for Inducing Bacterial Electroporation write by Sijie Chen. This book was released on 2019. Critical Electric Field Quantification for Inducing Bacterial Electroporation available in PDF, EPUB and Kindle. Electroporation is one of the most popular methods for rapid and efficient intracellular transport of foreign molecules, such as peptides, drugs, and DNA, and is crucial for applications in genetic engineering. However, there is still a lack of mechanistic understanding of electroporation, and the most common method to optimize electroporation parameters for bacterial genetic transformation is still based on trial and error. In this work, to alleviate this issue we use microfluidic techniques to achieve rapid screening of electric fields for electroporation in live bacteria. To determine the critical electric field required for inducing bacterial electroporation, we extend upon a previously developed microfluidic assay. By quantifying the critical electric field values of gram-positive bacteria Corynebacterium glutamicum and Bacillus subtilis 168, and the gram-negative bacteria Escherichia coli BL21 (DE3) and Pseudomonas syringae ESC 10 in 10% glycerol and 0.01 x PBS, we study the effects of using different buffer solutions for different bacteria in the electroporation process. We also investigate the relationship between cell polarizability and critical electric field for these cells in the aforementioned buffer solutions. Next, we discuss the accuracy of the measurement by illuminating how undesired phenomena such as electroosmosis and electrophoresis contribute to the measurement error. Results of this study will enhance our understanding of the electroporation process and provide insights in discovering and optimizing electroporation protocols.
Electromanipulation of Cells
Electromanipulation of Cells - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Electromanipulation of Cells write by Ulrich Zimmermann. This book was released on 1996-02-16. Electromanipulation of Cells available in PDF, EPUB and Kindle. Electromanipulation of Cells is the first comprehensive, balanced overview of this dynamic discipline. Edited by leading authorities in the field, the book surveys state-of-the-art research as well as recent practical applications of electric field technologies.
1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies
1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies write by Tomaz Jarm. This book was released on 2015-08-31. 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies available in PDF, EPUB and Kindle. This volume presents the proceedings of the 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies (WC2015). The congress took place in Portorož, Slovenia, during the week of September 6th to 10th, 2015. The scientific part of the Congress covered different aspects of electroporation and related technologies and included the following main topics: · Application of pulsed electric fields technology in food: challenges and opportunities · Electrical impedance measurement for assessment of electroporation yield · Electrochemistry and electroporation · Electroporation meets electrostimulation · Electrotechnologies for food and biomass treatment · Food and biotechnology applications · In vitro electroporation - basic mechanisms · Interfacial behaviour of lipid-assemblies, membranes and cells in electric fields · Irreversible electroporation in clinical use · Medical applications: electrochemotherapy · Medical applications: gene therapy · Non-electric field-based physical methods inducing cell poration and enhanced molecule transfer · Non-thermal plasmas for food safety, environmental applications and medical treatments · PEF for the food industry: fundamentals and applications · PEF proce ss integration - complex process chains and process combinations in the food industry · Predictable animal models · Pulsed electric fields and electroporation technologies in bioeconomy · Veterinary medical applications
Cell Analysis on Microfluidics
Cell Analysis on Microfluidics - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Cell Analysis on Microfluidics write by Jin-Ming Lin. This book was released on 2017-10-25. Cell Analysis on Microfluidics available in PDF, EPUB and Kindle. This book presents a detailed overview of the design, formatting, application, and development of microfluidic chips in the context of cell biology research, enumerating each element involved in microfluidics-based cell analysis, discussing its history, status quo, and future prospects, It also offers an extensive review of the research completed in the past decade, including numerous color figures. The individual chapters are based on the respective authors' studies and experiences, providing tips from the frontline to help researchers overcome bottlenecks in their own work. It highlights a number of cutting-edge techniques, such as 3D cell culture, microfluidic droplet technique, and microfluidic chip-mass spectrometry interfaces, offering a first-hand impression of the latest trends in the field and suggesting new research directions. Serving as both an elementary introduction and advanced guidebook, the book interests and inspires scholars and students who are currently studying microfluidics-based cell analysis methods as well as those who wish to do so.
Electroporation and Electrofusion in Cell Biology
Electroporation and Electrofusion in Cell Biology - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Electroporation and Electrofusion in Cell Biology write by C.A. Jordan. This book was released on 2013-11-11. Electroporation and Electrofusion in Cell Biology available in PDF, EPUB and Kindle. Cells can be funny. Try to grow them with a slightly wrong recipe, and they turn over and die. But hit them with an electric field strong enough to knock over a horse, and they do enough things to justify international meetings, to fill a sizable book, and to lead one to speak of an entirely new technology for cell manipulation. The very improbability of these events not only raises questions about why things happen but also leads to a long list of practical systems in which the application of strong electric fields might enable the merger of cell contents or the introduction of alien but vital material. Inevitably, the basic questions and the practical applications will not keep in step. The questions are intrinsically tough. It is hard enough to analyze the action of the relatively weak fields that rotate or align cells, but it is nearly impossible to predict responses to the cell-shredding bursts of electricity that cause them to fuse or to open up to very large molecular assemblies. Even so, theoretical studies and systematic examination of model systems have produced some creditable results, ideas which should ultimately provide hints of what to try next.
