Cytochrome P450 Protocols - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Cytochrome P450 Protocols write by Ian R. Phillips. This book was released on 2008-02-04. Cytochrome P450 Protocols available in PDF, EPUB and Kindle. For this second edition of their much praised Cytochrome P450, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Additional chapters contain readily reproducible techniques for the transfection of hepatocytes for gene regulation studies, P450 reporter gene assays, in situ hybridization, and analysis of genetic polymorphisms. Although the emphasis is on P450s of mammalian origin, many of the readily reproducible methods described are suitable for P450s from any source.
Cytochrome P450 Protocols
Cytochrome P450 Protocols - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Cytochrome P450 Protocols write by Ian R. Phillips. This book was released on 2013-03-09. Cytochrome P450 Protocols available in PDF, EPUB and Kindle. Cytochromes P450 (CYPs) comprise a large superfamily of proteins that are of central importance in the detoxification or activation of a tremendous number of natural and synthetic hydrophobic xenobiotics, including many therapeutic drugs, chemical carcinogens and environmental pollutants. CYPs are important in mediating interactions between an organism and its chemical environment and in the regulation of physiological processes. Cytochrome P450 Protocols, Third Edition focuses on high-throughput methods for the simultaneous analysis of multiple CYPs, substrates or ligands. Although the emphasis is on CYPs of mammalian origin, it reflects an increasing interest in CYPs of bacterial species. Also included are chapters on cytochrome P450 reductase (the redox partner of CYPs) and the flavin-containing monooxygenases (FMOs), and metabolomic and lipidomic approaches for identification of endogenous substrates of CYPs (‘de-orphanizing’ CYP substrates). Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Cytochrome P450 Protocols, Third Edition provides a wide range of techniques accessible to researchers in fields as diverse as biochemistry, molecular biology, pharmacology, toxicology, environmental biology and genetics.
Methods in Molecular Biology: Cytochrome P450 protocols
Author : John M. Walker
Release : 1984
Genre : Molecular biology -- v.236. Plant functional genomics -- v.237. G protein signaling -- v.238. Biopolymer methods in tissue engineering -- v.239. Cell migration in inflammation and immunity -- v.240. Mammalian artificial chromosomes -- v.241. Cell cycle checkpoint control protocols -- v.242. Atomic force microscopy -- v.243. Chiral separations: Methods and protocols -- v.244. Protein purification protocols -- v.245-6. Gene delivery to mammalian cells -- v.247. Drosophila cytogenetics protocols -- v.248. Antibody engineering -- v.249. Cytokine protocols -- v.251. HPLC of peptides and proteins: Methods and protocols -- v.265. RNA interference, editing, and modification -- v.274. Photosynthesis research protocols -- v.318. Plant cell culture protocols -- v.323. Arabidopsis protocols
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Methods in Molecular Biology: Cytochrome P450 protocols - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Methods in Molecular Biology: Cytochrome P450 protocols write by John M. Walker. This book was released on 1984. Methods in Molecular Biology: Cytochrome P450 protocols available in PDF, EPUB and Kindle.
Cytochrome P450
Cytochrome P450 - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Cytochrome P450 write by Zhengyin Yan. This book was released on 2021. Cytochrome P450 available in PDF, EPUB and Kindle. This collection explores detailed experimental protocols necessary for setting up a variety of in vitro cytochrome P450 (CYP) assays that are vital in selecting drug candidates in a drug discovery pipeline. Major factors affecting drug metabolism include CYP expression levels, kinetic parameters for individual CYP enzymes, CYP inhibition and induction, time-dependent inhibition (TDI), CYP stability, non-CYP stability, UDP-glucuronosyltransferases (UGT) stability, excretion mechanisms, and drug-drug interactions (DDI), all addressed in this volume. Written for the Methods in Pharmacology and Toxicology series, chapters include helpful background information on the in vitro assay, a list of all the materials, reagents, and equipment necessary to carry out the assay, a step-by-step protocol, notes containing common and unexpected experimental problems in the assay, as well as references containing important supplementary reading. Authoritative and practical, Cytochrome P450: In Vitro Methods and Protocols serves as a key guide for researchers in the area of discovery and development of new medicines.
Guide to Cytochromes P450
Guide to Cytochromes P450 - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Guide to Cytochromes P450 write by David F.V. Lewis. This book was released on 1996-07-03. Guide to Cytochromes P450 available in PDF, EPUB and Kindle. This book covers the relevant aspects of cytochromes P450 for understanding their structure, function and mechanisms of action, such as physicochemical characterization methods, redox interactions, evolution and genetics, experimental and theoretical models, induction, inhibition and metabolism.
