Development and Characterization of Microsatellite Markers for the Grain Amaranths (Amaranthus Spp. L.) - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Development and Characterization of Microsatellite Markers for the Grain Amaranths (Amaranthus Spp. L.) write by Melanie Ann Mallory. This book was released on 2007. Development and Characterization of Microsatellite Markers for the Grain Amaranths (Amaranthus Spp. L.) available in PDF, EPUB and Kindle. The grain amaranths (Amaranthus hypochondriacus L., A. cruentus L., and A. caudatus L.) are important pseudocereals native to the Americas that have received increased attention for their nutritional content, specifically their balance of amino acids. The objective of this project was to produce and characterize a set of highly informative, reproducible microsatellite markers for the grain amaranths. A total of 1457 clones were sequenced from three genomic libraries enriched for the microsatellite motifs AAC, AAT and AC. Of these, 353 (24%) contained unique microsatellites. An additional 29 microsatellite loci were identified among 728 BAC-end sequences of a newly developed amaranth BAC library. Flanking primers were designed for 319 of the microsatellite loci and all were screened on a panel of diverse amaranths, including grain and weedy Amaranthus species. A total of 179 (56%) microsatellites were polymorphic across accessions from the three grain amaranths. Among these polymorphic microsatellite loci, a total of 731 alleles were identified with average of four alleles per locus. Heterozygosity values ranged from 0.14 to 0.83 with a mean value of 0.62. Thirty-seven (21%) of the markers were polymorphic between the parents of a segregating population and were shown to be inherited in a normal Mendelian fashion based on chi-squared analysis, demonstrating the utility of these markers for linkage mapping of the amaranth genome. Phylogenetic analysis using the marker data showed A. hybridus accessions in two of the three major grain amaranth clades, suggesting the polyphyletic evolution of the three cultivated species from different A. hybridus ancestors. The microsatellite markers reported here will be useful for further evaluating the relationships among the grain amaranths and their relatives and are an ideal resource for use in marker-assisted breeding programs, germplasm analysis and varietal identification. The transferability of these markers to A. hybridus, A. powellii, and A. retroflexus as reported here suggests that the markers may be useful to other species with the genus Amaranthus, including economically important weeds, vegetable amaranths, and ornamentals.
The Amaranth Genome
The Amaranth Genome - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook The Amaranth Genome write by Dinesh Adhikary. This book was released on 2021-06-01. The Amaranth Genome available in PDF, EPUB and Kindle. This book describes the development of genetic resources in amaranths, with a major focus on genomics, reverse, and forward genetics tools and strategies that have been developed for crop improvement. Amaranth is an ancient crop native to the New World. Interest in amaranths is being renewed, due to their adaptability, stress tolerance, and nutritional value. There are about 65 species in the genus, including Amaranthus caudatus L., A. cruentus L., and A. hypochondriacus L., which are primarily grown as protein-rich grains or pseudocereals. The genus also includes major noxious weeds (e.g., A. palmeri). The amaranths are within the Caryophyllales order and thus many species (e.g., A. tricolor) produce red (betacyanin) or yellow (betaxanthin) betalain pigments, which are chemically distinct from the anthocyanins responsible for red pigmentation in other plants. A. hypochondriacus, which shows disomic inheritance (2n = 32; n= 466 Mb), has been sequenced and annotated with 23,059 protein-coding genes. Additional members of the genus are now also been sequenced including weedy amaranths, other grain amaranths, and their putative progenitors.
Genetics and Genomics of High-Altitude Crops
Genetics and Genomics of High-Altitude Crops - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Genetics and Genomics of High-Altitude Crops write by Vijay Gahlaut. This book was released on . Genetics and Genomics of High-Altitude Crops available in PDF, EPUB and Kindle.
Agricultural Biotechnology: Latest Research and Trends
Agricultural Biotechnology: Latest Research and Trends - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Agricultural Biotechnology: Latest Research and Trends write by Dinesh Kumar Srivastava. This book was released on 2022-01-08. Agricultural Biotechnology: Latest Research and Trends available in PDF, EPUB and Kindle. This book caters to the need of researchers working in the ever-evolving field of agricultural biotechnology. It discusses and provides in-depth information about latest advancements happening in this field. The book discusses evolution of plant tissue culture techniques, development of doubled haploids technology, role of recombinant-DNA technology in crop improvement. It also provides an insight into the global status of genetically modified crops, use of RNAi technology and mi-RNAs in plant improvement. Chapters are also dedicated for different branches of ‘omics’ science including genomics, bioinformatics, proteomics, metabolomics and phenomics along with the use of molecular markers in tagging and mapping of various genes/QTLs of agronomic importance. This book also covers the role of enzymes and microbes in agriculture in productivity enhancement. It is of interest to teachers, researchers of biotechnology and agriculture scientists. Also the book serves as additional reading material for undergraduate and postgraduate students of biotechnology, agriculture, horticulture, forestry, ecology, soil science, and environmental sciences. National and international biotechnologists and agricultural scientists will also find this to be a useful read.
Genomic Designing for Abiotic Stress Resistant Vegetable Crops
Genomic Designing for Abiotic Stress Resistant Vegetable Crops - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Genomic Designing for Abiotic Stress Resistant Vegetable Crops write by Chittaranjan Kole. This book was released on 2022-08-21. Genomic Designing for Abiotic Stress Resistant Vegetable Crops available in PDF, EPUB and Kindle. This book presents deliberations on molecular and genomic mechanisms underlying the interactions of crop plants to the abiotic stresses caused by heat, cold, drought, flooding, submergence, salinity, acidity, etc., important to develop resistant crop varieties. Knowledge on the advanced genetic and genomic crop improvement strategies including molecular breeding, transgenics, genomic-assisted breeding, and the recently emerging genome editing for developing resistant varieties in vegetable crops is imperative for addressing FHNEE (food, health, nutrition, energy, and environment) security. Whole genome sequencing of these crops followed by genotyping-by-sequencing has provided precise information regarding the genes conferring resistance useful for gene discovery, allele mining, and shuttle breeding which in turn opened up the scope for 'designing' crop genomes with resistance to abiotic stresses. The nine chapters each dedicated to a vegetable crop or crop group in this volume elucidate on different types of abiotic stresses and their effects on and interaction with the crop; enumerate on the available genetic diversity with regard to abiotic stress resistance among available cultivars; illuminate on the potential gene pools for utilization in interspecific gene transfer; present brief on classical genetics of stress resistance and traditional breeding for transferring them to their cultivated counterparts; depict the success stories of genetic engineering for developing abiotic stress-resistant crop varieties; discuss on molecular mapping of genes and QTLs underlying stress resistance and their marker-assisted introgression into elite varieties; enunciate on different genomics-aided techniques including genomic selection, allele mining, gene discovery, and gene pyramiding for developing adaptive crop varieties with higher quantity and quality of yields, and also elaborate some case studies on genome editing focusing on specific genes for generating abiotic stress-resistant crops
