Development of a Novel Assay for Molecular Detection and Quantification of Bacteria Directly in Whole Human Blood - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Development of a Novel Assay for Molecular Detection and Quantification of Bacteria Directly in Whole Human Blood write by April Joy Aralar. This book was released on 2023. Development of a Novel Assay for Molecular Detection and Quantification of Bacteria Directly in Whole Human Blood available in PDF, EPUB and Kindle. Bacteria are all around us. In our environment, on our bodies, and even in the air we breathe. While usually benign, their invasion into the bloodstream can pose severe risks to human health making detection of these microbes of the utmost importance. Typical bacterial detection techniques for clinical diagnostics are limited by long testing times, high rates of false positive and negative detection, and assumptions about the pathogen. This dissertation is centered around the development of a novel method based on digital PCR (dPCR) to accurately and agnostically detect, identify, and quantify bacteria for applications in clinical microbiology. In chapter 1, I developed a novel high-resolution melt (HRM)-based internal amplification control (IAC) strategy for dPCR to provide assurance that clinical specimens are successfully amplified and accurately quantified. I showed that detection of an exogenous IAC using digital HRM (dHRM) reduces the inclusion of false negative partitions, changing the calculated DNA concentration up to 52%. The integration of dHRM enabled classification of partitions that would otherwise be considered ambiguous "rain", which accounts for up to ~3% and ~10% of partitions in intercalating dye and hydrolysis probe dPCR respectively. The IAC was developed for use with a broad-based bacterial primer set for microbial profiling, but can be applied to any dPCR application. I then took these concepts and used them to develop a novel method to detect, quantify, and identify bacteria in human blood samples. This first involved optimizing the assay developed in chapter 1 to avoid interference from human DNA. I then developed a melt curve database for bacterial pathogens using dHRM, quantified the lower limit of detection for our method, and used the assay to quantify bacterial DNA in whole blood samples that were matched to blood culture positives. This study demonstrated that dHRM can be reliably used to differentiate blood culture positive and negative samples from patients with 90% concordance when compared with clinical adjudication. This work resulted in a technique that can be applied to a number of DNA profiling applications and advances the utility of dPCR in clinical applications where accurate quantification is imperative.
Molecular Biology of The Cell
Molecular Biology of The Cell - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Molecular Biology of The Cell write by Bruce Alberts. This book was released on 2002. Molecular Biology of The Cell available in PDF, EPUB and Kindle.
The Prokaryotes
The Prokaryotes - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook The Prokaryotes write by Edward F. DeLong. This book was released on 2014-10-13. The Prokaryotes available in PDF, EPUB and Kindle. The Prokaryotes is a comprehensive, multi-authored, peer reviewed reference work on Bacteria and Achaea. This fourth edition of The Prokaryotes is organized to cover all taxonomic diversity, using the family level to delineate chapters. Different from other resources, this new Springer product includes not only taxonomy, but also prokaryotic biology and technology of taxa in a broad context. Technological aspects highlight the usefulness of prokaryotes in processes and products, including biocontrol agents and as genetics tools. The content of the expanded fourth edition is divided into two parts: Part 1 contains review chapters dealing with the most important general concepts in molecular, applied and general prokaryote biology; Part 2 describes the known properties of specific taxonomic groups. Two completely new sections have been added to Part 1: bacterial communities and human bacteriology. The bacterial communities section reflects the growing realization that studies on pure cultures of bacteria have led to an incomplete picture of the microbial world for two fundamental reasons: the vast majority of bacteria in soil, water and associated with biological tissues are currently not culturable, and that an understanding of microbial ecology requires knowledge on how different bacterial species interact with each other in their natural environment. The new section on human microbiology deals with bacteria associated with healthy humans and bacterial pathogenesis. Each of the major human diseases caused by bacteria is reviewed, from identifying the pathogens by classical clinical and non-culturing techniques to the biochemical mechanisms of the disease process. The 4th edition of The Prokaryotes is the most complete resource on the biology of prokaryotes. The following volumes are published consecutively within the 4th Edition: Prokaryotic Biology and Symbiotic Associations Prokaryotic Communities and Ecophysiology Prokaryotic Physiology and Biochemistry Applied Bacteriology and Biotechnology Human Microbiology Actinobacteria Firmicutes Alphaproteobacteria and Betaproteobacteria Gammaproteobacteria Deltaproteobacteria and Epsilonproteobacteria Other Major Lineages of Bacteria and the Archaea
Global Infectious Disease Surveillance and Detection
Global Infectious Disease Surveillance and Detection - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Global Infectious Disease Surveillance and Detection write by Institute of Medicine. This book was released on 2007-11-11. Global Infectious Disease Surveillance and Detection available in PDF, EPUB and Kindle. Early detection is essential to the control of emerging, reemerging, and novel infectious diseases, whether naturally occurring or intentionally introduced. Containing the spread of such diseases in a profoundly interconnected world requires active vigilance for signs of an outbreak, rapid recognition of its presence, and diagnosis of its microbial cause, in addition to strategies and resources for an appropriate and efficient response. Although these actions are often viewed in terms of human public health, they also challenge the plant and animal health communities. Surveillance, defined as "the continual scrutiny of all aspects of occurrence and spread of a disease that are pertinent to effective control", involves the "systematic collection, analysis, interpretation, and dissemination of health data." Disease detection and diagnosis is the act of discovering a novel, emerging, or reemerging disease or disease event and identifying its cause. Diagnosis is "the cornerstone of effective disease control and prevention efforts, including surveillance." Disease surveillance and detection relies heavily on the astute individual: the clinician, veterinarian, plant pathologist, farmer, livestock manager, or agricultural extension agent who notices something unusual, atypical, or suspicious and brings this discovery in a timely way to the attention of an appropriate representative of human public health, veterinary medicine, or agriculture. Most developed countries have the ability to detect and diagnose human, animal, and plant diseases. Global Infectious Disease Surveillance and Detection: Assessing the Challenges-Finding Solutions, Workshop Summary is part of a 10 book series and summarizes the recommendations and presentations of the workshop.
Molecular Detection of Human Bacterial Pathogens
Molecular Detection of Human Bacterial Pathogens - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Molecular Detection of Human Bacterial Pathogens write by Dongyou Liu. This book was released on 2011-04-18. Molecular Detection of Human Bacterial Pathogens available in PDF, EPUB and Kindle. As more original molecular protocols and subsequent modifications are described in the literature, it has become difficult for those not directly involved in the development of these protocols to know which are most appropriate to adopt for accurate identification of bacterial pathogens. Molecular Detection of Human Bacterial Pathogens addresses th
