Electroporation Protocols for Microorganisms - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Electroporation Protocols for Microorganisms write by Jac A. Nickoloff. This book was released on 2008-02-02. Electroporation Protocols for Microorganisms available in PDF, EPUB and Kindle. Electroporation is one of the most widespread techniques used in modem molecular genetics. It is most commonly used to introduce DNA into cells for investigations of gene structure and function, and in this regard, electroporation is both highly versatile, being effective with nearly all species and cell types, and highly efficient. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. However, exposure of cells to brief, hi- intensity electric fields has found broad application in other aspects of biological research, and is now routinely used to introduce other types of biological and analytic molecules into cells, to induce cell-cell fusion, and to transfer DNA directly between different species. The first seven chapters of Electroporation Protocols for Micro organisms describe the underlying theory of electroporation, the com mercially available instrumentation, and a number of specialized electroporation applications, such as cDNA library construction and interspecies DNA electrotransfer. Each of the remaining chapters pre sents a well developed method for electrotransformation of a particular bacterial, fungal, or protist species. These chapters also serve to intro duce those new to the field the important research questions that are currently being addressed with particular organisms, highlighting both the major advantages and limitations of each species as a model organ ism, and explaining the roles that electroporation has played in the development of the molecular genetic systems currently in use.
Electrotransformation of Bacteria
Electrotransformation of Bacteria - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Electrotransformation of Bacteria write by Natalie Eynard. This book was released on 2013-06-29. Electrotransformation of Bacteria available in PDF, EPUB and Kindle. In this manual, protocols for the transformation of about 40 strains of bacteria are described, with the emphasis placed on the individual critical procedural steps, since the practical details mainly depend on the bacterial strain under investigation. This presentation together with the theoretical introductionary chapters, allows users to modify and adapt each protocol to their own experiments. Bacterial strains with relevance in the food industry, biotechnology, medical and veterinary fields, agroindustry and environmental sciences are covered.
Methods in Molecular Biology: Electroporation protocols for microorganisms
Author : John M. Walker
Release : 1984
Genre : Molecular biology -- v.236. Plant functional genomics -- v.237. G protein signaling -- v.238. Biopolymer methods in tissue engineering -- v.239. Cell migration in inflammation and immunity -- v.240. Mammalian artificial chromosomes -- v.241. Cell cycle checkpoint control protocols -- v.242. Atomic force microscopy -- v.243. Chiral separations: Methods and protocols -- v.244. Protein purification protocols -- v.245-6. Gene delivery to mammalian cells -- v.247. Drosophila cytogenetics protocols -- v.248. Antibody engineering -- v.249. Cytokine protocols -- v.251. HPLC of peptides and proteins: Methods and protocols -- v.265. RNA interference, editing, and modification -- v.274. Photosynthesis research protocols -- v.318. Plant cell culture protocols -- v.323. Arabidopsis protocols
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Methods in Molecular Biology: Electroporation protocols for microorganisms - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Methods in Molecular Biology: Electroporation protocols for microorganisms write by John M. Walker. This book was released on 1984. Methods in Molecular Biology: Electroporation protocols for microorganisms available in PDF, EPUB and Kindle.
Guide to Electroporation and Electrofusion
Guide to Electroporation and Electrofusion - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Guide to Electroporation and Electrofusion write by Donald C. Chang. This book was released on 2012-12-02. Guide to Electroporation and Electrofusion available in PDF, EPUB and Kindle. Electroporation is an efficient method to introduce macromolecules such as DNA into a wide variety of cells. Electrofusion results in the fusion of cells and can be used to produce genetic hybrids or hybridoma cells. Guide to Electroporation and Electrofusion is designed to serve the needs of students, experienced researchers, and newcomers to the field. It is a comprehensive manual that presents, in one source, up-to-date, easy-to-follow protocols necessary for efficient electroporation and electrofusion of bacteria, yeast, and plant and animal cells, as well as background information to help users optimize their results through comprehension of the principles behind these techniques. Covers fundamentals of electroporation and electrofusion in detail: Molecular events, Mechanisms, Kinetics, Gives extensive practical information, The latest applications, Controlling parameters to maximize efficiency, Available instrumentation Presents applications of electroporation and electrofusion in current research situations State-of-the-art modifications to electrical pulses and generators Application of electroporation and electrofusion to unique, alternative cell and tissue types Gives straightforward, detailed, easy-to-follow protocols for Formation of human hybridomas Introduction of genetic material into plant cells and pollen Transfection of mammalian cells Transformation of bacteria, plants, and yeast Production of altered embryos Optimization of electroporation by using reporter genes Comprehensive and up-to-date Convenient bench-top format Approximately 125 illustrations complement the text Complete references with article titles Written by leading authorities in electroporation and electrofusion
Critical Electric Field Quantification for Inducing Bacterial Electroporation
Critical Electric Field Quantification for Inducing Bacterial Electroporation - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Critical Electric Field Quantification for Inducing Bacterial Electroporation write by Sijie Chen. This book was released on 2019. Critical Electric Field Quantification for Inducing Bacterial Electroporation available in PDF, EPUB and Kindle. Electroporation is one of the most popular methods for rapid and efficient intracellular transport of foreign molecules, such as peptides, drugs, and DNA, and is crucial for applications in genetic engineering. However, there is still a lack of mechanistic understanding of electroporation, and the most common method to optimize electroporation parameters for bacterial genetic transformation is still based on trial and error. In this work, to alleviate this issue we use microfluidic techniques to achieve rapid screening of electric fields for electroporation in live bacteria. To determine the critical electric field required for inducing bacterial electroporation, we extend upon a previously developed microfluidic assay. By quantifying the critical electric field values of gram-positive bacteria Corynebacterium glutamicum and Bacillus subtilis 168, and the gram-negative bacteria Escherichia coli BL21 (DE3) and Pseudomonas syringae ESC 10 in 10% glycerol and 0.01 x PBS, we study the effects of using different buffer solutions for different bacteria in the electroporation process. We also investigate the relationship between cell polarizability and critical electric field for these cells in the aforementioned buffer solutions. Next, we discuss the accuracy of the measurement by illuminating how undesired phenomena such as electroosmosis and electrophoresis contribute to the measurement error. Results of this study will enhance our understanding of the electroporation process and provide insights in discovering and optimizing electroporation protocols.
