Electrotransformation of Bacteria - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Electrotransformation of Bacteria write by Natalie Eynard. This book was released on 2013-06-29. Electrotransformation of Bacteria available in PDF, EPUB and Kindle. In this manual, protocols for the transformation of about 40 strains of bacteria are described, with the emphasis placed on the individual critical procedural steps, since the practical details mainly depend on the bacterial strain under investigation. This presentation together with the theoretical introductionary chapters, allows users to modify and adapt each protocol to their own experiments. Bacterial strains with relevance in the food industry, biotechnology, medical and veterinary fields, agroindustry and environmental sciences are covered.
Handbook of Electroporation
Handbook of Electroporation - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Handbook of Electroporation write by Damijan Miklavčič. This book was released on 2017-09-14. Handbook of Electroporation available in PDF, EPUB and Kindle. This major reference work is a one-shot knowledge base on electroporation and the use of pulsed electric fields of high intensity and their use in biology, medicine, biotechnology, and food and environmental technologies. The Handbook offers a widespread and well-structured compilation of 156 chapters ranging from the foundations to applications in industry and hospital. It is edited and written by most prominent researchers in the field. With regular updates and growing in its volume it is suitable for academic readers and researchers regardless of their disciplinary expertise, and will also be accessible to students and serious general readers. The Handbook's 276 authors have established scholarly credentials and come from a wide range of disciplines. This is crucially important in a highly interdisciplinary field of electroporation and the use of pulsed electric fields of high intensity and its applications in different fields from medicine, biology, food processing, agriculture, process engineering, energy and environment. An Editorial Board of distinguished scholars from across the world has selected and reviewed the various chapters to ensure the highest quality of this Handbook. The book was edited by an international team of Section Editors: P. Thomas Vernier, Boris Rubinsky, Juergen Kolb, Damijan Miklavcic, Marie-Pierre Rols, Javier Raso, Richard Heller, Gregor Serša, Dietrich Knorr, and Eugene Vorobiev.
Electroporation Protocols for Microorganisms
Electroporation Protocols for Microorganisms - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Electroporation Protocols for Microorganisms write by Jac A. Nickoloff. This book was released on 2008-02-02. Electroporation Protocols for Microorganisms available in PDF, EPUB and Kindle. Electroporation is one of the most widespread techniques used in modem molecular genetics. It is most commonly used to introduce DNA into cells for investigations of gene structure and function, and in this regard, electroporation is both highly versatile, being effective with nearly all species and cell types, and highly efficient. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. However, exposure of cells to brief, hi- intensity electric fields has found broad application in other aspects of biological research, and is now routinely used to introduce other types of biological and analytic molecules into cells, to induce cell-cell fusion, and to transfer DNA directly between different species. The first seven chapters of Electroporation Protocols for Micro organisms describe the underlying theory of electroporation, the com mercially available instrumentation, and a number of specialized electroporation applications, such as cDNA library construction and interspecies DNA electrotransfer. Each of the remaining chapters pre sents a well developed method for electrotransformation of a particular bacterial, fungal, or protist species. These chapters also serve to intro duce those new to the field the important research questions that are currently being addressed with particular organisms, highlighting both the major advantages and limitations of each species as a model organ ism, and explaining the roles that electroporation has played in the development of the molecular genetic systems currently in use.
Streptococcal Genetics
Streptococcal Genetics - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Streptococcal Genetics write by Joseph J. Ferretti. This book was released on 1987. Streptococcal Genetics available in PDF, EPUB and Kindle.
Electroporation and Electrofusion in Cell Biology
Electroporation and Electrofusion in Cell Biology - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Electroporation and Electrofusion in Cell Biology write by C.A. Jordan. This book was released on 2013-11-11. Electroporation and Electrofusion in Cell Biology available in PDF, EPUB and Kindle. Cells can be funny. Try to grow them with a slightly wrong recipe, and they turn over and die. But hit them with an electric field strong enough to knock over a horse, and they do enough things to justify international meetings, to fill a sizable book, and to lead one to speak of an entirely new technology for cell manipulation. The very improbability of these events not only raises questions about why things happen but also leads to a long list of practical systems in which the application of strong electric fields might enable the merger of cell contents or the introduction of alien but vital material. Inevitably, the basic questions and the practical applications will not keep in step. The questions are intrinsically tough. It is hard enough to analyze the action of the relatively weak fields that rotate or align cells, but it is nearly impossible to predict responses to the cell-shredding bursts of electricity that cause them to fuse or to open up to very large molecular assemblies. Even so, theoretical studies and systematic examination of model systems have produced some creditable results, ideas which should ultimately provide hints of what to try next.
