Fluorescence Spectroscopy and Microscopy - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Fluorescence Spectroscopy and Microscopy write by Yves Engelborghs. This book was released on 2014. Fluorescence Spectroscopy and Microscopy available in PDF, EPUB and Kindle. Reflecting the expanding field's need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results.
Handbook of Fluorescence Spectroscopy and Imaging
Handbook of Fluorescence Spectroscopy and Imaging - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Handbook of Fluorescence Spectroscopy and Imaging write by Markus Sauer. This book was released on 2010-12-23. Handbook of Fluorescence Spectroscopy and Imaging available in PDF, EPUB and Kindle. Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.
Principles of Fluorescence Spectroscopy
Principles of Fluorescence Spectroscopy - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Principles of Fluorescence Spectroscopy write by Joseph R. Lakowicz. This book was released on 2013-11-11. Principles of Fluorescence Spectroscopy available in PDF, EPUB and Kindle. Fluorescence methods are being used increasingly in biochemical, medical, and chemical research. This is because of the inherent sensitivity of this technique. and the favorable time scale of the phenomenon of fluorescence. 8 Fluorescence emission occurs about 10- sec (10 nsec) after light absorp tion. During this period of time a wide range of molecular processes can occur, and these can effect the spectral characteristics of the fluorescent compound. This combination of sensitivity and a favorable time scale allows fluorescence methods to be generally useful for studies of proteins and membranes and their interactions with other macromolecules. This book describes the fundamental aspects of fluorescence. and the biochemical applications of this methodology. Each chapter starts with the -theoreticalbasis of each phenomenon of fluorescence, followed by examples which illustrate the use of the phenomenon in the study of biochemical problems. The book contains numerous figures. It is felt that such graphical presentations contribute to pleasurable reading and increased understand ing. Separate chapters are devoted to fluorescence polarization, lifetimes, quenching, energy transfer, solvent effects, and excited state reactions. To enhance the usefulness of this work as a textbook, problems are included which illustrate the concepts described in each chapter. Furthermore, a separate chapter is devoted to the instrumentation used in fluorescence spectroscopy. This chapter will be especially valuable for those perform ing or contemplating fluorescence measurements. Such measurements are easily compromised by failure to consider a number of simple principles.
New Trends in Fluorescence Spectroscopy
New Trends in Fluorescence Spectroscopy - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook New Trends in Fluorescence Spectroscopy write by Bernard Valeur. This book was released on 2012-12-06. New Trends in Fluorescence Spectroscopy available in PDF, EPUB and Kindle. This first volume in the new Springer Series on Fluorescence brings together fundamental and applied research from this highly interdisciplinary and field, ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, sensor applications, confocal microscopy and protein-protein interactions. This carefully edited collection of articles is an invaluable tool for practitioners and novices.
Fluorescence Spectroscopy and Microscopy in Biology
Fluorescence Spectroscopy and Microscopy in Biology - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Fluorescence Spectroscopy and Microscopy in Biology write by Radek Šachl. This book was released on 2023-04-27. Fluorescence Spectroscopy and Microscopy in Biology available in PDF, EPUB and Kindle. This book provides the reader with an updated comprehensive view of the rapidly developing and fascinating field of fluorescence spectroscopy and microscopy. In recent years, fluorescence spectroscopy and microscopy have experienced rapid technological development, which has enabled the detection and monitoring of single molecules with high spatial and temporal resolution. Thanks to these developments, fluorescence has become an even more popular method in physical, biological and related fields. This book guides the reader through both basic and advanced fluorescence spectroscopy and microscopy approaches with a focus on their applications in membrane and protein biophysics. Each of the four parts: A - Fluorescence Spectroscopy, B - Fluorescence Microscopy, C - Applications of Fluorescence Spectroscopy and Microscopy to biological membranes and D - Applications of Fluorescence Spectroscopy to protein studies are written by experts within the field. The book is intended for both complete beginners who want to quickly orient themselves in the large number of existing fluorescent methods, as well as for advanced readers who are interested in particular methods and their proper use.
