Homing Endonucleases and Inteins - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Homing Endonucleases and Inteins write by Marlene Belfort. This book was released on 2008-09-22. Homing Endonucleases and Inteins available in PDF, EPUB and Kindle. This book provides the first and only comprehensive description and detailed summary of the genetics, structure, function, mechanisms of action, evolution and engineering of homing endonucleases and inteins. These two unique protein superfamilies, which are tied together through their frequent fusion and coevolution, have generated considerable excitement for their fundamental, structural, and functional properties, their evolution as parasitic elements, and their widespread applications as gene targeting agents and as instruments for the generation of modified proteins and novel protein combinations.
Studies on the Evolution, Structure, and Function of Homing Endonuclease Containing Parasitic Genetic Elements
Studies on the Evolution, Structure, and Function of Homing Endonuclease Containing Parasitic Genetic Elements - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Studies on the Evolution, Structure, and Function of Homing Endonuclease Containing Parasitic Genetic Elements write by Alireza Ghodsi Senejani. This book was released on 2007. Studies on the Evolution, Structure, and Function of Homing Endonuclease Containing Parasitic Genetic Elements available in PDF, EPUB and Kindle.
Evolutionary Relationships, Design, and Biochemical Characterization of Homing Endonucleases
Evolutionary Relationships, Design, and Biochemical Characterization of Homing Endonucleases - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Evolutionary Relationships, Design, and Biochemical Characterization of Homing Endonucleases write by Gregory K. Taylor. This book was released on 2011. Evolutionary Relationships, Design, and Biochemical Characterization of Homing Endonucleases available in PDF, EPUB and Kindle. Homing endonucleases, found in all forms of microbial life, facilitate the invasion of host genes often in concert with introns or inteins by generating double stranded breaks in conserved coding sequences. There are five homing endonuclease families distinct in their structural characteristics and each family appears to share a common ancestor with diverse host proteins of unrelated function. Such related proteins include restriction endonucleases, DNA mismatch repair proteins, transcription factors, four-way junction resolving enzymes, and colicins. Homing endonculeases are currently being computationally redesigned for applications in genome engineering and structures of three redesigned homing endonuclease variants are described. In these experiments, crystal structures uncovered unexpected shifts in the DNA backbone relative to the wild type endonucleases and have thus been informative in the redesign process. Recently, a sixth homing endonuclease family homologous to E. Coli DNA repair protein VSR was discovered. A series of biochemical and x-ray crystallographic experiments investigating binding specificity and catalytic mechanism of a representative family member I-Bth0305I are described. Finally, a database archiving experimentally characterized homing endonucleases and a web-base program supporting homing endonuclease target site search are discussed.
Split Inteins
Split Inteins - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Split Inteins write by Henning D. Mootz. This book was released on 2016-10-07. Split Inteins available in PDF, EPUB and Kindle. This volume focuses on applications of split inteins, and the progress that has been made in the past 5 years on discovery and engineering of fast and more efficient split inteins. The first few chapters in Split Inteins: Methods and Protocols explore new techniques on how to use split inteins for affinity purification of overproduced proteins, and split-intein based technologies to prepare cyclic peptides and proteins. The next few chapters discuss semisynthetic protein trans-splicing using one synthetic intein piece, synthetic intein-extein pieces used to deliver other cargos for chemical modification both of purified proteins and of proteins in living cells, as well as isotopic labeling of proteins for NMR studies, and a discussion on how protein block copolymers can be generated by protein trans-splicing to form protein hydrogels. The last few chapters deal with intein applications in transgenic plants and conditional inteins that can be regulated in artificial ways by small molecules or light, a cassette-based approach to quickly test many intein insertion positions, and a computational approach to predict new intein split sites (the approach also works for other proteins). Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Split Inteins: Methods and Protocols is a valuable resource that will provide guidance toward possibilities of split intein applications, explore proven and detailed protocols adaptable to various research projects, and inspire new method developments.
Analysis of Fungal Inteins
Analysis of Fungal Inteins - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Analysis of Fungal Inteins write by Annika Anna Maria Bokor. This book was released on 2010. Analysis of Fungal Inteins available in PDF, EPUB and Kindle.
