In Situ Hybridization in Neurobiology - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook In Situ Hybridization in Neurobiology write by James H. Eberwine. This book was released on 1994. In Situ Hybridization in Neurobiology available in PDF, EPUB and Kindle. In situ hybridization has become an important and widely used research tool for neurobiologists since the authors' first book on the subject, In Situ Hybridization: Applications to Neurobiology (Oxford, 1987). This new volume examines the methodological advances that have been made in in situhybridization techniques and their impact on the study of the nervous system. These include methods for improving sensitivity, ways of examining multiple mRNAs within the same section, ways of examining both proteins and mRNAs within the same section, and methods for using in situ hybridizationtechnology to examine areas of cellular biological significance, such as translational control. The development of alternative labels to radioactivity for the detection of hybridized probes, and the combined use of in situ hybridization and immunocytochemistry is also covered in this book. Itdiscusses the quantification of in situ hybridization signal and presents an overview of information generated by using in situ hybridization in diagnostic medicine. This volume will be essential to researchers in the neurosciences as well as those in other disciplines.
In Situ Hybridization Protocols for the Brain
In Situ Hybridization Protocols for the Brain - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook In Situ Hybridization Protocols for the Brain write by W. Wisden. This book was released on 2002-08-05. In Situ Hybridization Protocols for the Brain available in PDF, EPUB and Kindle. This volume of the International Review of Neurobiology was written to assist researchers without any previous experience with in situ hybridization, allowing them to follow the protocols and expect good results. It contains all the information required for newcomers to achieve successful in situ hybridization results, and methods for improving the technique of those already utilizing it. Published since 1959, International Review of Neurobiology is a well-known series appealing to neuroscientists, clinicians, psychologists, physiologists, and pharmacologists. Led by an internationally renowned editorial board, this important serial publishes both eclectic volumes made up of timely reviews and thematic volumes that focus on recent progress in a specific area of neurobiology research. A well-known series appealing to neuroscientists, clinicians, psychologists, physiologists, and pharmacologists Led by an internationally renowned editorial board, this important serial publishes both eclectic volumes made up of timely reviews and thematic volumes that focus on recent progress in a specific area of neurobiology research
In Situ Hybridization in Brain
In Situ Hybridization in Brain - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook In Situ Hybridization in Brain write by George Uhl. This book was released on 2012-12-06. In Situ Hybridization in Brain available in PDF, EPUB and Kindle. The explosion of interest in specific molecules important for brain function and dysfunction has drawn individuals from diverse backgrounds toward the use of in situ hybridization techniques. Study of the brain demands the anatomic precision and biochemical specificity that this approach can potentially bring. Workers with backgrounds in peptide neuroanatomy, neuropharmacology, molecular biology, neurovirology, neuropathology, and neurophysiology have joined together in this volume to discuss their initial experiences in applying ill situ hybridization techniques to the study of the brain. The work, although still in an early phase of development, is worthy of initial summary and dissemination. In the area of neuropeptide gene expression alone, investigators represented here describe studies of vasopressin, opiate peptides, oxytocin, vasoactive intestinal peptide, cholecystokinin, and somatostatin. Other contributions provide insight into applications of the technique to studies of the expression of genes for neurotransmitter synthesizing enzymes, viral-encoded genes, trophic factor genes, and the genes selected on the basis of their special roles in the brain. The authors provide an important series of technical perspectives, and describe specific experimental protocols. This volume should be of interest to individuals seeking an introduction to these methods, as well to those desiring an up to date precis of work in this burgeoning area. Dr. Uhl, with the sponsorship of the Howard Hughes Medical Institute, has done a superb job of assembling the leaders in this area, and in organizing the presen ta tion of their perspecti ves herein. Joseph B. Martin, M.D., Ph.D.
In Situ Hybridization
In Situ Hybridization - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook In Situ Hybridization write by Karen L. Valentino. This book was released on 1987. In Situ Hybridization available in PDF, EPUB and Kindle. A technique for reading the messenger RNAs by which cells express their distinctive populations of structural and functional proteins, in situ hybridization is now reliable and practical for use in neurobiological research.
In Situ Hybridization Techniques for the Brain
In Situ Hybridization Techniques for the Brain - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook In Situ Hybridization Techniques for the Brain write by Z. Henderson. This book was released on 1996-12-11. In Situ Hybridization Techniques for the Brain available in PDF, EPUB and Kindle. Written by molecular biologists and neurobiologists, this volume in the Handbook Series provides basic step-by-step protocols in addition to specialist techniques to support those practising in situ hybridization, a powerful histochemical technique which is now widely used in neurobiology. The first part of the manual concentrates on basic procedures, eg, which probe and label to select, equipment, relevant molecular biological techniques, hazards, probe preparation and labelling, tissue preparation, and conditions for hybridization. Specialist procedures including quantitative analysis of data, reverse transcription PCR, working with human tissue, and double label methods are presented in subsequent sections. In Situ Hybridization Techniques for the Brain is, therefore, an essential reference source for all those with research interests in neuroanatomy, histopathology and/or neurohistochemistry, and will support users in both the preparation and successful completion of the procedures in their own laboratory.
