In Situ Hybridization Protocols - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook In Situ Hybridization Protocols write by Boye Nielsen. This book was released on 2014-09-13. In Situ Hybridization Protocols available in PDF, EPUB and Kindle. In Situ Hybridization Protocols, Fourth Edition contains 21 protocols that utilize the in situ hybridization technology to document or take advantage of the visualization of specific RNA molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, In Situ Hybridization Protocols, Fourth Edition seeks to aid scientists in the further discovery of new RNA species and uncovering of their cellular functions.
In Situ Hybridization Protocols
In Situ Hybridization Protocols - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook In Situ Hybridization Protocols write by Ian A. Darby. This book was released on 2000. In Situ Hybridization Protocols available in PDF, EPUB and Kindle. Annotation Darby (human biology, RMIT U., Victoria, Australia) is joined by geneticists, molecular biologists, and pathologists from around the world to describe basic and advanced techniques for hybridization, for whole-mount embryo specimens and at the electron microscope level. Coverage includes protocols for detection of DNA fragmentation in apoptosis, localization of genes to particular chromosomes, and the use of DNA and RNA probes to detect expression in cells or tissue sections. For novice and experienced investigators who need proven and readily reproducible methods. Annotation c. Book News, Inc., Portland, OR (booknews.com)
In Situ Hybridization Protocols
In Situ Hybridization Protocols - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook In Situ Hybridization Protocols write by Ian A. Darby. This book was released on 2008-02-04. In Situ Hybridization Protocols available in PDF, EPUB and Kindle. The technique of in situ hybridization, in its various forms, has been used routinely in many laboratories for a number of years. In the post-genome era, gene arrays and proteomics have allowed us to identify hitherto unknown unrecognized pathways and mechanisms. However, rather than diminish the importance of in situ hybridization, the now widespread use of screening te- nologies has increased the need to temporally and spatially localize the dist- bution of mRNA expression. Our intention, in In Situ Hybridization Protocols is to provide ample inf- mation for novices planning to set up the in situ hybridization technique and use it in their laboratory for the first time, as well as giving updates of recent developments for those laboratories where in situ hybridization techniques are already in use. Despite its widespread significance, in situ hybridization has retained a re- tation as one of the more difficult and capricious molecular biological te- niques. This may in part be because of the hybrid nature of the technique, which often requires a mixture of molecular biological and histological skills. The two techniques are usually taught and acquired in different streams of biolo- cal science. The step-by-step and detailed protocols provided in In Situ Hybridization Protocols by researchers active in the field should make it p- sible for both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use the technique s- cessfully in their laboratories.
Fluorescence In Situ Hybridization (FISH) - Application Guide
Fluorescence In Situ Hybridization (FISH) - Application Guide - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Fluorescence In Situ Hybridization (FISH) - Application Guide write by Thomas Liehr. This book was released on 2008-11-26. Fluorescence In Situ Hybridization (FISH) - Application Guide available in PDF, EPUB and Kindle. This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of them are presented in 36 chapters by authors from ten different countries around the world. The book not only provides the reader with basic and background knowledge on the topic, but also gives detailed protocols that show how molecular cytogenetics is currently performed by specialists in this field. The FISH Application Guide initially provides an overview of the (historical) development of molecular cytogenetics, its basic procedures, the equipment required, and probe generation. The book then describes tips and tricks for making different tissues available for molecular cytogenetic studies. These are followed by chapters on various multicolor FISH probe sets, their availability, and their pot- tial for use in combination with other approaches. The possible applications that are shown encompass the characterization of marker chromosomes, cryptic cytogenetic aberrations and epigenetic changes in humans by interphase and metaphase cyto- netics, studies of nuclear architecture, as well as the application of molecular cytogenetics to zoology, botany and microbiology.
In Situ Hybridization Protocols for the Brain
In Situ Hybridization Protocols for the Brain - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook In Situ Hybridization Protocols for the Brain write by W. Wisden. This book was released on 2002-08-05. In Situ Hybridization Protocols for the Brain available in PDF, EPUB and Kindle. This volume of the International Review of Neurobiology was written to assist researchers without any previous experience with in situ hybridization, allowing them to follow the protocols and expect good results. It contains all the information required for newcomers to achieve successful in situ hybridization results, and methods for improving the technique of those already utilizing it. Published since 1959, International Review of Neurobiology is a well-known series appealing to neuroscientists, clinicians, psychologists, physiologists, and pharmacologists. Led by an internationally renowned editorial board, this important serial publishes both eclectic volumes made up of timely reviews and thematic volumes that focus on recent progress in a specific area of neurobiology research. A well-known series appealing to neuroscientists, clinicians, psychologists, physiologists, and pharmacologists Led by an internationally renowned editorial board, this important serial publishes both eclectic volumes made up of timely reviews and thematic volumes that focus on recent progress in a specific area of neurobiology research
