In Situ Hybridization Protocols for the Brain - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook In Situ Hybridization Protocols for the Brain write by W. Wisden. This book was released on 2002-08-05. In Situ Hybridization Protocols for the Brain available in PDF, EPUB and Kindle. This volume of the International Review of Neurobiology was written to assist researchers without any previous experience with in situ hybridization, allowing them to follow the protocols and expect good results. It contains all the information required for newcomers to achieve successful in situ hybridization results, and methods for improving the technique of those already utilizing it. Published since 1959, International Review of Neurobiology is a well-known series appealing to neuroscientists, clinicians, psychologists, physiologists, and pharmacologists. Led by an internationally renowned editorial board, this important serial publishes both eclectic volumes made up of timely reviews and thematic volumes that focus on recent progress in a specific area of neurobiology research. A well-known series appealing to neuroscientists, clinicians, psychologists, physiologists, and pharmacologists Led by an internationally renowned editorial board, this important serial publishes both eclectic volumes made up of timely reviews and thematic volumes that focus on recent progress in a specific area of neurobiology research
In Situ Hybridization in Brain
In Situ Hybridization in Brain - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook In Situ Hybridization in Brain write by George Uhl. This book was released on 2012-12-06. In Situ Hybridization in Brain available in PDF, EPUB and Kindle. The explosion of interest in specific molecules important for brain function and dysfunction has drawn individuals from diverse backgrounds toward the use of in situ hybridization techniques. Study of the brain demands the anatomic precision and biochemical specificity that this approach can potentially bring. Workers with backgrounds in peptide neuroanatomy, neuropharmacology, molecular biology, neurovirology, neuropathology, and neurophysiology have joined together in this volume to discuss their initial experiences in applying ill situ hybridization techniques to the study of the brain. The work, although still in an early phase of development, is worthy of initial summary and dissemination. In the area of neuropeptide gene expression alone, investigators represented here describe studies of vasopressin, opiate peptides, oxytocin, vasoactive intestinal peptide, cholecystokinin, and somatostatin. Other contributions provide insight into applications of the technique to studies of the expression of genes for neurotransmitter synthesizing enzymes, viral-encoded genes, trophic factor genes, and the genes selected on the basis of their special roles in the brain. The authors provide an important series of technical perspectives, and describe specific experimental protocols. This volume should be of interest to individuals seeking an introduction to these methods, as well to those desiring an up to date precis of work in this burgeoning area. Dr. Uhl, with the sponsorship of the Howard Hughes Medical Institute, has done a superb job of assembling the leaders in this area, and in organizing the presen ta tion of their perspecti ves herein. Joseph B. Martin, M.D., Ph.D.
In Situ Hybridization Techniques for the Brain
In Situ Hybridization Techniques for the Brain - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook In Situ Hybridization Techniques for the Brain write by Z. Henderson. This book was released on 1996-12-11. In Situ Hybridization Techniques for the Brain available in PDF, EPUB and Kindle. Written by molecular biologists and neurobiologists, this volume in the Handbook Series provides basic step-by-step protocols in addition to specialist techniques to support those practising in situ hybridization, a powerful histochemical technique which is now widely used in neurobiology. The first part of the manual concentrates on basic procedures, eg, which probe and label to select, equipment, relevant molecular biological techniques, hazards, probe preparation and labelling, tissue preparation, and conditions for hybridization. Specialist procedures including quantitative analysis of data, reverse transcription PCR, working with human tissue, and double label methods are presented in subsequent sections. In Situ Hybridization Techniques for the Brain is, therefore, an essential reference source for all those with research interests in neuroanatomy, histopathology and/or neurohistochemistry, and will support users in both the preparation and successful completion of the procedures in their own laboratory.
In Situ Hybridization Protocols
In Situ Hybridization Protocols - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook In Situ Hybridization Protocols write by Ian A. Darby. This book was released on 2008-02-04. In Situ Hybridization Protocols available in PDF, EPUB and Kindle. The technique of in situ hybridization, in its various forms, has been used routinely in many laboratories for a number of years. In the post-genome era, gene arrays and proteomics have allowed us to identify hitherto unknown unrecognized pathways and mechanisms. However, rather than diminish the importance of in situ hybridization, the now widespread use of screening te- nologies has increased the need to temporally and spatially localize the dist- bution of mRNA expression. Our intention, in In Situ Hybridization Protocols is to provide ample inf- mation for novices planning to set up the in situ hybridization technique and use it in their laboratory for the first time, as well as giving updates of recent developments for those laboratories where in situ hybridization techniques are already in use. Despite its widespread significance, in situ hybridization has retained a re- tation as one of the more difficult and capricious molecular biological te- niques. This may in part be because of the hybrid nature of the technique, which often requires a mixture of molecular biological and histological skills. The two techniques are usually taught and acquired in different streams of biolo- cal science. The step-by-step and detailed protocols provided in In Situ Hybridization Protocols by researchers active in the field should make it p- sible for both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use the technique s- cessfully in their laboratories.
Brain Development
Brain Development - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Brain Development write by Simon G Sprecher. This book was released on 2016-09-29. Brain Development available in PDF, EPUB and Kindle. Providing widely used techniques in genetic model systems and many complementing animal models, Brain Development: Methods and Protocols focuses its expert contributions on two key technical aspects of developmental neurobiology: detection of gene expression and functional characterization of developmental control genes. Covering animal models such as the fruit fly, zebra fish, chicken, and mouse, this detailed book views in situ hybridization, reporter gene expression, and immunohistochemical staining methods, as well as RNA interference, Morpholino, or transgenic techniques through the prism of these models. Written in the highly successful Methods in Molecular Biology series format, chapter include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Brain Development: Methods and Protocols aims to provide precise technical protocols but also allows for comparing a wide range of protocols in different tissues and species.
