Investigation of Biomolecular Interactions for Development of Sensors and Diagnostics - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Investigation of Biomolecular Interactions for Development of Sensors and Diagnostics write by Xiaojuan Zhang. This book was released on 2011. Investigation of Biomolecular Interactions for Development of Sensors and Diagnostics available in PDF, EPUB and Kindle. The highly specific recognition processes between biomolecules mediate various crucial biological processes. Uncovering the molecular basis of these interactions is of great fundamental and applied importance. This research work focuses on understanding the interactions of several biomolecular recognition systems and processes that can provide fundamental information to aid in the rational design of sensing and molecular recognition tools. Initially, a reliable and versatile platform was developed to investigate biomolecular interactions at a molecular level. This involved several techniques, including biomolecule functionalization to enable attachment to self-assembled monolayers as well as atomic force microscopy (AFM) based force spectroscopy to uncover the binding or rupture forces between the receptor and ligand pairs. It was shown that this platform allowed determination of molecular binding between single molecules with a high specificity. The platform was further adapted to a general sensing formulation utilizing a group of flexible and adaptive nucleic acid recognition elements (RNA and DNA aptamers) to detect specific target proteins. Investigation of interactions at the molecular level allowed characterization of the dynamics, specificity and the conformational properties of these functional nucleic acids in a manner inaccessible via traditional interaction studies. These interactions were then adapted to aptamer-based detecting methods that at the ensemble or bulk scale, specifically taking advantage of mechanisms uncovered in the biophysical study of this system. A quartz crystal microbalance (QCM) was used to detect protein targets at the bulk level and the affinities and binding kinetics of these systems were analyzed. Along with AFM-based force spectroscopy, ensemble-averaging properties and molecular properties of these interactions could be correlated to contribute to bridging the gap across length scales. Finally, more broadly applicable sensing platform was developed to take advantage of the unique properties of aptamers. DNA was employed both as a carrier and as a molecular recognition agent. DNA was used as a template for nanoconstruction and fabricating unique shapes that could enhance the aptamer-based molecular recognition strategies. With aptamers tagged to distinct nanoconstructed DNA, a novel shape-based detecting method was enabled at the molecular level. The results demonstrated that this is a flexible strategy, which can be further developed as ultrasensitive single molecule sensing strategy in complex environments.
Advances in Chemical Bioanalysis
Advances in Chemical Bioanalysis - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Advances in Chemical Bioanalysis write by Frank-Michael Matysik. This book was released on 2014-06-03. Advances in Chemical Bioanalysis available in PDF, EPUB and Kindle. Expert authors provide critical, in-depth reviews of available methods for retrieving selective information out of complex biological systems. Sensors, probes and devices are present and future tools of medicinal diagnostics, environmental monitoring, food analysis and molecular biology. These are based on fluorescence, electrochemistry and mass spectrometry. Coverage of this volume includes sensor development for the detection of small analytes, monitoring of biomolecular interactions, analysis of cellular function, development of diagnostic tools.
Biomolecular Sensors
Biomolecular Sensors - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Biomolecular Sensors write by Electra Gizeli. This book was released on 2002-05-16. Biomolecular Sensors available in PDF, EPUB and Kindle. The development of devices that incorporate biological assemblies is impacting analytical and biomedical research. Today, scientists can monitor vital biological interactions-such as the binding of DNA to proteins-in real time, deriving unique information necessary to understanding biochemical pathways and thus aiding the design of drugs to regulat
Advances in Biosensors
Advances in Biosensors - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Advances in Biosensors write by Bansi Dhar Malhotra. This book was released on 2003-07-09. Advances in Biosensors available in PDF, EPUB and Kindle. There is a worldwide effort towards the development of bioanalytical devices that can be used for detection, quantification and monitoring of specific chemical species. In this context, biosensors represent an emerging trend in the diagnostics industry. A biosensor is a device that has a biological sensing element either intimately connected to or integrated within a transducer. The aim is to produce a digital electronic signal that is proportional to the concentration of a specific chemical or a set of chemicals. Biosensors are specific, rapid, cost-effective and easy to use devices that can be employed with minimal sample treatment. Biosensors have applications in many areas such as biotechnology, healthcare, pollution monitoring, food and agriculture product monitoring, the pharmaceuticals industry and defense. This reference text is devoted to the principles and applications of biosensors and meets the needs of academic institutes, research laboratories and the rapidly developing biosensor industry. Discusses novel ways that can be used to fabricate biosensors for a variety of applications Biosensors have applications in many scientific areas Contributors are experts in their respective fields of research
Sensors for Diagnostics and Monitoring
Sensors for Diagnostics and Monitoring - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Sensors for Diagnostics and Monitoring write by Kevin Yallup. This book was released on 2018-09-03. Sensors for Diagnostics and Monitoring available in PDF, EPUB and Kindle. Sensor technologies and applications are evolving rapidly driven by the demand for new sensors for monitoring and diagnostic purposes to enable improvements in human health and safety. Simultaneously, sensors are required to consume less power, be autonomous, cost less, and be connected by the Internet of Things. New sensor technologies are being developed to fulfill these needs. This book reviews the latest developments in sensor technology and gives the reader an overview of the state-of-the-art in key areas, such as sensors for diagnostics and monitoring. Features Provides an overview of sensor technologies for monitoring and diagnostics applications. Presents state-of-the-art developments in selected topics for sensors that can be used for monitoring and diagnostics in future healthcare, structural monitoring, and smart environment applications. Features contributions from leading international experts in both industry and academia. Explores application areas that include medical diagnostics and screening, health monitoring, smart textiles, and structural monitoring.
