Label-free and Super-resolution Multiphoton Microscopy

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Release : 2017
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Label-free and Super-resolution Multiphoton Microscopy - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Label-free and Super-resolution Multiphoton Microscopy write by Genevieve Vigil. This book was released on 2017. Label-free and Super-resolution Multiphoton Microscopy available in PDF, EPUB and Kindle.

Label-Free Super-Resolution Microscopy

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Release : 2019-08-31
Genre : Science
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Book Rating : 221/5 ( reviews)

Label-Free Super-Resolution Microscopy - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Label-Free Super-Resolution Microscopy write by Vasily Astratov. This book was released on 2019-08-31. Label-Free Super-Resolution Microscopy available in PDF, EPUB and Kindle. This book presents the advances in super-resolution microscopy in physics and biomedical optics for nanoscale imaging. In the last decade, super-resolved fluorescence imaging has opened new horizons in improving the resolution of optical microscopes far beyond the classical diffraction limit, leading to the Nobel Prize in Chemistry in 2014. This book represents the first comprehensive review of a different type of super-resolved microscopy, which does not rely on using fluorescent markers. Such label-free super-resolution microscopy enables potentially even broader applications in life sciences and nanoscale imaging, but is much more challenging and it is based on different physical concepts and approaches. A unique feature of this book is that it combines insights into mechanisms of label-free super-resolution with a vast range of applications from fast imaging of living cells to inorganic nanostructures. This book can be used by researchers in biological and medical physics. Due to its logically organizational structure, it can be also used as a teaching tool in graduate and upper-division undergraduate-level courses devoted to super-resolved microscopy, nanoscale imaging, microscopy instrumentation, and biomedical imaging.

Development of Multiphoton Label-free Super-resolution Microscopy Techniques for Biomedical Imaging

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Release : 2021
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Development of Multiphoton Label-free Super-resolution Microscopy Techniques for Biomedical Imaging - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Development of Multiphoton Label-free Super-resolution Microscopy Techniques for Biomedical Imaging write by Peter Johnson. This book was released on 2021. Development of Multiphoton Label-free Super-resolution Microscopy Techniques for Biomedical Imaging available in PDF, EPUB and Kindle.

Fluorescence Microscopy

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Release : 2014-02-24
Genre : Science
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Book Rating : 136/5 ( reviews)

Fluorescence Microscopy - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Fluorescence Microscopy write by Anda Cornea. This book was released on 2014-02-24. Fluorescence Microscopy available in PDF, EPUB and Kindle. Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers. With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research. Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical research Offers insight into common challenges in implementing techniques, as well as effective solutions

Multiphoton Microscopy and Fluorescence Lifetime Imaging

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Release : 2018-01-22
Genre : Science
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Book Rating : 07X/5 ( reviews)

Multiphoton Microscopy and Fluorescence Lifetime Imaging - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Multiphoton Microscopy and Fluorescence Lifetime Imaging write by Karsten König. This book was released on 2018-01-22. Multiphoton Microscopy and Fluorescence Lifetime Imaging available in PDF, EPUB and Kindle. This monograph focuses on modern femtosecond laser microscopes for two photon imaging and nanoprocessing, on laser tweezers for cell micromanipulation as well as on fluorescence lifetime imaging (FLIM) in Life Sciences. The book starts with an introduction by Dr. Wolfgang Kaiser, pioneer of nonlinear optics and ends with the chapter on clinical multiphoton tomography, the novel high resolution imaging technique. It includes a foreword by the nonlinear microscopy expert Dr. Colin Sheppard. Contents Part I: Basics Brief history of fluorescence lifetime imaging The long journey to the laser and its use for nonlinear optics Advanced TCSPC-FLIM techniques Ultrafast lasers in biophotonics Part II: Modern nonlinear microscopy of live cells STED microscopy: exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensities Principles and applications of temporal-focusing wide-field two-photon microscopy FLIM-FRET microscopy TCSPC FLIM and PLIM for metabolic imaging and oxygen sensing Laser tweezers are sources of two-photon effects Metabolic shifts in cell proliferation and differentiation Femtosecond laser nanoprocessing Cryomultiphoton imaging Part III: Nonlinear tissue imaging Multiphoton Tomography (MPT) Clinical multimodal CARS imaging In vivo multiphoton microscopy of human skin Two-photon microscopy and fluorescence lifetime imaging of the cornea Multiscale correlative imaging of the brain Revealing interaction of dyes and nanomaterials by multiphoton imaging Multiphoton FLIM in cosmetic clinical research Multiphoton microscopy and fluorescence lifetime imaging for resection guidance in malignant glioma surgery Non-invasive single-photon and multi-photon imaging of stem cells and cancer cells in mouse models Bedside assessment of multiphoton tomography