Molecular Methods in Developmental Biology

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Release : 2008-02-03
Genre : Science
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Book Rating : 789/5 ( reviews)

Molecular Methods in Developmental Biology - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Molecular Methods in Developmental Biology write by Matt Guille. This book was released on 2008-02-03. Molecular Methods in Developmental Biology available in PDF, EPUB and Kindle. The process whereby a single cell, the fertilized egg, develops into an adult has fascinated for centuries. Great progress in understanding that process, h- ever, has been made in the last two decades, when the techniques of molecular biology have become available to developmental biologists. By applying these techniques, the exact nature of many of the interactions responsible for forming the body pattern are now being revealed in detail. Such studies are a large, and it seems ever-expanding, part of most life-science groups. It is at newcomers to this field that this book is primarily aimed. A number of different plants and animals serve as common model org- isms for developmental studies. In Molecular Methods in Developmental Bi- ogy: Xenopus and Zebrafish, a range of the molecular methods applicable to two of these organisms are described, these are the South African clawed frog, Xenopus laevis, and the zebrafish, Brachydanio rerio. The embryos of both of these species develop rapidly and externally, making them particularly suited to investigations of early vertebrate development. However, both Xenopus and zebrafish have their own advantages and disadvantages. Xenopus have large, robust embryos that can be manipulated surgically with ease, but their pseudotetraploidy and long generation time make them unsuitable candidates for genetics. This disadvantage may soon be overcome by using the diploid Xenopus tropicalis, and early experiments are already underway. The transp- ent embryos of zebrafish render them well-suited for in situ hybridization and immunohistochemistry, and good for observing mutations in genetic screens.

Methods in Molecular Biology: Molecular methods in developmental biology : xenopus and zebrafish

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Author :
Release : 1984
Genre : Molecular biology -- v.236. Plant functional genomics -- v.237. G protein signaling -- v.238. Biopolymer methods in tissue engineering -- v.239. Cell migration in inflammation and immunity -- v.240. Mammalian artificial chromosomes -- v.241. Cell cycle checkpoint control protocols -- v.242. Atomic force microscopy -- v.243. Chiral separations: Methods and protocols -- v.244. Protein purification protocols -- v.245-6. Gene delivery to mammalian cells -- v.247. Drosophila cytogenetics protocols -- v.248. Antibody engineering -- v.249. Cytokine protocols -- v.251. HPLC of peptides and proteins: Methods and protocols -- v.265. RNA interference, editing, and modification -- v.274. Photosynthesis research protocols -- v.318. Plant cell culture protocols -- v.323. Arabidopsis protocols
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Methods in Molecular Biology: Molecular methods in developmental biology : xenopus and zebrafish - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Methods in Molecular Biology: Molecular methods in developmental biology : xenopus and zebrafish write by John M. Walker. This book was released on 1984. Methods in Molecular Biology: Molecular methods in developmental biology : xenopus and zebrafish available in PDF, EPUB and Kindle.

Cellular and Molecular Procedures in Developmental Biology

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Release : 1997-11-17
Genre : Science
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Book Rating : 594/5 ( reviews)

Cellular and Molecular Procedures in Developmental Biology - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Cellular and Molecular Procedures in Developmental Biology write by . This book was released on 1997-11-17. Cellular and Molecular Procedures in Developmental Biology available in PDF, EPUB and Kindle. This volume incorporates practical methods at the level of molecular, cellular, and whole organism biology in vertebrate and invertebrate models. It presents straightforward protocols written step-by-step for state-of-the-art techniques with the emphasis on single-cell resolution procedures. Provides straightforward, current protocols and critical appraisals Includes diverse analysis of cellular and molecular techniques Presents everything from whole-organ cultures to electrophysiological approaches Details a variety of methods for interfering with gene function in various species Offers multiple illusions of in situ hybridization, immunostaining, and apoptosis

Molecular Embryology

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Release : 2008-02-02
Genre : Science
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Book Rating : 708/5 ( reviews)

Molecular Embryology - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Molecular Embryology write by Paul T. Sharpe. This book was released on 2008-02-02. Molecular Embryology available in PDF, EPUB and Kindle. Most people have some interest in embryos; this probably results, in part, from their interest in understanding the biological origins of themselves and their offspring and, increasingly, concerns about how environmental change such as pollution might affect human development. Obviously, et- cal considerations preclude experimental studies of human embryos and, c- sequently, the developmental biologist has turned to other species to examine this process. Fortunately, the most significant conclusion to be drawn from the experimental embryology of the last two decades is the manner in which orthologous or closely related molecules are deployed to mediate similar - velopmental processes in both vertebrates and invertebrates. The molecular mechanisms regulating processes fundamental to most animals, such as axial patterning or axon guidance, are frequently conserved during evolution. (It is now widely believed that the differences between phyla and classes are the result of new genes, arising mostly by duplication and divergence of extant sequences, regulating the appearance of derived characters. ) Other vertebrates are obviously most likely to use the same devel- mental mechanisms as humans and, within the vertebrate subphylum, the - parent degree of conservation of developmental mechanism is considerable. It has long been recognized that particular vertebrate species offer either d- tinct advantages in investigating particular stages of development or are - pecially amenable to particular manipulations. No single animal can provide all the answers because not all types of experiments can be carried out on a single species.

Molecular Techniques and Approaches in Developmental Biology

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Release : 1973
Genre : Medical
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Molecular Techniques and Approaches in Developmental Biology - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Molecular Techniques and Approaches in Developmental Biology write by Maarten J. Chrispeels. This book was released on 1973. Molecular Techniques and Approaches in Developmental Biology available in PDF, EPUB and Kindle.