Multiphoton Microscopy and Fluorescence Lifetime Imaging - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Multiphoton Microscopy and Fluorescence Lifetime Imaging write by Karsten König. This book was released on 2018-01-22. Multiphoton Microscopy and Fluorescence Lifetime Imaging available in PDF, EPUB and Kindle. This monograph focuses on modern femtosecond laser microscopes for two photon imaging and nanoprocessing, on laser tweezers for cell micromanipulation as well as on fluorescence lifetime imaging (FLIM) in Life Sciences. The book starts with an introduction by Dr. Wolfgang Kaiser, pioneer of nonlinear optics and ends with the chapter on clinical multiphoton tomography, the novel high resolution imaging technique. It includes a foreword by the nonlinear microscopy expert Dr. Colin Sheppard. Contents Part I: Basics Brief history of fluorescence lifetime imaging The long journey to the laser and its use for nonlinear optics Advanced TCSPC-FLIM techniques Ultrafast lasers in biophotonics Part II: Modern nonlinear microscopy of live cells STED microscopy: exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensities Principles and applications of temporal-focusing wide-field two-photon microscopy FLIM-FRET microscopy TCSPC FLIM and PLIM for metabolic imaging and oxygen sensing Laser tweezers are sources of two-photon effects Metabolic shifts in cell proliferation and differentiation Femtosecond laser nanoprocessing Cryomultiphoton imaging Part III: Nonlinear tissue imaging Multiphoton Tomography (MPT) Clinical multimodal CARS imaging In vivo multiphoton microscopy of human skin Two-photon microscopy and fluorescence lifetime imaging of the cornea Multiscale correlative imaging of the brain Revealing interaction of dyes and nanomaterials by multiphoton imaging Multiphoton FLIM in cosmetic clinical research Multiphoton microscopy and fluorescence lifetime imaging for resection guidance in malignant glioma surgery Non-invasive single-photon and multi-photon imaging of stem cells and cancer cells in mouse models Bedside assessment of multiphoton tomography
Multiphoton Microscopy and Fluorescence Lifetime Imaging
Multiphoton Microscopy and Fluorescence Lifetime Imaging - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Multiphoton Microscopy and Fluorescence Lifetime Imaging write by Karsten König. This book was released on 2018-01-22. Multiphoton Microscopy and Fluorescence Lifetime Imaging available in PDF, EPUB and Kindle. This monograph demonstrates the latest developments in two-photon fluorescence microscopy and second-harmonic generation (SHG) microscopy, including coverage of high-resolution microscopy methods, such as STED microscopy. A special focus lies on clinical applications of these methods, e.g. in dermatology, ophtalmology, neuro sciences and cell biology.
Multiphoton Microscopy and Fluorescence Lifetime Imaging
Multiphoton Microscopy and Fluorescence Lifetime Imaging - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Multiphoton Microscopy and Fluorescence Lifetime Imaging write by Karsten König. This book was released on 2016. Multiphoton Microscopy and Fluorescence Lifetime Imaging available in PDF, EPUB and Kindle.
Multiphoton and Fluorescence Lifetime Imaging Microscopy in Studying Nanoparticle Pharmacokinetics in Skin and Liver
Multiphoton and Fluorescence Lifetime Imaging Microscopy in Studying Nanoparticle Pharmacokinetics in Skin and Liver - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Multiphoton and Fluorescence Lifetime Imaging Microscopy in Studying Nanoparticle Pharmacokinetics in Skin and Liver write by Camilla A. Thorling. This book was released on 2016. Multiphoton and Fluorescence Lifetime Imaging Microscopy in Studying Nanoparticle Pharmacokinetics in Skin and Liver available in PDF, EPUB and Kindle. The use of nanoparticles has increased in consumer products in recent decades; however, concerns regarding their safety remain. Zinc oxide is used in sunblocking and may generate free radicals in response to UV illumination, leading to DNA damage and an immunological response. With high-resolution, high-contrast imaging in biological tissue, multiphoton microscopy is able to separate nanoparticles signals from endogenous fluorophores. It has been proven to be very useful in imaging penetration of zinc oxide nanoparticles in skin and in combination with fluorescence lifetime imaging microscopy study cellular function as well. This chapter aims to review the use of these imaging techniques in studying the uptake and distribution of nanoparticles in skin and liver. Due to the questionable clinical use and possible toxicity of nanoparticles, it is important to study their pharmacokinetics. Some nanomaterials have been identified as relatively toxic to humans and a few metal nanoparticles have been reported to penetrate and be detected in blood. Multiphoton microscopy has high resolution and is able to visualize nanoparticles, due to their optical properties, in vivo. The addition of fluorescence lifetime imaging makes it possible to measure the physiochemical environment, with outputs that can be statistically analyzed, posing an advantage over fluorescence intensity imaging only.
Multiphoton Microscopy in the Biomedical Sciences
Multiphoton Microscopy in the Biomedical Sciences - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Multiphoton Microscopy in the Biomedical Sciences write by . This book was released on 2007. Multiphoton Microscopy in the Biomedical Sciences available in PDF, EPUB and Kindle.
