Nuclear Transfer Protocols - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Nuclear Transfer Protocols write by Paul J. Verma. This book was released on 2008-02-02. Nuclear Transfer Protocols available in PDF, EPUB and Kindle. Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The emphasis here is on providing readily reproducible techniques for the gene- tion of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer and serial nuclear transfer. Of immense practical benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones. Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis also provides an understanding of the factors involved in nuclear reprogramming, which is imperative for the success of cloning. A section dealing with such cloning-related issues as aging and normality of clones is also included making this an essential comprehensive handbook for research and commercial labo- tories involved in, or intending to work on, nuclear transfer. The volume will prove beneficial to molecular biologists, stem cell biologists, clinicians, biotechnologists, students, veterinarians, and animal care technicians involved with reprogramming, nuclear transfer, and transgenesis.
Nuclear Transfer Protocols
Nuclear Transfer Protocols - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Nuclear Transfer Protocols write by Paul J. Verma. This book was released on 2006. Nuclear Transfer Protocols available in PDF, EPUB and Kindle. "Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis" is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The emphasis here is on providing readily reproducible techniques for the generation of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer and post-fusion enucleation nuclear transfer.
Nuclear Reprogramming
Nuclear Reprogramming - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Nuclear Reprogramming write by Kejin Hu. This book was released on 2021. Nuclear Reprogramming available in PDF, EPUB and Kindle. This volume provides basic and advanced protocols on somatic cell nuclear transfer, induced pluripotent stem cells, and direct reprogramming of somatic cells into different functional cells. Chapters guide readers through methods on standardized procedures for characterization of induced pluripotent stem cells, as well as those for preparation of materials required for induction of pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Nuclear Reprogramming: Methods and Protocols aims to ensure successful results in the further study of this vital field. .
Nuclear Reprogramming
Nuclear Reprogramming - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Nuclear Reprogramming write by Steve Pells. This book was released on 2008-02-04. Nuclear Reprogramming available in PDF, EPUB and Kindle. A wide-ranging collection of readily reproducible methods for performing nuclear reprogramming by nuclear transfer in several different species, by fusion through both chemical treatment and electrically shocking cells, and by in vivo treatment of cells with cell extracts. Several methods of monitoring nuclear reprogramming are also presented, including the use of transgenic markers, activation of telomerase as an ES-specific marker, light and electron microscopic observation of structural changes in the nucleus, and verification of surface marker expression and the differentiation potential of stem cells. Biochemical methods are provided for the examination of chromatin protein modifications, nucleosomal footprinting, transcription factor binding, and the study of DNA methylation changes both at the specific locus level and at the level of the whole nucleus.
Somatic Cell Nuclear Transfer Technology
Somatic Cell Nuclear Transfer Technology - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Somatic Cell Nuclear Transfer Technology write by Marcelo Tigre Moura. This book was released on 2023-04-11. Somatic Cell Nuclear Transfer Technology available in PDF, EPUB and Kindle. This volume details protocols on animal cloning by Somatic cell nuclear transfer for basic research and biotechnological applications. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Somatic Cell Nuclear Transfer Technology aims to be comprehensive guide for researchers.
