Oligonucleotide Synthesis - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Oligonucleotide Synthesis write by Piet Herdewijn. This book was released on 2008-02-04. Oligonucleotide Synthesis available in PDF, EPUB and Kindle. A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.
Synthesis of Therapeutic Oligonucleotides
Synthesis of Therapeutic Oligonucleotides - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Synthesis of Therapeutic Oligonucleotides write by Satoshi Obika. This book was released on 2018-12-08. Synthesis of Therapeutic Oligonucleotides available in PDF, EPUB and Kindle. This book presents the latest knowledge on a broad range of topics relating to the synthesis of natural and artificial oligonucleotides with therapeutic potential. Nucleic acid-based therapeutics are attracting much attention, and numerous therapeutic oligonucleotides, such as antisense oligonucleotides, siRNAs, splice-switching oligonucleotides, and nucleic acid aptamers, are being evaluated in clinical trials for the treatment of a variety of diseases. Synthesis of Therapeutic Oligonucleotides covers a broad range of topics in the field that are of high relevance to researchers, including the synthesis of natural and chemically modified oligonucleotides, the development of novel nucleic acid analogs, industrial scale synthesis and purification of oligonucleotides, and important aspects of chemistry, manufacturing, and controls (CMC). The aim is to provide new insights and inspire fresh ideas in nucleic acid chemistry that may ultimately lead to novel concepts and techniques and the discovery of more effective nucleic acid drugs. The book will be of high value for both established researchers in the field and students intending to specialize in nucleic acid chemistry research.
Oligonucleotide Synthesis
Oligonucleotide Synthesis - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Oligonucleotide Synthesis write by Michael J. Gait. This book was released on 1984. Oligonucleotide Synthesis available in PDF, EPUB and Kindle.
Protocols for Oligonucleotides and Analogs
Protocols for Oligonucleotides and Analogs - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Protocols for Oligonucleotides and Analogs write by Sudhir Agrawal. This book was released on 1993-08-31. Protocols for Oligonucleotides and Analogs available in PDF, EPUB and Kindle. When first conceived, not only was the aim of Protocols for Oligo nucleotides and Analogs to provide wide coverage of the ohgonuc- otide chemistry field for readers who are well versed within the field, but also to give investigators just entering into the field a new perspective. The very first book on this topic was edited and published by Michael Gait in 1984, in whose laboratory I encountered the newer aspects of oligonucleotide chemistry. Since then, oligonucleotide research has developed to such an extent that its uses extend far beyond basic studies, and now find wide application throughout clinical science as well. Until recently, the major application of oligonucleotides has been in the area of DNA-based diagnostic and "antisense oligonucleotid- based therapeutic approaches. However, oligonucleotides are now also being used as therapeutic agents and are thus frequently found in clinical trials in humans. Synthesis of unmodified oligonucleotides using automated synthe sizers has become a common practice in numerous laboratories. How ever, improvements on the existing techniques and the introduction of ever newer methods for oligonucleotide synthesis is constantly driving ahead in the leading research laboratories. And several new oligonucle otide analogs have been synthesized and studied for their individual prop erties in recent years. The present volume strives to bring the readers the most up-to-date information on the newest aspects of synthesis of oligo nucleotides and their analogs. A separate volume covers synthesis of oligonucleotide conjugates, along with most of the analytical techniques presently used for analysis of oligonucleotides.
Oligonucleotides and Analogues
Oligonucleotides and Analogues - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Oligonucleotides and Analogues write by Fritz Eckstein. This book was released on 1991. Oligonucleotides and Analogues available in PDF, EPUB and Kindle. Interest in oligonucleotide synthesis has expanded dramatically since the publication of M. Gait's book in this series in 1984. The process has been automated, new reagents have been developed, and the range of uses for the compounds produced has increased and continues to grow. This volume provides practical guidance on oligonucleotide synthesis and methods for introducing modifications into these molecules. State- of-the-art techniques for automated synthesis are covered in two chapters on the production of oligodeoxynucleotides and oligoribonucleotides. Synthesis of modified oligodeoxynucleotides is also detailed, including modification of the phosphate backbone to produce phosphorothioates, phosphorodithiolates and methyl phosphonates, all of which are of considerable importance due to thier potential therapeutic applications. Other chapters decsribe production of sugar-modified ilgodeoxynucleotides and the attachment of various reported groups; these techniques are useful to those interested in non-radioactive probes for hybridization and for the study of DNA-DNA and DNA protein interactions. This book will be of interest to academic and industrial researchers, enabling both chemists and non-chemists to synthesize oligonucleotides and analogues for a wide variety of experimental purposes.
