Plant Cell Electroporation And Electrofusion Protocols - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Plant Cell Electroporation And Electrofusion Protocols write by Jac A. Nickoloff. This book was released on 2008-02-02. Plant Cell Electroporation And Electrofusion Protocols available in PDF, EPUB and Kindle. Gene transfer is an essential technology for improving our under standing of gene structure and function. Although there are many meth ods by which DNA may be introduced into cells—including heat and chemical treatments, and microinjection—electroporation has been found to be the most versatile gene transfer technique. Electroporation is effective with a wide variety of cell types, including those that are difficult to transform by other means. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. The early and broad success of electric field-medi ated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, in cluding RNA, enzymes, antibodies, and analytic dyes. The first section of Plant Cell Electroporation and Electrofusion Protocols includes two chapters that serve as a guide to theoretical and practical aspects of electroporation, and will be of particular interest to those developing protocols for as yet untested species or cell types, and a third chapter that describes commercially available electroporation instruments. The remaining chapters describe well-tested protocols for DNA electrotransfection, electroporation of other biomolecules, or cell electrofusion. These chapters also include brief discussions of alterna tives to electric field-based methods, citing the advantages and limita tions of the various methods for achieving specific goals.
Guide to Electroporation and Electrofusion
Guide to Electroporation and Electrofusion - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Guide to Electroporation and Electrofusion write by Donald C. Chang. This book was released on 2012-12-02. Guide to Electroporation and Electrofusion available in PDF, EPUB and Kindle. Electroporation is an efficient method to introduce macromolecules such as DNA into a wide variety of cells. Electrofusion results in the fusion of cells and can be used to produce genetic hybrids or hybridoma cells. Guide to Electroporation and Electrofusion is designed to serve the needs of students, experienced researchers, and newcomers to the field. It is a comprehensive manual that presents, in one source, up-to-date, easy-to-follow protocols necessary for efficient electroporation and electrofusion of bacteria, yeast, and plant and animal cells, as well as background information to help users optimize their results through comprehension of the principles behind these techniques. Covers fundamentals of electroporation and electrofusion in detail: Molecular events, Mechanisms, Kinetics, Gives extensive practical information, The latest applications, Controlling parameters to maximize efficiency, Available instrumentation Presents applications of electroporation and electrofusion in current research situations State-of-the-art modifications to electrical pulses and generators Application of electroporation and electrofusion to unique, alternative cell and tissue types Gives straightforward, detailed, easy-to-follow protocols for Formation of human hybridomas Introduction of genetic material into plant cells and pollen Transfection of mammalian cells Transformation of bacteria, plants, and yeast Production of altered embryos Optimization of electroporation by using reporter genes Comprehensive and up-to-date Convenient bench-top format Approximately 125 illustrations complement the text Complete references with article titles Written by leading authorities in electroporation and electrofusion
Methods in Molecular Biology: Plant cell electroporation and electrofusion protocols.nd protocols
Author : John M. Walker
Release : 1984
Genre : Molecular biology -- v.236. Plant functional genomics -- v.237. G protein signaling -- v.238. Biopolymer methods in tissue engineering -- v.239. Cell migration in inflammation and immunity -- v.240. Mammalian artificial chromosomes -- v.241. Cell cycle checkpoint control protocols -- v.242. Atomic force microscopy -- v.243. Chiral separations: Methods and protocols -- v.244. Protein purification protocols -- v.245-6. Gene delivery to mammalian cells -- v.247. Drosophila cytogenetics protocols -- v.248. Antibody engineering -- v.249. Cytokine protocols -- v.251. HPLC of peptides and proteins: Methods and protocols -- v.265. RNA interference, editing, and modification -- v.274. Photosynthesis research protocols -- v.318. Plant cell culture protocols -- v.323. Arabidopsis protocols
Kind :
Book Rating : /5 ( reviews)
Methods in Molecular Biology: Plant cell electroporation and electrofusion protocols.nd protocols - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Methods in Molecular Biology: Plant cell electroporation and electrofusion protocols.nd protocols write by John M. Walker. This book was released on 1984. Methods in Molecular Biology: Plant cell electroporation and electrofusion protocols.nd protocols available in PDF, EPUB and Kindle.
Animal Cell Electroporation and Electrofusion Protocols
Animal Cell Electroporation and Electrofusion Protocols - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Animal Cell Electroporation and Electrofusion Protocols write by Jac A. Nickoloff. This book was released on 2008-02-03. Animal Cell Electroporation and Electrofusion Protocols available in PDF, EPUB and Kindle. The ability to introduce macromolecules into animal cells, includ ing DNA, RNA, proteins, and other bioactive compounds has facili tated a broad range of biological studies, from biochemistry and biophysics to molecular biology, cell biology, and whole animal stud ies. Gene transfer technology in particular will continue to play an essential role in studies aimed at improving our understanding of the relationships between the gene structure and function, and it has impor tant practical applications in both biotechnology and biomedicine, as evidenced by the current intense interest in gene therapy. Although DNA and other macromolecules may be introduced into cells by a variety of methods, including chemical treatments and microinjection, el- troporation has proven to be simpler to perform, more efficient, and effective with a wider variety of cell types than other techniques. The early and broad success of electric field-mediated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, including RNA, enzymes, antibodies, and analytic dyes. Animal Cell Electroporation and Electrofusion Protocols begins with three chapters that describe the theoretical and practical aspects of electroporation, including a review of the commercially available instrumentation. These introductory chapters will be of particular inter est to those new to electric field technologies and to those developing protocols for as yet untested species or cell types. Nineteen chapters follow that present well-tested protocols for electroporation of proteins and DNA into insect, fish, and mammalian cells.
Animal Cell Electroporation and Electrofusion Protocols. Methods in Molecular Biology
Animal Cell Electroporation and Electrofusion Protocols. Methods in Molecular Biology - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Animal Cell Electroporation and Electrofusion Protocols. Methods in Molecular Biology write by Jac A. Nickoloff. This book was released on 1995. Animal Cell Electroporation and Electrofusion Protocols. Methods in Molecular Biology available in PDF, EPUB and Kindle. This superb collection of well-tested protocols for the electroporation of proteins and DNA into insect, fish, and mammalian cells also includes proven animal cell electrofusion techniques for studies of somatic cell genetics and development, and for generating monoclonal antibodies. It is distinguished by its coverage of important model cell types from many organisms and tissue types and its detailed instructions for the growth and preparation of specific cells to achieve optimum transfection. Its extensive reference lists, citations of alternative transfer methods, advice on pitfalls to avoid, and descriptions of expected results ensure readily reproducible results even for beginners.
