Author : Franck Pellestor
Release : 2007
Genre : Science
Kind :
Book Rating : 134/5 ( reviews)

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PRINS and PNA Technologies in Chromosomal Investigations - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook PRINS and PNA Technologies in Chromosomal Investigations write by Franck Pellestor. This book was released on 2007. PRINS and PNA Technologies in Chromosomal Investigations available in PDF, EPUB and Kindle. Book & CD. Advances in molecular biotechnology have greatly improved the sensitivity and the efficiency of methods utilised for genetic investigations and diagnosis. In the domain of chromosome analysis, the introduction of molecular techniques has led to the development of a new approach, called Molecular Cytogenetics, which has surpassed previously available techniques to become a foremost biological method. The fluorescence in situ hybridisation (FISH) is quickly became the standard technique for in situ chromosomal investigations, as illustrated by its large variety of applications in research and diagnosis. However, during the last decade, alternative methods to FISH have been introduced and have shown to be valuable in detecting chromosomes and quantifying chromosomal abnormalities. These alternative procedures are the Primed IN Situ (PRINS) labelling and the Peptide Nucleic Acid (PNA) probes. The two procedures present several advantages for the in situ detection of nucleic acid sequences, such as the small size of PNA probes and PRINS primers, or the fast kinetics of PRINS and PNA labelling reactions, that make them very attractive for a number of cytogenetic purposes. This book provides a valuable introduction and overview of the principles and the applications of alternative approaches in the field of molecular cytogenetics.