Practice of High Performance Liquid Chromatography

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Release : 2012-12-06
Genre : Science
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Book Rating : 257/5 ( reviews)

Practice of High Performance Liquid Chromatography - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Practice of High Performance Liquid Chromatography write by Heinz Engelhardt. This book was released on 2012-12-06. Practice of High Performance Liquid Chromatography available in PDF, EPUB and Kindle. During its short 20 year history High Performance Liquid Chro matography (HPLC) has won itself a firm place amongst the instrumental methods of analysis. HPLC has caused a revolution in biological and pharmaceutical chemistry. Approximately two thirds of the publications on HPLC are concerned with problems from this area of life science. Biotechnology, where it is necessary to isolate substances from complicated mixtures, is likely to give further impetus to the dissemination of modern liquid chromatog raphy in columns, particularly on the preparative scale. This book presents, by means of examples, the application of HPLC to various fields, as well as fundamental discussions of chromatographic methods. The quality of the analytical result is decisively dependent on the qualities of the equipment employed (by Colin, Guiochon, and Martin). Especially the demands are discussed that are placed on the components of the instrument including those for data acquisition and processing. The section on "quantitative analy sis" (by ABhauer, Ullner) covers besides the principles also the problems of ensuring the quality of the data in detail. The basic problems arising by enlarging the sample size to preparative di mensions and the requirements put on the aparatus are discussed in the section on "preparative applications" (by Wehrli).

High Performance Liquid Chromatography

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Release : 1995-09-30
Genre : Science
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Book Rating : 762/5 ( reviews)

High Performance Liquid Chromatography - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook High Performance Liquid Chromatography write by W.J. Lough. This book was released on 1995-09-30. High Performance Liquid Chromatography available in PDF, EPUB and Kindle. High performance liquid chromatography (HPLC) has long been recognized as one of the most useful and versatile analytical techniques. It has now progressed from being a highly expensive method of analysis to a routine technique with wide applications. Consequently there is a requirement in many chemistry and chemistry-related courses for students to acquire a detailed understanding of the principles and practice of HPLC. Written in a manner suitable for undergraduate students studying analytical chemistry and learning about chromatographic analytical techniques applied to pharmaceutical analysis, biochemistry and related disciplines, High-performance Liquid Chromatography: Fundamental Principles and Practice introduces the fundamentals of HPLC. Loosely structured in three parts, the text begins with a thorough introduction of the subject and then progresses through the essential knowledge of the instrumentation needed for HPLC. The final part covers with the applications of HPLC in real-world situations. Developed by a team of international experts from a wide cross-section of disciplines, the text is relevant to a wide range of courses.

High-performance Liquid Chromatography (HPLC)

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Release : 2014
Genre : Chromatographic analysis
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Book Rating : 547/5 ( reviews)

High-performance Liquid Chromatography (HPLC) - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook High-performance Liquid Chromatography (HPLC) write by Yuegang Zuo. This book was released on 2014. High-performance Liquid Chromatography (HPLC) available in PDF, EPUB and Kindle. Includes bibliographical references and index.

High Performance Liquid Chromatography

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Release : 2013-10-22
Genre : Science
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Book Rating : 299/5 ( reviews)

High Performance Liquid Chromatography - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook High Performance Liquid Chromatography write by Mary T. Gilbert. This book was released on 2013-10-22. High Performance Liquid Chromatography available in PDF, EPUB and Kindle. High Performance Liquid Chromatography focuses on the developments, operating techniques, practices, equipment, and packing materials involved in High Performance Liquid Chromatography (HPLC). The book first offers information on basic chromatographic theory, equipment, and the column. Topics include resolution, efficiency, pumps and gradient systems, connectors, detectors, injectors, column packing and testing, packing materials, and coupling of columns. The text also ponders on sample treatment and separation methods, as well as trace analysis, reversed phase chromatography, and selection/optimization conditions. The publication examines adjustment of selectivity by the use of eluent additives and preparative liquid chromatography. Discussions focus on chromatography on dynamically modified oxide gels, metal complexation, crown ethers, ion pair chromatography, materials for preparative chromatography, and separation strategy. The text also reviews the trends in the practice of HPLC and chiral chromatography. The book is a dependable reference for readers interested in High Performance Liquid Chromatography.

Practical High-Performance Liquid Chromatography

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Release : 2010-05-17
Genre : Science
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Book Rating : 183/5 ( reviews)

Practical High-Performance Liquid Chromatography - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Practical High-Performance Liquid Chromatography write by Veronika R. Meyer. This book was released on 2010-05-17. Practical High-Performance Liquid Chromatography available in PDF, EPUB and Kindle. Jump into the HPLC adventure! Three decades on from publication of the 1st German edition of Veronika Meyer's book on HPLC, this classic text remains one of the few titles available on general HPLC aimed at practitioners. New sections on the following topics have been included in this fifth edition: Comparison of HPLC with capillary electrophoresis How to obtain peak capacity van Deemter curves and other coherences Hydrophilic interaction chromatography Method transfer Comprehensive two-dimensional HPLC Fast separations at 1000 bar HPLC with superheated water In addition, two chapters on the instrument test and troubleshooting in the appendix have been updated and expanded by Bruno E. Lendi, and many details have been improved and numerous references added. A completely new chapter is presented on quality assurance covering: Is it worth the effort? Verification with a second method Method validation Standard operating procedures Measurement uncertainty Qualifications, instrument test, and system suitability test The quest for quality Reviews of earlier editions "That this text is written by an expert in both the practice and teaching of HPLC is evident from the first paragraph....not only an enjoyable, fascinating and easy read, but a truly excellent text that has and will serve many teachers, students and practitioners very well." —The Analyst “…provides essential information on HPLC for LC practitioners in academia, industry, government, and research laboratories…a valuable introduction." - American Journal of Therapeutics