PRINS and PNA Technologies in Chromosomal Investigations - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook PRINS and PNA Technologies in Chromosomal Investigations write by Franck Pellestor. This book was released on 2007. PRINS and PNA Technologies in Chromosomal Investigations available in PDF, EPUB and Kindle. Book & CD. Advances in molecular biotechnology have greatly improved the sensitivity and the efficiency of methods utilised for genetic investigations and diagnosis. In the domain of chromosome analysis, the introduction of molecular techniques has led to the development of a new approach, called Molecular Cytogenetics, which has surpassed previously available techniques to become a foremost biological method. The fluorescence in situ hybridisation (FISH) is quickly became the standard technique for in situ chromosomal investigations, as illustrated by its large variety of applications in research and diagnosis. However, during the last decade, alternative methods to FISH have been introduced and have shown to be valuable in detecting chromosomes and quantifying chromosomal abnormalities. These alternative procedures are the Primed IN Situ (PRINS) labelling and the Peptide Nucleic Acid (PNA) probes. The two procedures present several advantages for the in situ detection of nucleic acid sequences, such as the small size of PNA probes and PRINS primers, or the fast kinetics of PRINS and PNA labelling reactions, that make them very attractive for a number of cytogenetic purposes. This book provides a valuable introduction and overview of the principles and the applications of alternative approaches in the field of molecular cytogenetics.
PRINS and In Situ PCR Protocols
PRINS and In Situ PCR Protocols - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook PRINS and In Situ PCR Protocols write by Franck Pellestor. This book was released on 2008-02-03. PRINS and In Situ PCR Protocols available in PDF, EPUB and Kindle. The in situ hybridization and PCR technologies are now well-established molecular techniques for studying chromosomal aneuploidy and rearran- ments, gene localization and expression, and genomic organization. Over the last decade, we have seen increasing applications in these fields. By combining the high sensitivity of the PCR reaction and the cytological localization of target sequences, both PRINS and in situ PCR techniques have provided highly powerful complements to FISH for in situ cellular and molecular investigations. Both these approaches have several advantages in terms of sensitivity and specificity, owing to the use of primers and to the fast kinetics of annealing and elongation reactions in situ. In the first edition of PRINS and In Situ PCR Protocols edited by John R. Gosden, experts in the field presented in detail a variety of applications of PRINS and in situ PCR techniques, in a wide range of clinical conditions. Since the publication of this successful reference book, there have been s- nificant improvements in in situ detection techniques. This completely revised and updated second edition presents a compreh- sive selection of new procedures developed in the field of PRINS and in situ PCR technologies. The book has two sections. Part I, Basic Methodology, contains chapters that provide useful protocols for many variations of PRINS and in situ PCR, including a new fast multicolor PRINS method, and protocols for PRINS detection of unique sequences in situ.
Companion and Complementary Diagnostics
Companion and Complementary Diagnostics - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Companion and Complementary Diagnostics write by Jan Trøst Jørgensen. This book was released on 2019-05-08. Companion and Complementary Diagnostics available in PDF, EPUB and Kindle. Companion and Complementary Diagnostics: From Biomarker Discovery to Clinical Implementation provides readers with in-depth insights into the individual steps in the development of companion diagnostic assays, from the early biomarker discovery phase straight through to final regulatory approval. Further, the clinical implementation of companion diagnostic testing in the clinic is also discussed. As the development of predictive or selective biomarker assays linked to specific drugs is substantially increasing, this book offers comprehensive information on this quickly-evolving area of biomedicine. It is an essential resource for those in academic institutions, hospitals and pharma, and biotech and diagnostic commercial companies. Covers all aspects, from biomarker discovery, to development and regulatory approval Explains the "how to" aspects of companion diagnostics Incorporates information on the entire process, allowing for easier and deeper understanding of the topic
American Book Publishing Record
American Book Publishing Record - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook American Book Publishing Record write by . This book was released on 2005. American Book Publishing Record available in PDF, EPUB and Kindle.
Fluorescence In Situ Hybridization (FISH) - Application Guide
Fluorescence In Situ Hybridization (FISH) - Application Guide - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Fluorescence In Situ Hybridization (FISH) - Application Guide write by Thomas Liehr. This book was released on 2008-11-26. Fluorescence In Situ Hybridization (FISH) - Application Guide available in PDF, EPUB and Kindle. This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of them are presented in 36 chapters by authors from ten different countries around the world. The book not only provides the reader with basic and background knowledge on the topic, but also gives detailed protocols that show how molecular cytogenetics is currently performed by specialists in this field. The FISH Application Guide initially provides an overview of the (historical) development of molecular cytogenetics, its basic procedures, the equipment required, and probe generation. The book then describes tips and tricks for making different tissues available for molecular cytogenetic studies. These are followed by chapters on various multicolor FISH probe sets, their availability, and their pot- tial for use in combination with other approaches. The possible applications that are shown encompass the characterization of marker chromosomes, cryptic cytogenetic aberrations and epigenetic changes in humans by interphase and metaphase cyto- netics, studies of nuclear architecture, as well as the application of molecular cytogenetics to zoology, botany and microbiology.
