Protein Engineering by Semisynthesis - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Protein Engineering by Semisynthesis write by Carmichael J.A. Wallace. This book was released on 1999-09-29. Protein Engineering by Semisynthesis available in PDF, EPUB and Kindle. As genomics gives way to proteomics as the focus of scientific imagination in the biological sciences, more emphasis will be placed on the technology and interpretation of protein engineering experiments. Protein engineers will become increasingly sophisticated in the questions that they pose and demanding of the tools available to change protein structure. The optimal way to introduce non-coded amino acids for mechanistic studies, or site-specific reporter atoms for spectroscopic structural biology, is by protein semisynthesis. In Protein Engineering by Semisynthesis, the leading practitioners of the method cover their individual protein of expertise forming a comprehensive illustration of the various methods developed. By covering the most recent philosophical and methodological approaches and developments of semisynthesis and peptide synthesis to date, this book provides further understanding of the principles of protein structure-function relationships gained from semisynthetic analog in addition to providing a comprehensive and comprehensible laboratory guide. This book focuses on recent developments which synergistically combine chemical and molecular biological techniques that have made semisynthetic manipulations much easier to undertake. Features
Protein Semisynthesis and Engineering by Expressed Protein Ligation
Protein Semisynthesis and Engineering by Expressed Protein Ligation - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Protein Semisynthesis and Engineering by Expressed Protein Ligation write by Zuzana Machova. This book was released on 2002. Protein Semisynthesis and Engineering by Expressed Protein Ligation available in PDF, EPUB and Kindle.
Protein engineering and other bio-synthetic routes for bio-based materials: Current uses and potential applications
Protein engineering and other bio-synthetic routes for bio-based materials: Current uses and potential applications - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Protein engineering and other bio-synthetic routes for bio-based materials: Current uses and potential applications write by Carissa M Soto. This book was released on 2015-01-22. Protein engineering and other bio-synthetic routes for bio-based materials: Current uses and potential applications available in PDF, EPUB and Kindle. In the past 20 years protein engineering has been used for the production of proteins mostly for biological applications. The incorporation of artificial amino acids and chemical handles into proteins had made possible the design and production of protein-based materials like hybrid inorganic-organic materials, smart/ responsive materials, monodisperse polymers, and nanoscale assemblies. In the current topic, we cover current uses and envision future applications of materials generated using protein engineering and biosynthesis techniques. I would like to acknowledge the U.S. Office of Naval Research for financial support and Dr. Cherise Bernard for her contributions during the early stages of the Research Topic.
Protein Engineering
Protein Engineering - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Protein Engineering write by Saran A. Narang. This book was released on 2013-10-22. Protein Engineering available in PDF, EPUB and Kindle. Protein Engineering: Approaches to the Manipulation of Protein Folding outlines the complexity of the protein-folding problem and the potential of using genetic tools which, in combination with physical techniques, are expected to shed new light. The book begins with an overview of the basic concepts of protein folding, along with prediction methods and protein-folding models. Separate chapters cover experimental approaches to in vitro protein folding; general approaches used to characterize the folding reaction, equilibrium and kinetic experiments; and strategies employed to elucidate structure/function relationships in proteins of unknown tertiary structure. Subsequent chapters cover the structural and functional features of the HIV envelope protein; x-ray diffraction of proteins; application of Fourier transform infrared (FT-IR) spectroscopy to probe the secondary structure and orientation of membrane-associated proteins; and fluorescence measurements of proteins. The final chapters discuss nuclear magnetic resonance studies of proteins and the potential of the synthetic gene approach applied to the problem of protein folding.
Development of In-situ Protein Engineering Technologies for the Study of Modified Proteins
Development of In-situ Protein Engineering Technologies for the Study of Modified Proteins - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Development of In-situ Protein Engineering Technologies for the Study of Modified Proteins write by Michael R. Haugbro. This book was released on 2021. Development of In-situ Protein Engineering Technologies for the Study of Modified Proteins available in PDF, EPUB and Kindle. Deciphering the roles that protein post-translational modifications, protein-protein interactions, and protein dynamics play in modulating protein structure and function requires the ability to introduce amino acid modifications, cross-linking moieties, biophysical probes, and other synthetic chemical species into proteins. Current technologies for the semi-synthesis of modified proteins are optimized for in vitro reactions and are not easily transferable to more biologically relevant systems such as live cell culture. Analogous methodologies that work in situ rely on genetic manipulations that often result in low resolution data and a high level of off-target effects. Early attempts to address this disconnect have employed protein ligase systems such as Sortase and split inteins, but as of yet have not become robust enough for wide-spread use. In this thesis we expand upon the existing technologies and introduce new methods for the precise chemical manipulation of proteins in cell culture using split inteins. We applied in-nucleo protein trans-splicing to determine the in situ interactome of histone PTMs, combining chemical precision and native protein environment in an unprecedented manner unattainable by any other approach. The full results of the proteomics experiments enabled by this technique can be found in Appendix 2, available with the digital version of this thesis. We also expanded the in situ PTS toolbox by developing a multiplexed method for histone labeling in nuclei and in live cells. We demonstrated this capability by labeling two histones with different color fluorophores in a completely orthogonal manner. Finally, we utilized an atypical split intein known as VidaL to achieve the first semi-synthesis of a modified protein in cells, generating histones bearing multiple PTMs and an affinity handle in the chromatin of live cells. We added to this platform by combining our semi-synthesis capabilities with control of the subcellular localization of our target protein, in a single reaction. We envision that these new methods described in this thesis will equip protein chemists and cellular biologists alike with new capabilities for the study of protein structure and function.
