Protein Engineering: Development of a Metal Ion-Dependent Switch - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Protein Engineering: Development of a Metal Ion-Dependent Switch write by Emily S. Kilen. This book was released on 2017. Protein Engineering: Development of a Metal Ion-Dependent Switch available in PDF, EPUB and Kindle. Proteins are biopolymers that perform a myriad of functions in living cells. These functions are determined by each protein's three-dimensional structure. Current knowledge of protein folding principles provides a partial understanding of the thermodynamic factors that drive protein structure, folding, and stability, sufficient to allow proteins to be treated as templates for design and engineering. This project used protein engineering to explore protein structure and folding mediated by interactions with metal ions. As a proof of principle, experiments were undertaken that aimed to re-engineer staphylococcal nuclease to contain a metal ion-dependent switch that exhibits a loss of structure in the absence of a specific metal ion but recovers its native fold in the presence of that ion. Spectroscopic methods were used to monitor structural changes between the metal-free and the metal-bound protein. Changes in the protein's amino acid sequence were introduced systematically to create nickel (II) binding sites based on a naturally occurring high-affinity nickel site. The site was comprised of four amino acid side chains that coordinated the metal ion. Several iterations of candidate proteins, each containing a putative nickel binding site comprised of 2-4 residues, and of reference proteins lacking this site were designed, produced, and characterized spectroscopically. From each of the protein iterations, information was obtained about the refolding process, including the effects of steric constraints, protein oligomerization, and protein thermodynamics. Proteins with fewer substitutions were more effective at maintaining their structure due to a reduced thermodynamic penalty.
Molecular Biology of The Cell
Molecular Biology of The Cell - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Molecular Biology of The Cell write by Bruce Alberts. This book was released on 2002. Molecular Biology of The Cell available in PDF, EPUB and Kindle.
Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics
Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics write by Allan Matte. This book was released on 2020-09-07. Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics available in PDF, EPUB and Kindle. Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics provides the interested and informed reader with an overview of current approaches, strategies and considerations relating to the purification, analytics and characterization of therapeutic antibodies and related molecules. While there are obviously other books published in and around this subject area, they seem to be either older (c.a. year 2000 publication date) or are more limited in scope. The book will include an extensive bibliography of the published literature in the respective areas covered. It is not, however, intended to be a how-to methods book. Covers the vital new area of R&D on therapeutic antibodies Written by leading scientists and researchers Up-to-date coverage and includes a detailed bibliography
Engineering Processes for Bioseparations
Engineering Processes for Bioseparations - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Engineering Processes for Bioseparations write by LAURENCE R. WEATHERLEY. This book was released on 2013-10-22. Engineering Processes for Bioseparations available in PDF, EPUB and Kindle. The use of biotechnology in chemical synthesis offers up numerous advantages to the engineer in the process industries, but it also presents a number of fundamental challenges and difficulties which impinge directly on separation process requirements. The use of biochemical separations has grown significantly during the past decade, and is especially used in process industries such as healthcare and food processing. However it is becoming increasingly more important in areas such as recycling and waste-water treatment and as industry shifts towards cleaner processes biochemical separations will continue to grow. The two main objectives of this book are to focus on the application of existing separation process techniques to the recovery and purification of biologically derived products and to examine the state of knowledge of new techniques which have future potential. Within these objectives the complexities and breadth of problems associated with biological separations are discussed, specific engineering techniques are featured and their adaptation to biochemical separations are highlighted.
Biological Inorganic Chemistry
Biological Inorganic Chemistry - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Biological Inorganic Chemistry write by Ivano Bertini. This book was released on 2007. Biological Inorganic Chemistry available in PDF, EPUB and Kindle. Part A.: Overviews of biological inorganic chemistry : 1. Bioinorganic chemistry and the biogeochemical cycles -- 2. Metal ions and proteins: binding, stability, and folding -- 3. Special cofactors and metal clusters -- 4. Transport and storage of metal ions in biology -- 5. Biominerals and biomineralization -- 6. Metals in medicine. -- Part B.: Metal ion containing biological systems : 1. Metal ion transport and storage -- 2. Hydrolytic chemistry -- 3. Electron transfer, respiration, and photosynthesis -- 4. Oxygen metabolism -- 5. Hydrogen, carbon, and sulfur metabolism -- 6. Metalloenzymes with radical intermediates -- 7. Metal ion receptors and signaling. -- Cell biology, biochemistry, and evolution: Tutorial I. -- Fundamentals of coordination chemistry: Tutorial II.
