A Practical Guide to Protein Engineering

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Release : 2020-10-29
Genre : Science
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Book Rating : 989/5 ( reviews)

A Practical Guide to Protein Engineering - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook A Practical Guide to Protein Engineering write by Tuck Seng Wong. This book was released on 2020-10-29. A Practical Guide to Protein Engineering available in PDF, EPUB and Kindle. This textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a career as a protein engineer. With protein engineering being a fundamental technique in almost all molecular biology labs, the book targets advanced undergraduates and graduate students working in molecular biology, biotechnology and related scientific fields.

Protein Engineering Techniques

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Release : 2016-11-12
Genre : Technology & Engineering
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Book Rating : 323/5 ( reviews)

Protein Engineering Techniques - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Protein Engineering Techniques write by Krishna Mohan Poluri. This book was released on 2016-11-12. Protein Engineering Techniques available in PDF, EPUB and Kindle. This brief provides a broad overview of protein-engineering research, offering a glimpse of the most common experimental methods. It also presents various computational programs with applications that are widely used in directed evolution, computational and de novo protein design. Further, it sheds light on the advantages and pitfalls of existing methodologies and future perspectives of protein engineering techniques.

Protein Engineering Protocols

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Release : 2007-10-26
Genre : Science
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Book Rating : 878/5 ( reviews)

Protein Engineering Protocols - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Protein Engineering Protocols write by Kristian Müller. This book was released on 2007-10-26. Protein Engineering Protocols available in PDF, EPUB and Kindle. Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins. Protein Engineering Protocols will consider the two general, but not mutually exclusive, strategies for protein engineering. The first is known as rational design, in which the scientist uses detailed knowledge of the structure and function of the protein to make desired changes. The s- ond strategy is known as directed evolution. In this case, random mutagenesis is applied to a protein, and selection or screening is used to pick out variants that have the desired qualities. By several rounds of mutation and selection, this method mimics natural evolution. An additional technique known as DNA shuffling mixes and matches pieces of successful variants to produce better results. This process mimics recombination that occurs naturally during sexual reproduction. The first section of Protein Engineering Protocols describes rational p- tein design strategies, including computational methods, the use of non-natural amino acids to expand the biological alphabet, as well as impressive examples for the generation of proteins with novel characteristics. Although procedures for the introduction of mutations have become routine, predicting and und- standing the effects of these mutations can be very challenging and requires profound knowledge of the system as well as protein structures in general.

Protein Engineering and Design

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Release : 1996-06-21
Genre : Science
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Book Rating : 971/5 ( reviews)

Protein Engineering and Design - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Protein Engineering and Design write by Paul R. Carey. This book was released on 1996-06-21. Protein Engineering and Design available in PDF, EPUB and Kindle. The design and production of novel peptides and proteins occupy pivotal positions in science and technology and will continue to do so in the 21st century. Protein Engineering and Design outlines the rapid advances in computer-based modeling, protein engineering, and methods needed for protein and peptide preparation and characterization. This indispensable reference lays the groundwork for understanding this multidisciplinary activity while providing an introduction for researchers and students to the field of protein design. Introduces and defines the techniques involved in protein engineering and design Provides a concise overview of key technologies involved and demonstrates their contributions to the specialized design and production of novel proteins and peptides

Enzymes for Biotechnology

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Release : 1988
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Book Rating : /5 ( reviews)

Enzymes for Biotechnology - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Enzymes for Biotechnology write by . This book was released on 1988. Enzymes for Biotechnology available in PDF, EPUB and Kindle.