Protein Folding and Metal Ions - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Protein Folding and Metal Ions write by Cláudio M. Gomes. This book was released on 2016-04-19. Protein Folding and Metal Ions available in PDF, EPUB and Kindle. The role of metal ions in protein folding and structure is a critical topic to a range of scientists in numerous fields, particularly those working in structural biology and bioinorganic chemistry, those studying protein folding and disease, and those involved in the molecular and cellular aspects of metals in biological systems. Protein Folding an
Protein Engineering: Development of a Metal Ion-Dependent Switch
Protein Engineering: Development of a Metal Ion-Dependent Switch - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Protein Engineering: Development of a Metal Ion-Dependent Switch write by Emily S. Kilen. This book was released on 2017. Protein Engineering: Development of a Metal Ion-Dependent Switch available in PDF, EPUB and Kindle. Proteins are biopolymers that perform a myriad of functions in living cells. These functions are determined by each protein's three-dimensional structure. Current knowledge of protein folding principles provides a partial understanding of the thermodynamic factors that drive protein structure, folding, and stability, sufficient to allow proteins to be treated as templates for design and engineering. This project used protein engineering to explore protein structure and folding mediated by interactions with metal ions. As a proof of principle, experiments were undertaken that aimed to re-engineer staphylococcal nuclease to contain a metal ion-dependent switch that exhibits a loss of structure in the absence of a specific metal ion but recovers its native fold in the presence of that ion. Spectroscopic methods were used to monitor structural changes between the metal-free and the metal-bound protein. Changes in the protein's amino acid sequence were introduced systematically to create nickel (II) binding sites based on a naturally occurring high-affinity nickel site. The site was comprised of four amino acid side chains that coordinated the metal ion. Several iterations of candidate proteins, each containing a putative nickel binding site comprised of 2-4 residues, and of reference proteins lacking this site were designed, produced, and characterized spectroscopically. From each of the protein iterations, information was obtained about the refolding process, including the effects of steric constraints, protein oligomerization, and protein thermodynamics. Proteins with fewer substitutions were more effective at maintaining their structure due to a reduced thermodynamic penalty.
Cadmium: From Toxicity to Essentiality
Cadmium: From Toxicity to Essentiality - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Cadmium: From Toxicity to Essentiality write by Astrid Sigel. This book was released on 2013-02-26. Cadmium: From Toxicity to Essentiality available in PDF, EPUB and Kindle. Volume 11 provides in an authoritative and timely manner in 16 stimulating chapters, written by 40 internationally recognized experts from 11 nations, and supported by more than 2600 references, 35 tables, and over 100 illustrations, many in color, a most up-to-date view on the role of cadmium for life, presently a vibrant research area. MILS-11 covers the bioinorganic chemistry of Cd(II), its biogeochemistry, anthropogenic release into the environment, and speciation in the atmosphere, waters, soils, and sediments. The analytical tools for Cd determination, its imaging in cells, and the use of 113Cd NMR to probe Zn(II) and Ca(II) proteins are summarized, as are Cd(II) interactions with nucleotides, nucleic acids, amino acids, and proteins including metallothioneins. The phytoremediation by Cd(II)-accumulating plants, etc., the toxicology of Cd(II), its damage to mammalian organs, and its role as a carcinogen for humans, are highlighted.
Protein Self-Assembly
Protein Self-Assembly - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Protein Self-Assembly write by Jennifer J. McManus. This book was released on 2020-08-08. Protein Self-Assembly available in PDF, EPUB and Kindle. This volume explores experimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the techniques used to measure protein-protein interactions and equilibrium protein phases in dilute and concentrated protein solutions; Part Two describes methods to measure kinetics of aggregation and to characterize the assembled state; and Part Three details several different computational approaches that are currently used to help researchers understand protein self-assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Protein Self-Assembly: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field.
Protein-Metal Interactions
Protein-Metal Interactions - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Protein-Metal Interactions write by Mendel Friedman. This book was released on 2012-12-06. Protein-Metal Interactions available in PDF, EPUB and Kindle. Metal ions and proteins are ubiquitous. Therefore, not surprisingly, new protein-metal interactions continue to be dis covered, and their importance is increasingly recognized in both physical and life sciences. Because the subject matter is so broad and affects so many disciplines, in organizing this Symposium, I sought participation of speakers with the broadest possible range of interests. Twenty-two accepted my invitation. To supplement the verbal presentations, the Proceedings include five closely re lated invited contributions. The ideas expressed are those of the various authors and are not necessarily approved or rejected by any agency of the United States Government. No official recommendation concerning the sub ject matter or products discussed is implied in this book. This book encompasses many aspects of this multifaceted field. Topics covered represent biochemical, immunochemical, bioorganic, biophysical, metabolic, nutritional, medical, physiological, toxi cological, environmental, textile, and analytical interests. The discoveries and developments in any of these areas inevitably illumine others. I feel that a main objective of this Symposium, bringing together scientists with widely varied experiences yet with common interests in protein-metal interactions, so that new understanding and new ideas would result has been realized. I hope that the reader enjoys and benefits from reading about the fascinat ing interactions of metal ions and proteins as much as I did.
