Quantitative Imaging in Cell Biology - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Quantitative Imaging in Cell Biology write by . This book was released on 2014-06-25. Quantitative Imaging in Cell Biology available in PDF, EPUB and Kindle. This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material
Quantitative and Qualitative Microscopy
Quantitative and Qualitative Microscopy - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Quantitative and Qualitative Microscopy write by P. Michael Conn. This book was released on 2013-10-22. Quantitative and Qualitative Microscopy available in PDF, EPUB and Kindle. Methods in Neurosciences, Volume 3: Quantitative and Qualitative Microscopy is a collection of papers that deals with microscopic techniques in statistical measures. This volume describes microscopy using sophisticated stains and dyes to advance observation of tests and experiments. Section I describes autoradiography including micro chemical methods, high-resolution autoradiography, and single- or double-label quantitative autoradiography for use in imaging of brain activity patterns or determining cerebral physiology. Section II discusses the quantification of structures through statistical and computational methods including dynamic video imaging technology. Section III explains the use of tracers, toxins, or dyes in tracing neuronal connections. One paper addresses the use of small injections of axonally transported fluorescent tracers. Section IV explains staining technology such as using the silver impregnation method for frozen sections of human nervous tissue that are gathered from tissues preserved in formalin. Section V addresses freezing techniques and those using freeze-fracture methods in neurobiology. The text also discusses cryoprotection and other freezing methods to control ice crystals found in fixed or unfixed brain tissues. Section VI presents the combined and high-resolution methods in polarization microscopy and microscopic investigations. Cellular biologists, micro-chemists, and scientific researchers in the field of micro- and cellular biology will appreciate this book.
Coherent Light Microscopy
Coherent Light Microscopy - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Coherent Light Microscopy write by Pietro Ferraro. This book was released on 2011-02-09. Coherent Light Microscopy available in PDF, EPUB and Kindle. This book deals with the latest achievements in the field of optical coherent microscopy. While many other books exist on microscopy and imaging, this book provides a unique resource dedicated solely to this subject. Similarly, many books describe applications of holography, interferometry and speckle to metrology but do not focus on their use for microscopy. The coherent light microscopy reference provided here does not focus on the experimental mechanics of such techniques but instead is meant to provide a users manual to illustrate the strengths and capabilities of developing techniques. The areas of application of this technique are in biomedicine, medicine, life sciences, nanotechnology and materials sciences.
Quantitative Fluorescence Microscopy
Quantitative Fluorescence Microscopy - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Quantitative Fluorescence Microscopy write by F. W. D. Rost. This book was released on 1991-07-26. Quantitative Fluorescence Microscopy available in PDF, EPUB and Kindle. Quantitative fluorescence microscopy is concerned with making measurements from fluorescent specimens in a fluorescence microscope, by measuring fluorescence emission from a defined area or areas of a specimen. This technique is most commonly used to determine the amount of some specific substance, such as DNA, in some particular area of a cell. But it has many other uses; for example, it can be used to identify certain substances in the cell by examining their fluorescence characteristics. This book is a complete guide to this technique for all biologists. It describes the principles and applications of quantitative fluorescence microscopy and also gives much practical information about the instrumentation required. There is also a discussion of the exciting developments in confocal fluorescence microscopy which allows the three dimensional distribution of particular substances to be determined. Everyone presently using this technique, or wishing to start using it will need to read this book.
Methods in Neurosciences
Methods in Neurosciences - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Methods in Neurosciences write by P. Michael Conn. This book was released on 2016-07-02. Methods in Neurosciences available in PDF, EPUB and Kindle. Because of the significance of morphology and cellular communication unique to the functioning and interdependence of neural systems, many of the advances in the neurosciences rely on classic as well as new microscopy techniques. A convenient bench-top format**Methods presented for easy adaptation to new systems**Comprehensive protocols included for**Autoradiography: measurement of hormone receptors, high-resolution techniques, double labeling and combined techniques**Statistical and computational methods: video imaging, three-dimensional reconstructions, means of structural quantitation, unbiased sampling methods, measurement of analysis of neuronal connections, staining, and individual axon arbors**Tracing neuronal connections: tracer, toxin, and dye methods**Staining techniques: fluoro-gold, SITS, and silver impregnation**Freezing techniques: freeze fracture and cryoprotection**Combined and high-resolution techniques: double-label tracer techniques, incident light polarization, light and electron microscopic techniques
