Rationally Designed Proteinogenic Hydrogels as Extracellular Matrix Mimics for 3D Cell Culture - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Rationally Designed Proteinogenic Hydrogels as Extracellular Matrix Mimics for 3D Cell Culture write by . This book was released on 2012. Rationally Designed Proteinogenic Hydrogels as Extracellular Matrix Mimics for 3D Cell Culture available in PDF, EPUB and Kindle.
Rationally Designed Proteinogenic Hydrogels as Extracellular Matrix Mimics for 3D Cell Culture
Rationally Designed Proteinogenic Hydrogels as Extracellular Matrix Mimics for 3D Cell Culture - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Rationally Designed Proteinogenic Hydrogels as Extracellular Matrix Mimics for 3D Cell Culture write by Edgardo Abelardo Dohns. This book was released on 2012. Rationally Designed Proteinogenic Hydrogels as Extracellular Matrix Mimics for 3D Cell Culture available in PDF, EPUB and Kindle. Rationally designed polypeptide-based materials have advantages over other biomaterials for their ability to be engineered from the bottom-up, and to be tailored to the needs of specific potential applications. Based on previous works in the Woolfson laboratory on self- assembling peptide fibres (SAF), this research project focused on developing coiled-coil- based fibrillar hydrogel networks called hydrogelating SAFs (hSAFs), and applying them to more-complex biological systems, notably in cell culture. The hSAFs were tested as a three- dimensional cell-culture scaffold with the aim of mimicking the microenvironment provided by native extracellular matrix (ECM). hSAFs were shown to support growth and differentiation of neuronal cells. Furthermore, a design strategy was explored on how to couple short biomimetic peptides to these proteinogenic scaffolds to enhance cell-matrix interactions. This was achieved by integrating the well-studied cell-adhesion motif RGDS (Arg-Gly-Asp- Ser) to hSAFs using bioorthogonal copper-based click chemistry. Addition of such biomimetic peptides did not alter folding, fibre formation, and higher-order assembly (gelation) of the hSAFs. The resulting functionalised gels promoted early differentiation and longer neurite-like outgrowth in PC12 cells, and of primary rat hippocampal cells. The attached biomimetic peptide was functional and elicited the desired cellular response. Another short biomimetic peptides, i.e. the laminin-derived IKVAV (lIe-Lys-Val-Ala-Val), which supports neurite growth, was also successfully incorporated into hSAFs and gels. Thus, we have demonstrated a chemically defined, bottom-up approach to biological scaffold synthesis and assembly, and further the use of straightforward techniques to incorporate specific biomolecular signals under physiological conditions. Although conditions will need to be optimised for different cell types, hSAF gel formation and functionalisation via click chemistry promise a robust platform in cell-culture studies and potentially for tissue engineering.
Amino Acid
Amino Acid - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Amino Acid write by Toshiki Asao. This book was released on 2017-06-28. Amino Acid available in PDF, EPUB and Kindle. Amino Acid - New Insights and Roles in Plant and Animal provides useful information on new aspects of amino acid structure, synthesis reactions, dietary application in animals, and metabolism in plants. Section 1 includes chapters that describe the therapeutic uses, antiallergic effects, new aspects in the D-amino acid structure, historical background of desmosines, and stereoselective synthesis of ?-aminophosphonic acids. Section 2 presents the role of amino acids in plants, which includes new insights and aspects of D-amino acids, metabolism and transport in soybean, changes during energy storage compound accumulation of microalgae, and determination of amino acids from natural compounds. Section 3 describes the chapters on methodologies and requirement of dietary amino acids for Japanese quails, laying hens, and finishing pigs. The final chapter identifies potential importance of glutathione S-transferase activity for generating resistance to triclabendazole in Fasciola hepatica.
Molecular Gels
Molecular Gels - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Molecular Gels write by Richard G. Weiss. This book was released on 2006-06-30. Molecular Gels available in PDF, EPUB and Kindle. "Molecular Gels: Materials with Self-Assembled Fibrillar Networks" is a comprehensive treatise on gelators, especially low molecular-mass gelators and the properties of their gels. The structures and modes of formation of the self-assembled fibrillar networks (SAFINs) that immobilize the liquid components of the gels are discussed experimentally and theoretically. The spectroscopic, rheological, and structural features of the different classes of low molecular-mass gelators are also presented. Many examples of the application of the principal analytical techniques for investigation of molecular gels (including SANS, SAXS, WAXS, UV-vis absorption, fluorescence and CD spectroscopies, scanning electron, transmission electron and optical microscopies, and molecular modeling) are presented didactically and in-depth, as are several of the theories of the stages of aggregation of individual low molecular-mass gelator molecules leading to SAFINs. Several actual and potential applications of molecular gels in disparate fields (from silicate replication of nanostructures to art conservation) are described. Special emphasis is placed on perspectives for future developments. This book is an invaluable resource for researchers and practitioners either already researching self-assembly and soft matter or new to the area. Those who will find the book useful include chemists, engineers, spectroscopists, physicists, biologists, theoreticians, and materials scientists.
Fmoc Solid Phase Peptide Synthesis
Fmoc Solid Phase Peptide Synthesis - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Fmoc Solid Phase Peptide Synthesis write by W. Chan. This book was released on 1999-12-16. Fmoc Solid Phase Peptide Synthesis available in PDF, EPUB and Kindle. Since the publication of Atherton and Sheppard's volume, the technique of Fmoc solid-phase peptide synthesis has matured considerably and is now the standard approach for the routine production of peptides. The focus of this new volume is much broader, and covers the essential procedures.
