RNA Interference in Practice - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook RNA Interference in Practice write by Ute Schepers. This book was released on 2006-03-06. RNA Interference in Practice available in PDF, EPUB and Kindle. This hands-on guide to RNA interference brings the power of targeted gene silencing to any laboratory with the basic equipment for handling nucleic acids. In easy-to-follow, step-by-step protocols you will learn * how RNAi works in worms, flies and mammals, * how to design the most efficient RNAi constructs, * how to achieve transient, stable and conditional RNAi in cell cultures, * how to determine the efficiency of an RNAi experiment, * and how to use RNAi for gene therapy. All the protocols have been thoroughly tested in the author's own laboratory, and she provides examples of successful experiments and troubleshooting hints to help in establishing your own successful RNAi experiments. Also includes a list of suppliers for RNAi reagents and equipment as well as a glossary of terms.
RNA Interference (RNAi)
RNA Interference (RNAi) - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook RNA Interference (RNAi) write by Wiley. This book was released on 2013-11-26. RNA Interference (RNAi) available in PDF, EPUB and Kindle. The 2006 Nobel Prize in Physiology or Medicine was awarded to Andrew Z. Fire and Craig C. Mello for their discovery of RNA interference (RNAi). RNAi is a biological process in which RNA molecules can silence (inhibit) or up- or down-regulate gene expression, typically by causing the destruction of specific messenger RNA (mRNA) molecules. This collection of articles begins with an introduction to RNAi, and proceeds to describe uses of this technology in various approaches to disease treatment, including gene therapy. Several laboratory protocols for silencing genes via RNAi are also provided, as are protocols for down-regulating and then rescuing those down-regulated genes, which demonstrates specificity of the approach. This e-book — a curated collection from eLS, WIREs, and Current Protocols — offers a fantastic introduction to the field of RNA interference for students or interdisciplinary collaborators.
RNA Interference
RNA Interference - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook RNA Interference write by David R. Engelke. This book was released on 2005-02-28. RNA Interference available in PDF, EPUB and Kindle. The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.RNA Interference will cover RNAi in non-vertebrates (plants, C. elegans, drosophila, and S. pombe), and Mammalian systems (human and non-human cells). This volume discusses extensive methodology related to delivery methods high throughput strategies and prospects as a human therapy agent.* One of the most highly respected publications in the field of biochemistry since 1955 * Frequently consulted, and praised by researchers and reviewers alike * Truly an essential publication for anyone in any field of the life sciences
RNA Interference, Editing, and Modification
RNA Interference, Editing, and Modification - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook RNA Interference, Editing, and Modification write by Jonatha M. Gott. This book was released on 2008-02-05. RNA Interference, Editing, and Modification available in PDF, EPUB and Kindle. This volume presents a comprehensive collection of cuttingedge methods for elucidating the function of new genes and altering gene expression. These readily reproducible techniques can be used either in transient and stable gene splicing applied to worms, flies, trypanosomes, mammals, and plants, or in studying RNA editing mechanisms in a wide range of organisms, including systems that involve the conversion of one base to another and insertion/deletion editing. Topics of interest include stable and transient RNA interference, gene silencing, RNA editing, bioinformatics, small noncoding RNAs, and RNomics. Special attention is given to methods for the identification and characterization of small RNAs involved in RNA interference or modification. Readily reproducible protocols for discovering new genes or altering gene expression.
RNA Interference Technology
RNA Interference Technology - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook RNA Interference Technology write by Krishnarao Appasani. This book was released on 2005-01-17. RNA Interference Technology available in PDF, EPUB and Kindle. RNA Interference (RNAi) technology has rapidly become one of the key methods used in functional genomics. RNAi is used to block the expression of genes and create phenotypes that can potentially yield clues about the function of these genes. In the postgenomic era, the elucidation of the physiological function of genes has become the rate-limiting step in the quest to develop 'gene-based drugs' and RNAi could potentially play a pivotal role in the validation of such novel drugs. In this overview, the basic concepts and applications of RNAi biology are discussed. Leading experts from both academia and industry have contributed to this invaluable reference. The volume is forwarded by Andrew Fire, one of the winners of the 2006 Nobel Prize for the discovery of RNA Interference.
