Single-molecule Tracking and Its Application in Biomolecular Binding Detection - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Single-molecule Tracking and Its Application in Biomolecular Binding Detection write by Cong Liu (Ph. D.). This book was released on 2017. Single-molecule Tracking and Its Application in Biomolecular Binding Detection available in PDF, EPUB and Kindle. In the past two decades significant advances have been made in single-molecule detection, which enables the direct observation of single biomolecules at work in real time under physiological conditions. In particular, the development of single-molecule tracking (SMT) microscopy allows us to monitor the motion paths of individual biomolecules in living systems, unveiling the localization dynamics and transport modalities of the biomolecules that support the development of life. While 3D-SMT is probably the most suitable method for determining whether tracked molecules (can be any biomolecule such as DNA, membrane receptors, and transcription factors) form dimers or complexes with other molecules, great technical challenges remain to be overcome before the potential of 3D-SMT in biomolecular binding detection can be realized. This dissertation describes my work on recent methodology development to overcome these challenges, and new applications of the 3D-SMT technology in rare molecular species quantification. First, we provide an overview of current SMT technologies, with an emphasis on three-dimensional feedback controlled SMT. Advantages and drawbacks of each SMT method are outlined. Second, we describe the theoretical modeling and instrumentation of our confocal tracking microscope. Its multi-dimensional sensing capability (3D position, diffusion coefficient, fluorescence lifetime) is experimentally characterized. In order to maximize the tracking duration, we have also developed strategies to effectively slow-down fast diffusing molecule, and optimized the buffer conditions. Third, we show that our 3D-SMT microscope can detect biomolecular association/disassociated by two types of contrast mechanisms: diffusion rate and lifetime FRET signal. DNA transient binding is used as a model system because of ease of fluorescent labeling and tunable binding kinetics. Both of the two mechanisms involve tracking a fluorescent-labeled single-stranded DNA (ssDNA), but the second approach also requires its complementary strand to be labeled by a dark quencher. A combined analysis of multiple single-molecule trajectories allow us to measure the kinetics that is even beyond the physical bandwidth of the tracking system. In the end, we introduce the application of SMT in rare single-molecule species quantification. The theory for predicting the sensitivity and fidelity is established. Our work highlights the fundamental limitations that we are facing in precise single-molecule identification and quantification without amplification.
Single Biomolecule Detection and Analysis
Single Biomolecule Detection and Analysis - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Single Biomolecule Detection and Analysis write by Tuhin Subhra Santra. This book was released on 2023-08-15. Single Biomolecule Detection and Analysis available in PDF, EPUB and Kindle. This collection discusses various micro/nanodevice design and fabrication for single-biomolecules detection. It will be an ideal reference text for graduate students and professionals in diverse subject areas including materials science, biomedical engineering, chemical engineering, mechanical engineering, and nanoscience. This book- Discusses techniques of single-biomolecule detection, their advantages, limitations, and applications. Covers comprehensively several electrochemical detection techniques. Provides single-molecule separation, sensing, imaging, sequencing, and analysis in detail. Examines different types of cantilever-based biomolecule sensing, and its limitations. Single Biomolecule Detection and Analysis covers single-biomolecule detection and characterization using micro/nanotechnologies and micro/nanofluidic devices, electrical and magnetic detection technologies, microscopy and spectroscopy techniques, single biomolecule optical, and nanopore devices. The text covers key important biosensors-based detection, stochastic optical reconstruction microscopy-based detection, electrochemical detection, metabolic engineering of animal cells, single-molecule intracellular delivery and tracking, terahertz spectroscopy-based detection, total internal reflection fluorescence (TIFR) detection, and Fluorescence Correlation Spectroscopy (FCS) detection. The text will be useful for graduate students and professionals in diverse subject areas including materials science, biomedical engineering, chemical engineering, mechanical engineering, and nanoscience. Discussing chemical process, physical process, separation, sensing, imaging, sequencing, and analysis of single-molecule detection, this text will be useful for graduate students and professionals in diverse subject areas including materials science, biomedical engineering, chemical engineering, mechanical engineering, and nanoscience. It covers microscopy and spectroscopy techniques for single-biomolecule detection, analysis, and their biomedical engineering applications.
Single Molecule Analysis
Single Molecule Analysis - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Single Molecule Analysis write by Iddo Heller. This book was released on 2023-11-13. Single Molecule Analysis available in PDF, EPUB and Kindle. This third edition volume expands on the previous editions with new discussions on the latest techniques and developments in the field. The chapters in this book are organized into four parts, and cover topics such as optical tweezers; single-molecule fluorescence tools; atomic force microscopy; magnetic tweezers; applications to virus protein shells, unfolding of proteins, nucleic acids, motor proteins, in vivo and in vitro; and protocols to establish specific surface interactions and perform force calibration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Single Molecule Analysis: Methods and Protocols, Third Edition is a valuable resource for all researchers who want to learn more about this exciting and still expanding field. Chapters 2, 7, 8, 9, 12, 18, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Single Molecule Spectroscopy
Single Molecule Spectroscopy - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Single Molecule Spectroscopy write by R. Rigler. This book was released on 2012-12-06. Single Molecule Spectroscopy available in PDF, EPUB and Kindle. The topics range from single molecule experiments in quantum optics and solid-state physics to analogous investigations in physical chemistry and biophysics.
Single-molecule Techniques
Single-molecule Techniques - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Single-molecule Techniques write by Paul R. Selvin. This book was released on 2008. Single-molecule Techniques available in PDF, EPUB and Kindle. Geared towards research scientists in structural and molecular biology, biochemistry, and biophysics, this manual will be useful to all who are interested in observing, manipulating and elucidating the molecular mechanisms and discrete properties of macromolecules.
