Somatic Cell Nuclear Transfer Technology - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Somatic Cell Nuclear Transfer Technology write by Marcelo Tigre Moura. This book was released on 2023-08-21. Somatic Cell Nuclear Transfer Technology available in PDF, EPUB and Kindle. This volume details protocols on animal cloning by Somatic cell nuclear transfer for basic research and biotechnological applications. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Somatic Cell Nuclear Transfer Technology aims to be comprehensive guide for researchers.
Somatic Cell Nuclear Transfer
Somatic Cell Nuclear Transfer - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Somatic Cell Nuclear Transfer write by Peter Sutovsky. This book was released on 2007-12-22. Somatic Cell Nuclear Transfer available in PDF, EPUB and Kindle. This book provides the reader with a unique developmental, molecular and cell biological view of the exciting SCNT technology, while also discussing its pitfalls and possible future directions and detours. The cellular events arising from SCNT procedure, such as the need for nuclear remodeling, disposal of donor cell organelles, presence of foreign mitochondrial DNA and altered mode of oocyte activation are explained.
Scientific and Medical Aspects of Human Reproductive Cloning
Scientific and Medical Aspects of Human Reproductive Cloning - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Scientific and Medical Aspects of Human Reproductive Cloning write by National Research Council. This book was released on 2002-06-17. Scientific and Medical Aspects of Human Reproductive Cloning available in PDF, EPUB and Kindle. Human reproductive cloning is an assisted reproductive technology that would be carried out with the goal of creating a newborn genetically identical to another human being. It is currently the subject of much debate around the world, involving a variety of ethical, religious, societal, scientific, and medical issues. Scientific and Medical Aspects of Human Reproductive Cloning considers the scientific and medical sides of this issue, plus ethical issues that pertain to human-subjects research. Based on experience with reproductive cloning in animals, the report concludes that human reproductive cloning would be dangerous for the woman, fetus, and newborn, and is likely to fail. The study panel did not address the issue of whether human reproductive cloning, even if it were found to be medically safe, would beâ€"or would not beâ€"acceptable to individuals or society.
Nuclear Transfer Protocols
Nuclear Transfer Protocols - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Nuclear Transfer Protocols write by Paul J. Verma. This book was released on 2006-07-18. Nuclear Transfer Protocols available in PDF, EPUB and Kindle. Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The emphasis here is on providing readily reproducible techniques for the gene- tion of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer and serial nuclear transfer. Of immense practical benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones. Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis also provides an understanding of the factors involved in nuclear reprogramming, which is imperative for the success of cloning. A section dealing with such cloning-related issues as aging and normality of clones is also included making this an essential comprehensive handbook for research and commercial labo- tories involved in, or intending to work on, nuclear transfer. The volume will prove beneficial to molecular biologists, stem cell biologists, clinicians, biotechnologists, students, veterinarians, and animal care technicians involved with reprogramming, nuclear transfer, and transgenesis.
Principles of Cloning
Principles of Cloning - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Principles of Cloning write by Jose Cibelli. This book was released on 2013-09-24. Principles of Cloning available in PDF, EPUB and Kindle. Principles of Cloning, Second Edition is the fully revised edition of the authoritative book on the science of cloning. The book presents the basic biological mechanisms of how cloning works and progresses to discuss current and potential applications in basic biology, agriculture, biotechnology, and medicine. Beginning with the history and theory behind cloning, the book goes on to examine methods of micromanipulation, nuclear transfer, genetic modification, and pregnancy and neonatal care of cloned animals. The cloning of various species—including mice, sheep, cattle, and non-mammals—is considered as well. The Editors have been involved in a number of breakthroughs using cloning technique, including the first demonstration that cloning works in differentiated cells done by the Recipient of the 2012 Nobel Prize for Physiology or Medicine – Dr John Gurdon; the cloning of the first mammal from a somatic cell – Drs Keith Campbell and Ian Wilmut; the demonstration that cloning can reset the biological clock - Drs Michael West and Robert Lanza; the demonstration that a terminally differentiated cell can give rise to a whole new individual – Dr Rudolf Jaenisch and the cloning of the first transgenic bovine from a differentiated cell – Dr Jose Cibelli. The majority of the contributing authors are the principal investigators on each of the animal species cloned to date and are expertly qualified to present the state-of-the-art information in their respective areas. - First and most comprehensive book on animal cloning, 100% revised - Describes an in-depth analysis of current limitations of the technology and research areas to explore - Offers cloning applications on basic biology, agriculture, biotechnology, and medicine
