Subcellular Fractionation - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Subcellular Fractionation write by J. M. Graham. This book was released on 1997-01-30. Subcellular Fractionation available in PDF, EPUB and Kindle. Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers all the necessary aspects, from breaking up the cells (homogenization), via a variety of separation techniques (the isolation and fractionation chapters), to characterization of the separated organelles.
Molecular Biology of The Cell
Molecular Biology of The Cell - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Molecular Biology of The Cell write by Bruce Alberts. This book was released on 2002. Molecular Biology of The Cell available in PDF, EPUB and Kindle.
Subcellular Fractionation
Subcellular Fractionation - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Subcellular Fractionation write by Paul R. Pryor. This book was released on 2015. Subcellular Fractionation available in PDF, EPUB and Kindle. Eukaryotic cells are remarkably complex structures, containing a vast repertoire of macromolecules, organelles, and other compartments that orchestrate the tasks required for life. For in-depth studies of their function and composition, reliable methods for the isolation of specific subcellular structures are often required. This laboratory manual provides step-by-step protocols for the extraction of subcellular components from animal tissues, yeasts, plants, and cultured cells. Each chapter focuses on a particular eukaryotic organelle, vesicle, membrane, or macromolecular complex. Strategies for breaking cells while maintaining the structural and functional integrity of the component of interest, enriching for that component based on its physical and biochemical characteristics, and monitoring and ensuring the success of the purification procedure are provided. The contributors describe both traditional approaches (e.g., density gradient centrifugation) and innovative techniques (e.g., the use of SPIONs) for isolating subcellular constituents. This manual is therefore an essential laboratory resource for all cell biologists seeking a comprehensive collection of dependable subcellular fractionation methods.
Subcellular Components
Subcellular Components - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Subcellular Components write by G. D. Birnie. This book was released on 2014-05-20. Subcellular Components available in PDF, EPUB and Kindle. Subcellular Components: Preparation and Fractionation talks about cells and particles' components, including their preparation and fractionation. The book includes theories and answers to questions that are relevant to the study. The first chapter of the book details various facts about homogenization of mammalian cells. This chapter presents the results of studies on solid tissues and single-cell suspensions; the author then offers his conclusion of the study. The next two chapters highlight the methods on isolating nuclei, including the guides for standard assessment and the procedure of isolation, along with analysis of nuclei biochemical properties. The main topics in Chapter 4 are mitochondria from animal tissues and yeasts; this chapter also discusses the preparation for a rat-liver, blowfly flight-muscle, yeast, and brain mitochondria. The chapter that follows widely talks about lysosomes, including its historical background, centrifugal method, and related topics. In the next several chapters, the topics covered include purification, isolation, preparation, and separation of cells including plasma-membrane, polysomes, ribosomes, microsomes, and microvilli. The book serves as a great reference for undergraduates and postgraduates in the field, as it contains a thorough discussion of various relevant studies.
Subcellular Fractionation
Subcellular Fractionation - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Subcellular Fractionation write by M. Graham. This book was released on 1997. Subcellular Fractionation available in PDF, EPUB and Kindle. Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers all the necessary aspects, from breaking up the cells (homogenization), via a variety of separation techniques (the isolation and fractionation chapters), to characterization of the separated organelles.
