Synthesis of Therapeutic Oligonucleotides - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Synthesis of Therapeutic Oligonucleotides write by Satoshi Obika. This book was released on 2018-12-08. Synthesis of Therapeutic Oligonucleotides available in PDF, EPUB and Kindle. This book presents the latest knowledge on a broad range of topics relating to the synthesis of natural and artificial oligonucleotides with therapeutic potential. Nucleic acid-based therapeutics are attracting much attention, and numerous therapeutic oligonucleotides, such as antisense oligonucleotides, siRNAs, splice-switching oligonucleotides, and nucleic acid aptamers, are being evaluated in clinical trials for the treatment of a variety of diseases. Synthesis of Therapeutic Oligonucleotides covers a broad range of topics in the field that are of high relevance to researchers, including the synthesis of natural and chemically modified oligonucleotides, the development of novel nucleic acid analogs, industrial scale synthesis and purification of oligonucleotides, and important aspects of chemistry, manufacturing, and controls (CMC). The aim is to provide new insights and inspire fresh ideas in nucleic acid chemistry that may ultimately lead to novel concepts and techniques and the discovery of more effective nucleic acid drugs. The book will be of high value for both established researchers in the field and students intending to specialize in nucleic acid chemistry research.
Oligonucleotide Synthesis
Oligonucleotide Synthesis - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Oligonucleotide Synthesis write by Piet Herdewijn. This book was released on 2008-02-04. Oligonucleotide Synthesis available in PDF, EPUB and Kindle. A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.
Therapeutic Oligonucleotides
Therapeutic Oligonucleotides - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Therapeutic Oligonucleotides write by Jens Kurreck. This book was released on 2008. Therapeutic Oligonucleotides available in PDF, EPUB and Kindle. This book provides a compelling overall update on current status of RNA interference
Protocols for Oligonucleotides and Analogs
Protocols for Oligonucleotides and Analogs - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Protocols for Oligonucleotides and Analogs write by Sudhir Agrawal. This book was released on 1993-08-31. Protocols for Oligonucleotides and Analogs available in PDF, EPUB and Kindle. When first conceived, not only was the aim of Protocols for Oligo nucleotides and Analogs to provide wide coverage of the ohgonuc- otide chemistry field for readers who are well versed within the field, but also to give investigators just entering into the field a new perspective. The very first book on this topic was edited and published by Michael Gait in 1984, in whose laboratory I encountered the newer aspects of oligonucleotide chemistry. Since then, oligonucleotide research has developed to such an extent that its uses extend far beyond basic studies, and now find wide application throughout clinical science as well. Until recently, the major application of oligonucleotides has been in the area of DNA-based diagnostic and "antisense oligonucleotid- based therapeutic approaches. However, oligonucleotides are now also being used as therapeutic agents and are thus frequently found in clinical trials in humans. Synthesis of unmodified oligonucleotides using automated synthe sizers has become a common practice in numerous laboratories. How ever, improvements on the existing techniques and the introduction of ever newer methods for oligonucleotide synthesis is constantly driving ahead in the leading research laboratories. And several new oligonucle otide analogs have been synthesized and studied for their individual prop erties in recent years. The present volume strives to bring the readers the most up-to-date information on the newest aspects of synthesis of oligo nucleotides and their analogs. A separate volume covers synthesis of oligonucleotide conjugates, along with most of the analytical techniques presently used for analysis of oligonucleotides.
Therapeutic Oligonucleotides
Therapeutic Oligonucleotides - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Therapeutic Oligonucleotides write by John Goodchild. This book was released on 2011-07-15. Therapeutic Oligonucleotides available in PDF, EPUB and Kindle. The field of oligonucleotide therapeutics research is ripe with the prospect of new discoveries. In Therapeutic Oligonucleotides: Methods and Protocols, a selection of established and emerging methods for the application of oligonucleotides as therapeutics are presented, all providing the tools needed to inspire great changes in the field. Divided into twenty-one chapters, this detailed volume meticulously describes vital protocols for optimizing and improving cell uptake, such as photochemical internalization, modified cell penetrating peptides, antibody conjugates, and nanoparticles. Other chapters address quantitation of RNA therapeutics in cells, assaying gene knockdown, selecting the best target site and synthesis of various modified oligonucleotides. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Therapeutic Oligonucleotides: Methods and Protocols serves as a timely resource for both professionals and novices pursuing research in this exciting and pioneering field.
