Use of Process Design and Metabolic Engineering to Enhance Bioconversion of Lignocellulosic Biomass and Glycerol to Biofuels - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Use of Process Design and Metabolic Engineering to Enhance Bioconversion of Lignocellulosic Biomass and Glycerol to Biofuels write by Chidozie Victor Agu. This book was released on 2016. Use of Process Design and Metabolic Engineering to Enhance Bioconversion of Lignocellulosic Biomass and Glycerol to Biofuels available in PDF, EPUB and Kindle. Recent efforts to reduce dependency on food-based substrates for industrial applications aim towards the use of inexpensive and readily available non-food based substrates such as lignocellulosic biomass (LB) and biodiesel-derived glycerol. Interestingly, the utilization of lignocellulosic sugars for biofuel production is contingent on the disruption of recalcitrant LB cell wall structure prior to enzyme hydrolysis. Disruption and hydrolysis processes generate lignocellulose-derived microbial inhibitory compounds (LDMIC) including acids, aldehydes and phenolics. Additionally, fermentation of glycerol to butanol, a next-generation biofuel, is hampered by the inability of Clostridium beijerinckii NCIMB 8052, a butanol fermentation workhorse, to efficiently metabolize glycerol. Therefore, this study investigated novel strategies for enhancing butanol and ethanol production through process design and metabolic engineering. Towards process design, the bacterium Cupriavidus basilensis ATCC®BAA-699 was used to detoxify 98% of the LDMIC present in acid-pretreated Miscanthus giganteus (MG) lignocellulosic biomass hydrolysates. Fermentation of the detoxified MG hydrolysates by C. beijerinckii resulted in 70%, 50%, and 73% improvement in acetone-butanol-ethanol (ABE) concentration, yield and productivity, respectively, when compared to the fermentation of undetoxified MG hydrolysates. The second objective was to explore metabolic engineering strategies to enhance glycerol utilization by C. beijerinckii and improve butanol production in the presence of LDMIC. To realize this objective, genes that encode glycerol dehydrogenases (Gldh) and dihydroxyacetone kinase (Dhak) in a hyper-glycerol utilizing bacterium (Clostridium pasteurianum ATCC 6013) were systematically cloned into C. beijerinckii. By over-expressing two C. pasteurianum Gldh genes (dhaD1+gldA1) as a fusion protein in C. beijerinckii, we achieved 50% increase in cell growth, ABE production (up to 40%), and enhanced rate of furfural detoxification (up to 68%) during the fermentation of furfural-challenged (4 to 6 g/L) glucose+glycerol medium. Further, co-expression of dhaD1+gldA1 resulted in significant payoff in cell growth (57%), glycerol consumption (14%), and ABE productivity (27.3%) compared to over-expression of a single Gldh. In parallel, while co-expression of dhak and gldA1 in C. beijerinckii improved glycerol consumption by 37% relative to the plasmid control, over-expression of all three genes (dhaD1+gldA1+dhak) improved butanol production by >50% in the presence of 5 and 6 g/L furfural relative to the plasmid control. Objective 3 aimed to develop a high-throughput alcohol dehydrogenase (ADH)-dependent assay for screening hyper- or hypo- butanol producing C. beijerinckii mutant libraries. Screening of the activities of ADHs from different microorganisms showed that Thermotoga hypogea derived ADH has ~7-fold activity towards butanol than ethanol. It was rationalized that T. hypogea ADH can be used to selectively quantify butanol in the presence ofethanol (e.g., in ABE broth). Objective 4 aimed to use allopurinol to inhibit xanthine dehydrogenase/oxidase and improve ethanol fermentation of LB hydrolysates by Saccharomyces cerevisiae. Allopurinol increased S. cerevisiae growth (19%), ethanol titer (21%), ethanol productivity (20%), ethanol yield (24%), and the chronological lifespan of S. cerevisiae (>16 h) during the fermentation of 100% corn stover hydrolysate. Taken together, this study encompasses novel strategies to enhance LB and glycerol utilization and potentially improve the economics of biobutanol and bioethanol production.
Metabolic Regulation and Metabolic Engineering for Biofuel and Biochemical Production
Metabolic Regulation and Metabolic Engineering for Biofuel and Biochemical Production - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Metabolic Regulation and Metabolic Engineering for Biofuel and Biochemical Production write by Kazuyuki Shimizu. This book was released on 2017-07-12. Metabolic Regulation and Metabolic Engineering for Biofuel and Biochemical Production available in PDF, EPUB and Kindle. The global warming problem is becoming critical year by year, causing climate disaster all over the world, where it has been believed that the CO2 gas emitted from the factories and the burning of fossil fuels may be one of the reasons of global warming. Moreover, the global stock of fossil fuels is limited, and may run out soon within several tens of years. Although wind, geo-thermal, and tide energies have been considered as clean energy sources, those depend on the land or sea locations and subject to the climate change. Biofuel and biochemical production from renewable bio-resources has thus been paid recent attention from environmental protection and energy production points of view, where the current chemical and energy producing plants can be also utilized with slight modification. The so-called 1st generation biofuels have been produced from corn starch and sugarcane in particular in USA and Brazil. However, this causes the problem of the so-called "food and energy issues" as the production scale increases. The 2nd generation biofuel production from lingo-cellulosic biomass or wastes has thus been paid recent attention. However, it requires energy intensive pretreatment for the degradation of lingo-cellulosic biomass, and the fermentation is slow due to low growth rate, and thus the productivity of biofuels and bio-chemicals is low. The 3rd generation biofuel production from photosynthetic organisms such as cyanobacteria and algae has been also paid attention, because such organisms can grow with only sun light and CO2 in the air, but the cell growth rate and thus the productivity of the fuels is significantly low. The main part or core of such production processes is the fermentation by micro-organisms. In particular, it is critical to properly understand the cell metabolism followed by the efficient metabolic engineering. The book gives comprehensive explanation of the cell metabolism and the metabolic regulation mechanisms of a variety of micro-organisms. Then the efficient metabolic engineering approaches are explained to properly design the microbial cell factories for the efficient cell growth and biofuel and biochemical production.
Lignocellulosic Biomass Refining for Second Generation Biofuel Production
Lignocellulosic Biomass Refining for Second Generation Biofuel Production - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Lignocellulosic Biomass Refining for Second Generation Biofuel Production write by Ponnusami V.. This book was released on 2023-07-14. Lignocellulosic Biomass Refining for Second Generation Biofuel Production available in PDF, EPUB and Kindle. Describes technological advancements for bioethanol production from lignocellulosic waste Provides a roadmap for the production and utilization of 2G biofuels Introduces the strategic role of metabolic engineering in the development of 2G biofuels Discusses technological advancements, life cycle assessment and prospects Explores novel potential lignocellulosic biomass for 2G biofuels
Genetic and Metabolic Engineering for Improved Biofuel Production from Lignocellulosic Biomass
Genetic and Metabolic Engineering for Improved Biofuel Production from Lignocellulosic Biomass - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Genetic and Metabolic Engineering for Improved Biofuel Production from Lignocellulosic Biomass write by Arindam Kuila. This book was released on 2020-02-19. Genetic and Metabolic Engineering for Improved Biofuel Production from Lignocellulosic Biomass available in PDF, EPUB and Kindle. Genetic and Metabolic Engineering for Improved Biofuel Production from Lignocellulosic Biomass describes the different aspects of biofuel production from lignocellulosic biomass. Each chapter presents different technological approaches for cost effective liquid biofuel production from agroresidues/biomass. Two chapters cover future direction and the possibilities of biomass-based biofuel production at the industrial level. The book provides a genetic and metabolic engineering approach for improved cellulase production and the potential of strains that can ferment both pentose and hexose sugars. The book also gives direction on how to overcome challenges for the further advancement of lignocellulosic biomass-based biofuel production. Covers genetic engineering approaches for higher cellulase production from fungi Includes genetic and metabolic engineering approaches for development of potential pentose and hexose fermenting strain which can tolerate high ethanol and toxic phenolic compounds Describe different bioreactors used in different steps of biomass-based biofuel production Outlines future prospects and potential of biofuel production from lignocellulosic biomass
Recent Advances in Bioconversion of Lignocellulose to Biofuels and Value Added Chemicals within the Biorefinery Concept
Recent Advances in Bioconversion of Lignocellulose to Biofuels and Value Added Chemicals within the Biorefinery Concept - read free eBook in online reader or directly download on the web page. Select files or add your book in reader. Download and read online ebook Recent Advances in Bioconversion of Lignocellulose to Biofuels and Value Added Chemicals within the Biorefinery Concept write by Edivaldo Ximenes Ferreira Filho. This book was released on 2020-05-19. Recent Advances in Bioconversion of Lignocellulose to Biofuels and Value Added Chemicals within the Biorefinery Concept available in PDF, EPUB and Kindle. Recent Advances in Bioconversion of Lignocellulose to Biofuels and Value Added Chemicals within the Biorefinery Concept covers the latest developments on biorefineries, along with their potential use for the transformation of residues into a broad range of more valuable products. Within this context, the book discusses the enzymatic conversion process of lignocellulosic biomass to generate fuels and other products in a unified approach. It focuses on new approaches to increase enzymatic production by microorganisms, the action of microbial inhibitors, and strategies for their removal. Furthermore, it outlines the benefits of this integrated approach for generating value-added products and the benefits to social and economic aspects, circular bio economy, HUBs and perspectives. Covers the mechanisms of enzymatic conversion of biomass into value-added products Discusses bioproducts derived from lignocellulose and their applications Includes discussions on design, development and the technologies needed for the sustainable manufacture of materials and chemicals Offers a techno-economic evaluation of biorefineries for integrated sustainability assessments Discusses the socioeconomic and cultural-economic perspectives of the lignocellulosic biorefinery Presents a virtual biorefinery as an integrated approach to evaluate the lignocellulose production chain
